Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1
Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we h...
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fthighwire:oai:open-archive.highwire.org:nar:31/24/7247 2023-05-15T16:49:57+02:00 Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 Blondal, Thorarinn Hjorleifsdottir, Sigridur H. Fridjonsson, Olafur F. Ævarsson, Arnthor Skirnisdottir, Sigurlaug Hermannsdottir, Anna Gudny Hreggvidsson, Gudmundur O. Smith, Albert Vernon Kristjansson, Jakob K. 2003-12-15 00:00:00.0 text/html http://nar.oxfordjournals.org/cgi/content/short/31/24/7247 https://doi.org/10.1093/nar/gkg914 en eng Oxford University Press http://nar.oxfordjournals.org/cgi/content/short/31/24/7247 http://dx.doi.org/10.1093/nar/gkg914 Copyright (C) 2003, Oxford University Press Articles TEXT 2003 fthighwire https://doi.org/10.1093/nar/gkg914 2015-03-01T01:11:06Z Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of genes with potential use in biotechnology. We have also demonstrated the existence of thermostable counterparts of previously known bacteriophage enzymes. Here we describe a thermostable RNA ligase 1 from the thermophilic bacteriophage RM378 that infects the thermophilic eubacterium Rhodothermus marinus. The RM378 RNA ligase 1 has a temperature optimum of 60–64°C and it ligates both RNA and single‐stranded DNA. Its thermostability and ability to work under conditions of high temperature where nucleic acid secondary structures are removed makes it an ideal enzyme for RNA ligase‐mediated rapid amplification of cDNA ends (RLM‐RACE), and other RNA and DNA ligation applications. Text Iceland HighWire Press (Stanford University) Nucleic Acids Research 31 24 7247 7254 |
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Articles Blondal, Thorarinn Hjorleifsdottir, Sigridur H. Fridjonsson, Olafur F. Ævarsson, Arnthor Skirnisdottir, Sigurlaug Hermannsdottir, Anna Gudny Hreggvidsson, Gudmundur O. Smith, Albert Vernon Kristjansson, Jakob K. Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 |
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Articles |
description |
Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of genes with potential use in biotechnology. We have also demonstrated the existence of thermostable counterparts of previously known bacteriophage enzymes. Here we describe a thermostable RNA ligase 1 from the thermophilic bacteriophage RM378 that infects the thermophilic eubacterium Rhodothermus marinus. The RM378 RNA ligase 1 has a temperature optimum of 60–64°C and it ligates both RNA and single‐stranded DNA. Its thermostability and ability to work under conditions of high temperature where nucleic acid secondary structures are removed makes it an ideal enzyme for RNA ligase‐mediated rapid amplification of cDNA ends (RLM‐RACE), and other RNA and DNA ligation applications. |
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Text |
author |
Blondal, Thorarinn Hjorleifsdottir, Sigridur H. Fridjonsson, Olafur F. Ævarsson, Arnthor Skirnisdottir, Sigurlaug Hermannsdottir, Anna Gudny Hreggvidsson, Gudmundur O. Smith, Albert Vernon Kristjansson, Jakob K. |
author_facet |
Blondal, Thorarinn Hjorleifsdottir, Sigridur H. Fridjonsson, Olafur F. Ævarsson, Arnthor Skirnisdottir, Sigurlaug Hermannsdottir, Anna Gudny Hreggvidsson, Gudmundur O. Smith, Albert Vernon Kristjansson, Jakob K. |
author_sort |
Blondal, Thorarinn |
title |
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 |
title_short |
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 |
title_full |
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 |
title_fullStr |
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 |
title_full_unstemmed |
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 |
title_sort |
discovery and characterization of a thermostable bacteriophage rna ligase homologous to t4 rna ligase 1 |
publisher |
Oxford University Press |
publishDate |
2003 |
url |
http://nar.oxfordjournals.org/cgi/content/short/31/24/7247 https://doi.org/10.1093/nar/gkg914 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://nar.oxfordjournals.org/cgi/content/short/31/24/7247 http://dx.doi.org/10.1093/nar/gkg914 |
op_rights |
Copyright (C) 2003, Oxford University Press |
op_doi |
https://doi.org/10.1093/nar/gkg914 |
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Nucleic Acids Research |
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31 |
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24 |
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7247 |
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7254 |
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1766040124760522752 |