Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton

• Published in: Journal of Plankton Research
• Main Authors: Bell, Michael V, Dick, James R, Anderson, Thomas R, Pond, David W
• Format: Text
• Language: English
• Published: Oxford University Press 2007
• Subjects: Article; Calanus finmarchicus; Euphausia superba
• Online Access: http://plankt.oxfordjournals.org/cgi/content/short/fbm025v1; https://doi.org/10.1093/plankt/fbm025

We investigated the ability of four species of marine zooplankton to synthesise polyunsaturated fatty acids (PUFAs) during pulse-chase tracer experiments. Liposomes containing a deuterium labelled precursor fatty acid, D 5 -18:3(n-3), were fed to female Calanus finmarchicus, Calanoides acutus, Dropanopus forcipatus and calyptopus larvae of Euphausia superba during ship-board experiments. Although all species of zooplankton readily ingested the liposomes and incorporated the D 5 -18:3(n-3) tracer into their somatic lipid pool, only negligible products of elongation and desaturation; D 5 -18:4(n-3), D 5 -20:5(n-3) and D 5 -22:6(n-3) were detected after 96 hour incubations. We conclude that the four species of marine zooplankton examined here are unable to synthesis PUFA at ecologically significant rates and certainly not in amounts sufficient to support growth and reproductive processes i.e. not in amounts sufficient to support growth and reproductive processes, under the conditions of this experiment