Different Cytoplasmic Calcium Contents Among Three Species of Characeae
Internodal cells of three species of Characeae, Nitella flexilis , Nitella axilliformis and Chara corallina , were analyzed for the contents of Ca2+ and Mg2+ in the cytoplasm. To avoid contamination of Ca2+ from the cell wall and vacuole, the vacuolar sap was replaced with a sorbitol solution contai...
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fthighwire:oai:open-archive.highwire.org:pcellphys:42/6/620 2023-05-15T17:23:50+02:00 Different Cytoplasmic Calcium Contents Among Three Species of Characeae Tazawa, Masashi Kikuyama, Munehiro Okazaki, Yoshiji 2001-06-01 00:00:00.0 text/html http://pcp.oxfordjournals.org/cgi/content/short/42/6/620 https://doi.org/10.1093/pcp/pce077 en eng Oxford University Press http://pcp.oxfordjournals.org/cgi/content/short/42/6/620 http://dx.doi.org/10.1093/pcp/pce077 Copyright (C) 2001, The Japanese Society of Plant Physiologists Regular Papers TEXT 2001 fthighwire https://doi.org/10.1093/pcp/pce077 2007-06-24T08:08:26Z Internodal cells of three species of Characeae, Nitella flexilis , Nitella axilliformis and Chara corallina , were analyzed for the contents of Ca2+ and Mg2+ in the cytoplasm. To avoid contamination of Ca2+ from the cell wall and vacuole, the vacuolar sap was replaced with a sorbitol solution containing Sr2+ by the vacuolar perfusion method after the cell had been treated with Sr2+. No significant difference in the cytoplasmic content of Mg2+ was found among three species of Characeae, but significant differences in the cytoplasmic content of Ca2+ were observed among them. The cytoplasmic Ca2+ content of N. flexilis was 2.0 times that of N. axilliformis and 3.3 times that of C. corallina . The cytoplasmic drop was furthermore separated into two fractions: a chloroplast-free fraction and a chloroplast fraction. In the chloroplast-free fraction the Ca2+ content of N. flexilis was 2.3 times that of C. corallina and 2.0 times that of N. axilliformis , while the Mg2+ content was the same among the three species. In the chloroplast fraction N. flexilis contained about seven times more Ca2+ and about two times more Mg2+ than C. corallina . The difference in the cytoplasmic Ca2+ content was discussed in relation to the difference in the capacity for the hydration-induced Ca2+ release existing among the three species. Text Nitella flexilis HighWire Press (Stanford University) Plant and Cell Physiology 42 6 620 626 |
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Regular Papers |
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Regular Papers Tazawa, Masashi Kikuyama, Munehiro Okazaki, Yoshiji Different Cytoplasmic Calcium Contents Among Three Species of Characeae |
topic_facet |
Regular Papers |
description |
Internodal cells of three species of Characeae, Nitella flexilis , Nitella axilliformis and Chara corallina , were analyzed for the contents of Ca2+ and Mg2+ in the cytoplasm. To avoid contamination of Ca2+ from the cell wall and vacuole, the vacuolar sap was replaced with a sorbitol solution containing Sr2+ by the vacuolar perfusion method after the cell had been treated with Sr2+. No significant difference in the cytoplasmic content of Mg2+ was found among three species of Characeae, but significant differences in the cytoplasmic content of Ca2+ were observed among them. The cytoplasmic Ca2+ content of N. flexilis was 2.0 times that of N. axilliformis and 3.3 times that of C. corallina . The cytoplasmic drop was furthermore separated into two fractions: a chloroplast-free fraction and a chloroplast fraction. In the chloroplast-free fraction the Ca2+ content of N. flexilis was 2.3 times that of C. corallina and 2.0 times that of N. axilliformis , while the Mg2+ content was the same among the three species. In the chloroplast fraction N. flexilis contained about seven times more Ca2+ and about two times more Mg2+ than C. corallina . The difference in the cytoplasmic Ca2+ content was discussed in relation to the difference in the capacity for the hydration-induced Ca2+ release existing among the three species. |
format |
Text |
author |
Tazawa, Masashi Kikuyama, Munehiro Okazaki, Yoshiji |
author_facet |
Tazawa, Masashi Kikuyama, Munehiro Okazaki, Yoshiji |
author_sort |
Tazawa, Masashi |
title |
Different Cytoplasmic Calcium Contents Among Three Species of Characeae |
title_short |
Different Cytoplasmic Calcium Contents Among Three Species of Characeae |
title_full |
Different Cytoplasmic Calcium Contents Among Three Species of Characeae |
title_fullStr |
Different Cytoplasmic Calcium Contents Among Three Species of Characeae |
title_full_unstemmed |
Different Cytoplasmic Calcium Contents Among Three Species of Characeae |
title_sort |
different cytoplasmic calcium contents among three species of characeae |
publisher |
Oxford University Press |
publishDate |
2001 |
url |
http://pcp.oxfordjournals.org/cgi/content/short/42/6/620 https://doi.org/10.1093/pcp/pce077 |
genre |
Nitella flexilis |
genre_facet |
Nitella flexilis |
op_relation |
http://pcp.oxfordjournals.org/cgi/content/short/42/6/620 http://dx.doi.org/10.1093/pcp/pce077 |
op_rights |
Copyright (C) 2001, The Japanese Society of Plant Physiologists |
op_doi |
https://doi.org/10.1093/pcp/pce077 |
container_title |
Plant and Cell Physiology |
container_volume |
42 |
container_issue |
6 |
container_start_page |
620 |
op_container_end_page |
626 |
_version_ |
1766114284446679040 |