Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases (AARS) method
We obtained growth rates of the copepod Calanus finmarchicus at different locations across the North Atlantic between May 1998 and June 2004. Animals were incubated for 2 to 9 days, and fed either with natural food assemblages or cultured algae. During this period, we measured both somatic weight-sp...
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fthighwire:oai:open-archive.highwire.org:plankt:fbl049v1 2023-05-15T15:47:52+02:00 Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases (AARS) method Yebra, Lidia Hirst, Andrew G. Hernández-León, Santiago 2006-09-21 09:32:54.0 text/html http://plankt.oxfordjournals.org/cgi/content/short/fbl049v1 https://doi.org/10.1093/plankt/fbl049 en eng Oxford University Press http://plankt.oxfordjournals.org/cgi/content/short/fbl049v1 http://dx.doi.org/10.1093/plankt/fbl049 Copyright (C) 2006, Oxford University Press Article TEXT 2006 fthighwire https://doi.org/10.1093/plankt/fbl049 2016-11-16T18:35:37Z We obtained growth rates of the copepod Calanus finmarchicus at different locations across the North Atlantic between May 1998 and June 2004. Animals were incubated for 2 to 9 days, and fed either with natural food assemblages or cultured algae. During this period, we measured both somatic weight-specific growth rates (measured as protein change) and aminoacyl-tRNA synthetases (AARS) activity. We found a highly significant relationship between AARS activity and growth in protein content (R2= 0.55, p < 0.001). Significant AARS activity also occurred when growth was negative, the relationship predicting an AARS activity level <8.33 nmPPi·mg protein-1·h-1 when somatic growth is zero. This is because AARS activity is expected even when growth is negative, due to the continued protein turnover in the cells. The AARS method allowed for the first time the study of protein metabolism in overwintering Calanus finmarchicus . Our results showed that overwintering copepods had significantly lower values of AARS activity than non-diapausing animals (t = -3.51, p < 0.002). The AARS method opens the possibility to better understand physiology dynamics of deep water organisms (e.g. the beginning and end of diapause). Text Calanus finmarchicus North Atlantic Copepods HighWire Press (Stanford University) Journal of Plankton Research 28 12 1191 1198 |
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Article Yebra, Lidia Hirst, Andrew G. Hernández-León, Santiago Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases (AARS) method |
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Article |
description |
We obtained growth rates of the copepod Calanus finmarchicus at different locations across the North Atlantic between May 1998 and June 2004. Animals were incubated for 2 to 9 days, and fed either with natural food assemblages or cultured algae. During this period, we measured both somatic weight-specific growth rates (measured as protein change) and aminoacyl-tRNA synthetases (AARS) activity. We found a highly significant relationship between AARS activity and growth in protein content (R2= 0.55, p < 0.001). Significant AARS activity also occurred when growth was negative, the relationship predicting an AARS activity level <8.33 nmPPi·mg protein-1·h-1 when somatic growth is zero. This is because AARS activity is expected even when growth is negative, due to the continued protein turnover in the cells. The AARS method allowed for the first time the study of protein metabolism in overwintering Calanus finmarchicus . Our results showed that overwintering copepods had significantly lower values of AARS activity than non-diapausing animals (t = -3.51, p < 0.002). The AARS method opens the possibility to better understand physiology dynamics of deep water organisms (e.g. the beginning and end of diapause). |
format |
Text |
author |
Yebra, Lidia Hirst, Andrew G. Hernández-León, Santiago |
author_facet |
Yebra, Lidia Hirst, Andrew G. Hernández-León, Santiago |
author_sort |
Yebra, Lidia |
title |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases (AARS) method |
title_short |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases (AARS) method |
title_full |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases (AARS) method |
title_fullStr |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases (AARS) method |
title_full_unstemmed |
Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases (AARS) method |
title_sort |
assessment of calanus finmarchicus growth and dormancy using the aminoacyl-trna synthetases (aars) method |
publisher |
Oxford University Press |
publishDate |
2006 |
url |
http://plankt.oxfordjournals.org/cgi/content/short/fbl049v1 https://doi.org/10.1093/plankt/fbl049 |
genre |
Calanus finmarchicus North Atlantic Copepods |
genre_facet |
Calanus finmarchicus North Atlantic Copepods |
op_relation |
http://plankt.oxfordjournals.org/cgi/content/short/fbl049v1 http://dx.doi.org/10.1093/plankt/fbl049 |
op_rights |
Copyright (C) 2006, Oxford University Press |
op_doi |
https://doi.org/10.1093/plankt/fbl049 |
container_title |
Journal of Plankton Research |
container_volume |
28 |
container_issue |
12 |
container_start_page |
1191 |
op_container_end_page |
1198 |
_version_ |
1766382825490087936 |