Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris
Fusion proteins composed of a cellulose-binding domain from Neocallimastix patriciarum cellulase A and Candida antarctica lipase B were constructed using different linker peptides. The aim was to create proteolytically stable linkers that were able to join the functional modules without disrupting t...
Published in: | Protein Engineering, Design and Selection |
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2001
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fthighwire:oai:open-archive.highwire.org:proeng:14/9/711 2023-05-15T13:50:08+02:00 Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris Gustavsson, Malin Lehtiö, Janne Denman, Stuart Teeri, Tuula T. Hult, Karl Martinelle, Mats 2001-09-01 00:00:00.0 text/html http://peds.oxfordjournals.org/cgi/content/short/14/9/711 https://doi.org/10.1093/protein/14.9.711 en eng Oxford University Press http://peds.oxfordjournals.org/cgi/content/short/14/9/711 http://dx.doi.org/10.1093/protein/14.9.711 Copyright (C) 2001, Oxford University Press ORIGINAL ARTICLE TEXT 2001 fthighwire https://doi.org/10.1093/protein/14.9.711 2007-06-23T21:35:39Z Fusion proteins composed of a cellulose-binding domain from Neocallimastix patriciarum cellulase A and Candida antarctica lipase B were constructed using different linker peptides. The aim was to create proteolytically stable linkers that were able to join the functional modules without disrupting their function. Six fusion variants containing linkers of 4–44 residues were expressed in Pichia pastoris and analysed. Three variants were found to be stable throughout 7-day cultivations. The cellulose-binding capacities of fusion proteins containing short linkers were slightly lower compared with those containing long linkers. The lipase-specific activities of all variants, in solution or immobilized on to cellulose, were equal to that of the wild-type lipase. Text Antarc* Antarctica HighWire Press (Stanford University) Protein Engineering, Design and Selection 14 9 711 715 |
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Open Polar |
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HighWire Press (Stanford University) |
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fthighwire |
language |
English |
topic |
ORIGINAL ARTICLE |
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ORIGINAL ARTICLE Gustavsson, Malin Lehtiö, Janne Denman, Stuart Teeri, Tuula T. Hult, Karl Martinelle, Mats Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris |
topic_facet |
ORIGINAL ARTICLE |
description |
Fusion proteins composed of a cellulose-binding domain from Neocallimastix patriciarum cellulase A and Candida antarctica lipase B were constructed using different linker peptides. The aim was to create proteolytically stable linkers that were able to join the functional modules without disrupting their function. Six fusion variants containing linkers of 4–44 residues were expressed in Pichia pastoris and analysed. Three variants were found to be stable throughout 7-day cultivations. The cellulose-binding capacities of fusion proteins containing short linkers were slightly lower compared with those containing long linkers. The lipase-specific activities of all variants, in solution or immobilized on to cellulose, were equal to that of the wild-type lipase. |
format |
Text |
author |
Gustavsson, Malin Lehtiö, Janne Denman, Stuart Teeri, Tuula T. Hult, Karl Martinelle, Mats |
author_facet |
Gustavsson, Malin Lehtiö, Janne Denman, Stuart Teeri, Tuula T. Hult, Karl Martinelle, Mats |
author_sort |
Gustavsson, Malin |
title |
Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris |
title_short |
Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris |
title_full |
Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris |
title_fullStr |
Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris |
title_full_unstemmed |
Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris |
title_sort |
stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in pichia pastoris |
publisher |
Oxford University Press |
publishDate |
2001 |
url |
http://peds.oxfordjournals.org/cgi/content/short/14/9/711 https://doi.org/10.1093/protein/14.9.711 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://peds.oxfordjournals.org/cgi/content/short/14/9/711 http://dx.doi.org/10.1093/protein/14.9.711 |
op_rights |
Copyright (C) 2001, Oxford University Press |
op_doi |
https://doi.org/10.1093/protein/14.9.711 |
container_title |
Protein Engineering, Design and Selection |
container_volume |
14 |
container_issue |
9 |
container_start_page |
711 |
op_container_end_page |
715 |
_version_ |
1766253106712018944 |