Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton
We investigated the ability of four species of marine zooplankton to synthesize polyunsaturated fatty acids (PUFAs) during pulse-chase tracer experiments. Liposomes containing a deuterium labelled precursor fatty acid, D 5 -18:3n‐3, were fed to female Calanus finmarchicus, Calanoides acutus, Drepano...
Published in: | Journal of Plankton Research |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
Oxford University Press
2007
|
Subjects: | |
Online Access: | http://plankt.oxfordjournals.org/cgi/content/short/29/5/417 https://doi.org/10.1093/plankt/fbm025 |
id |
fthighwire:oai:open-archive.highwire.org:plankt:29/5/417 |
---|---|
record_format |
openpolar |
spelling |
fthighwire:oai:open-archive.highwire.org:plankt:29/5/417 2023-05-15T15:47:59+02:00 Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton Bell, Michael V. Dick, James R. Anderson, Thomas R. Pond, David W. 2007-05-01 00:00:00.0 text/html http://plankt.oxfordjournals.org/cgi/content/short/29/5/417 https://doi.org/10.1093/plankt/fbm025 en eng Oxford University Press http://plankt.oxfordjournals.org/cgi/content/short/29/5/417 http://dx.doi.org/10.1093/plankt/fbm025 Copyright (C) 2007, Oxford University Press ORIGINAL ARTICLES TEXT 2007 fthighwire https://doi.org/10.1093/plankt/fbm025 2013-05-26T12:44:19Z We investigated the ability of four species of marine zooplankton to synthesize polyunsaturated fatty acids (PUFAs) during pulse-chase tracer experiments. Liposomes containing a deuterium labelled precursor fatty acid, D 5 -18:3n‐3, were fed to female Calanus finmarchicus, Calanoides acutus, Drepanopus forcipatus and calyptopus larvae of Euphausia superba during ship-board experiments. Although all species of zooplankton readily ingested the liposomes and incorporated the D 5 -18:3n‐3 tracer into their somatic lipid pool, only negligible products of elongation and desaturation; D 5 -18:4n‐3, D 5 -20:5n‐3 and D 5 -22:6n‐3 were detected after 96 h incubations. We conclude that the four species of marine zooplankton examined here are unable to synthesize PUFA at ecologically significant rates and certainly not in amounts sufficient to support growth and reproductive processes. Text Calanus finmarchicus Euphausia superba HighWire Press (Stanford University) Journal of Plankton Research 29 5 417 422 |
institution |
Open Polar |
collection |
HighWire Press (Stanford University) |
op_collection_id |
fthighwire |
language |
English |
topic |
ORIGINAL ARTICLES |
spellingShingle |
ORIGINAL ARTICLES Bell, Michael V. Dick, James R. Anderson, Thomas R. Pond, David W. Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton |
topic_facet |
ORIGINAL ARTICLES |
description |
We investigated the ability of four species of marine zooplankton to synthesize polyunsaturated fatty acids (PUFAs) during pulse-chase tracer experiments. Liposomes containing a deuterium labelled precursor fatty acid, D 5 -18:3n‐3, were fed to female Calanus finmarchicus, Calanoides acutus, Drepanopus forcipatus and calyptopus larvae of Euphausia superba during ship-board experiments. Although all species of zooplankton readily ingested the liposomes and incorporated the D 5 -18:3n‐3 tracer into their somatic lipid pool, only negligible products of elongation and desaturation; D 5 -18:4n‐3, D 5 -20:5n‐3 and D 5 -22:6n‐3 were detected after 96 h incubations. We conclude that the four species of marine zooplankton examined here are unable to synthesize PUFA at ecologically significant rates and certainly not in amounts sufficient to support growth and reproductive processes. |
format |
Text |
author |
Bell, Michael V. Dick, James R. Anderson, Thomas R. Pond, David W. |
author_facet |
Bell, Michael V. Dick, James R. Anderson, Thomas R. Pond, David W. |
author_sort |
Bell, Michael V. |
title |
Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton |
title_short |
Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton |
title_full |
Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton |
title_fullStr |
Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton |
title_full_unstemmed |
Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton |
title_sort |
application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton |
publisher |
Oxford University Press |
publishDate |
2007 |
url |
http://plankt.oxfordjournals.org/cgi/content/short/29/5/417 https://doi.org/10.1093/plankt/fbm025 |
genre |
Calanus finmarchicus Euphausia superba |
genre_facet |
Calanus finmarchicus Euphausia superba |
op_relation |
http://plankt.oxfordjournals.org/cgi/content/short/29/5/417 http://dx.doi.org/10.1093/plankt/fbm025 |
op_rights |
Copyright (C) 2007, Oxford University Press |
op_doi |
https://doi.org/10.1093/plankt/fbm025 |
container_title |
Journal of Plankton Research |
container_volume |
29 |
container_issue |
5 |
container_start_page |
417 |
op_container_end_page |
422 |
_version_ |
1766382967573184512 |