Comparative Genomics Reveals Adaptive Protein Evolution and a Possible Cytonuclear Incompatibility between European and American Eels
During the early stages of speciation, interspecific gene flow may be impeded by deleterious epistatic interactions in hybrids, which maintain parental allelic combinations at the speciation genes. The resulting semipermeable nature of the barrier to interspecific gene flow provides a valuable frame...
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fthighwire:oai:open-archive.highwire.org:molbiolevol:29/10/2909 2023-05-15T13:27:57+02:00 Comparative Genomics Reveals Adaptive Protein Evolution and a Possible Cytonuclear Incompatibility between European and American Eels Gagnaire, Pierre-Alexandre Normandeau, Eric Bernatchez, Louis 2012-10-01 00:00:00.0 text/html http://mbe.oxfordjournals.org/cgi/content/short/29/10/2909 https://doi.org/10.1093/molbev/mss076 en eng Oxford University Press http://mbe.oxfordjournals.org/cgi/content/short/29/10/2909 http://dx.doi.org/10.1093/molbev/mss076 Copyright (C) 2012, Society for Molecular Biology and Evolution Research Articles TEXT 2012 fthighwire https://doi.org/10.1093/molbev/mss076 2013-05-26T14:51:16Z During the early stages of speciation, interspecific gene flow may be impeded by deleterious epistatic interactions in hybrids, which maintain parental allelic combinations at the speciation genes. The resulting semipermeable nature of the barrier to interspecific gene flow provides a valuable framework to identify the genes involved in hybrid mortality or sterility, as well as the evolutionary mechanisms that initially caused their divergence. The two Atlantic eels Anguilla anguilla and A. rostrata are partially isolated sister species that naturally hybridize, but whose genetic basis of postzygotic isolation remains unknown. We collected high-throughput sequencing data from the transcriptomes of 58 individuals and discovered 94 genes showing differentially fixed mutations between species. Evidence for positive selection at nuclear diagnostic genes was obtained using multilocus extensions of the McDonald–Kreitman test with polymorphism data from each species. In contrast, mitochondrial protein-coding genes experienced strong purifying selection and mostly diverged at synonymous sites, except for the mt-atp6 gene, which showed an atypically high nonsynonymous to synonymous rate ratio. Nuclear-encoded protein interactors of the mt-atp6 gene in the ATP synthase complex were significantly overrepresented in the list of nuclear diagnostic genes. Further analysis of resequencing data showed that positive selection has operated at both the mt-atp6 gene and its nuclear interactor atp5c1 . These findings suggest that a cytonuclear incompatibility caused by a disruption of normal ATP synthase function in hybrids contributes to partial reproductive isolation between European and American eels. Text Anguilla anguilla HighWire Press (Stanford University) Molecular Biology and Evolution 29 10 2909 2919 |
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Research Articles Gagnaire, Pierre-Alexandre Normandeau, Eric Bernatchez, Louis Comparative Genomics Reveals Adaptive Protein Evolution and a Possible Cytonuclear Incompatibility between European and American Eels |
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Research Articles |
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During the early stages of speciation, interspecific gene flow may be impeded by deleterious epistatic interactions in hybrids, which maintain parental allelic combinations at the speciation genes. The resulting semipermeable nature of the barrier to interspecific gene flow provides a valuable framework to identify the genes involved in hybrid mortality or sterility, as well as the evolutionary mechanisms that initially caused their divergence. The two Atlantic eels Anguilla anguilla and A. rostrata are partially isolated sister species that naturally hybridize, but whose genetic basis of postzygotic isolation remains unknown. We collected high-throughput sequencing data from the transcriptomes of 58 individuals and discovered 94 genes showing differentially fixed mutations between species. Evidence for positive selection at nuclear diagnostic genes was obtained using multilocus extensions of the McDonald–Kreitman test with polymorphism data from each species. In contrast, mitochondrial protein-coding genes experienced strong purifying selection and mostly diverged at synonymous sites, except for the mt-atp6 gene, which showed an atypically high nonsynonymous to synonymous rate ratio. Nuclear-encoded protein interactors of the mt-atp6 gene in the ATP synthase complex were significantly overrepresented in the list of nuclear diagnostic genes. Further analysis of resequencing data showed that positive selection has operated at both the mt-atp6 gene and its nuclear interactor atp5c1 . These findings suggest that a cytonuclear incompatibility caused by a disruption of normal ATP synthase function in hybrids contributes to partial reproductive isolation between European and American eels. |
format |
Text |
author |
Gagnaire, Pierre-Alexandre Normandeau, Eric Bernatchez, Louis |
author_facet |
Gagnaire, Pierre-Alexandre Normandeau, Eric Bernatchez, Louis |
author_sort |
Gagnaire, Pierre-Alexandre |
title |
Comparative Genomics Reveals Adaptive Protein Evolution and a Possible Cytonuclear Incompatibility between European and American Eels |
title_short |
Comparative Genomics Reveals Adaptive Protein Evolution and a Possible Cytonuclear Incompatibility between European and American Eels |
title_full |
Comparative Genomics Reveals Adaptive Protein Evolution and a Possible Cytonuclear Incompatibility between European and American Eels |
title_fullStr |
Comparative Genomics Reveals Adaptive Protein Evolution and a Possible Cytonuclear Incompatibility between European and American Eels |
title_full_unstemmed |
Comparative Genomics Reveals Adaptive Protein Evolution and a Possible Cytonuclear Incompatibility between European and American Eels |
title_sort |
comparative genomics reveals adaptive protein evolution and a possible cytonuclear incompatibility between european and american eels |
publisher |
Oxford University Press |
publishDate |
2012 |
url |
http://mbe.oxfordjournals.org/cgi/content/short/29/10/2909 https://doi.org/10.1093/molbev/mss076 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
http://mbe.oxfordjournals.org/cgi/content/short/29/10/2909 http://dx.doi.org/10.1093/molbev/mss076 |
op_rights |
Copyright (C) 2012, Society for Molecular Biology and Evolution |
op_doi |
https://doi.org/10.1093/molbev/mss076 |
container_title |
Molecular Biology and Evolution |
container_volume |
29 |
container_issue |
10 |
container_start_page |
2909 |
op_container_end_page |
2919 |
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1766401339790721024 |