Responses in pigmentation and anti-oxidant expression in Arctic Daphnia along gradients of DOC and UV exposure
Responses in carapace melanization and expression of the major anti-oxidant catalase (CAT) and glutathione transferase (GST) in Arctic Daphnia were assessed in enclosures along a gradient of dissolved organic carbon (DOC). This gradient was created by adding freeze-dried humic matter to 2 m3 UV-tran...
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2002
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fthighwire:oai:open-archive.highwire.org:plankt:24/10/1009 2023-05-15T14:58:38+02:00 Responses in pigmentation and anti-oxidant expression in Arctic Daphnia along gradients of DOC and UV exposure Hessen, Dag O. Borgeraas, Jan Ørbæk, Jon Børre 2002-10-01 00:00:00.0 text/html http://plankt.oxfordjournals.org/cgi/content/short/24/10/1009 https://doi.org/10.1093/plankt/24.10.1009 en eng Oxford University Press http://plankt.oxfordjournals.org/cgi/content/short/24/10/1009 http://dx.doi.org/10.1093/plankt/24.10.1009 Copyright (C) 2002, Oxford University Press ORIGINAL ARTICLES TEXT 2002 fthighwire https://doi.org/10.1093/plankt/24.10.1009 2013-05-28T00:00:24Z Responses in carapace melanization and expression of the major anti-oxidant catalase (CAT) and glutathione transferase (GST) in Arctic Daphnia were assessed in enclosures along a gradient of dissolved organic carbon (DOC). This gradient was created by adding freeze-dried humic matter to 2 m3 UV-transparent enclosures, yielding final nominal concentrations of 1, 2.5, 5 and 10 mg C l−1. The UV attenuation was strongly affected by additions of DOC, and attenuation coefficients at 320 nm increased from 3.0 in the control to approximately 3.5 and 11.0 m−1 in the 1 and 10 mg DOC treatments respectively. Most Daphnia showed pronounced carapace melanization, and the absorbance of short-wave radiation through the carapace was strongly related to the degree of melanization. Nevertheless, the different UV climate in the enclosures did not cause any short-term adaptation in Daphnia pigmentation over a 3 week period. The levels of CAT and GST were assessed over time in the control and at 10 mg DOC. These enzymes displayed opposite patterns, with somewhat lower activities of CAT at low DOC (control) relative to 10 mg DOC, while the opposite was found for GST. There was also a significant negative correlation between CAT and solar irradiation for GST in both bags, while no effects were found for GST. Text Arctic HighWire Press (Stanford University) Arctic Journal of Plankton Research 24 10 1009 1018 |
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HighWire Press (Stanford University) |
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English |
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ORIGINAL ARTICLES |
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ORIGINAL ARTICLES Hessen, Dag O. Borgeraas, Jan Ørbæk, Jon Børre Responses in pigmentation and anti-oxidant expression in Arctic Daphnia along gradients of DOC and UV exposure |
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ORIGINAL ARTICLES |
description |
Responses in carapace melanization and expression of the major anti-oxidant catalase (CAT) and glutathione transferase (GST) in Arctic Daphnia were assessed in enclosures along a gradient of dissolved organic carbon (DOC). This gradient was created by adding freeze-dried humic matter to 2 m3 UV-transparent enclosures, yielding final nominal concentrations of 1, 2.5, 5 and 10 mg C l−1. The UV attenuation was strongly affected by additions of DOC, and attenuation coefficients at 320 nm increased from 3.0 in the control to approximately 3.5 and 11.0 m−1 in the 1 and 10 mg DOC treatments respectively. Most Daphnia showed pronounced carapace melanization, and the absorbance of short-wave radiation through the carapace was strongly related to the degree of melanization. Nevertheless, the different UV climate in the enclosures did not cause any short-term adaptation in Daphnia pigmentation over a 3 week period. The levels of CAT and GST were assessed over time in the control and at 10 mg DOC. These enzymes displayed opposite patterns, with somewhat lower activities of CAT at low DOC (control) relative to 10 mg DOC, while the opposite was found for GST. There was also a significant negative correlation between CAT and solar irradiation for GST in both bags, while no effects were found for GST. |
format |
Text |
author |
Hessen, Dag O. Borgeraas, Jan Ørbæk, Jon Børre |
author_facet |
Hessen, Dag O. Borgeraas, Jan Ørbæk, Jon Børre |
author_sort |
Hessen, Dag O. |
title |
Responses in pigmentation and anti-oxidant expression in Arctic Daphnia along gradients of DOC and UV exposure |
title_short |
Responses in pigmentation and anti-oxidant expression in Arctic Daphnia along gradients of DOC and UV exposure |
title_full |
Responses in pigmentation and anti-oxidant expression in Arctic Daphnia along gradients of DOC and UV exposure |
title_fullStr |
Responses in pigmentation and anti-oxidant expression in Arctic Daphnia along gradients of DOC and UV exposure |
title_full_unstemmed |
Responses in pigmentation and anti-oxidant expression in Arctic Daphnia along gradients of DOC and UV exposure |
title_sort |
responses in pigmentation and anti-oxidant expression in arctic daphnia along gradients of doc and uv exposure |
publisher |
Oxford University Press |
publishDate |
2002 |
url |
http://plankt.oxfordjournals.org/cgi/content/short/24/10/1009 https://doi.org/10.1093/plankt/24.10.1009 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
http://plankt.oxfordjournals.org/cgi/content/short/24/10/1009 http://dx.doi.org/10.1093/plankt/24.10.1009 |
op_rights |
Copyright (C) 2002, Oxford University Press |
op_doi |
https://doi.org/10.1093/plankt/24.10.1009 |
container_title |
Journal of Plankton Research |
container_volume |
24 |
container_issue |
10 |
container_start_page |
1009 |
op_container_end_page |
1018 |
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1766330768514088960 |