Genomic Organization and Evolution of the Vomeronasal Type 2 Receptor-Like (OlfC) Gene Clusters in Atlantic Salmon, Salmo salar

There are three major multigene superfamilies of olfactory receptors (OR, V1R, and V2R) in mammals. The ORs are expressed in the main olfactory organ, whereas the V1Rs and V2Rs are located in the vomeronasal organ. Fish only possess one olfactory organ in each nasal cavity, the olfactory rosette; th...

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Published in:Molecular Biology and Evolution
Main Authors: Johnstone, Kimberley A., Ciborowski, Kate L., Lubieniecki, Krzysztof P., Chow, William, Phillips, Ruth B., Koop, Ben F., Jordan, William C., Davidson, William S.
Format: Text
Language:English
Published: Oxford University Press 2009
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Online Access:http://mbe.oxfordjournals.org/cgi/content/short/26/5/1117
https://doi.org/10.1093/molbev/msp027
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spelling fthighwire:oai:open-archive.highwire.org:molbiolevol:26/5/1117 2023-05-15T15:30:53+02:00 Genomic Organization and Evolution of the Vomeronasal Type 2 Receptor-Like (OlfC) Gene Clusters in Atlantic Salmon, Salmo salar Johnstone, Kimberley A. Ciborowski, Kate L. Lubieniecki, Krzysztof P. Chow, William Phillips, Ruth B. Koop, Ben F. Jordan, William C. Davidson, William S. 2009-05-01 00:00:00.0 text/html http://mbe.oxfordjournals.org/cgi/content/short/26/5/1117 https://doi.org/10.1093/molbev/msp027 en eng Oxford University Press http://mbe.oxfordjournals.org/cgi/content/short/26/5/1117 http://dx.doi.org/10.1093/molbev/msp027 Copyright (C) 2009, Society for Molecular Biology and Evolution Research Articles TEXT 2009 fthighwire https://doi.org/10.1093/molbev/msp027 2009-07-03T20:45:59Z There are three major multigene superfamilies of olfactory receptors (OR, V1R, and V2R) in mammals. The ORs are expressed in the main olfactory organ, whereas the V1Rs and V2Rs are located in the vomeronasal organ. Fish only possess one olfactory organ in each nasal cavity, the olfactory rosette; therefore, it has been proposed that their V2R-like genes be classified as olfactory C family G protein-coupled receptors ( OlfC ). There are large variations in the sizes of OR gene repertoires. Previous studies have shown that fish have between 12 and 46 functional V2R-like genes, whereas humans have lost all functional V2Rs, and frog sp. have more than 240. Pseudogenization of V2R genes is a prevalent event across species. In the mouse and frog genomes, there are approximately double the number of pseudogenes compared with functional genes. An oligonucleotide probe was designed from a conserved sequence from four Atlantic salmon O lfC genes and used to screen the Atlantic salmon bacterial artificial chromosome (BAC) library. Hybridization-positive BACs were matched to fingerprint contigs, and representative BACs were shotgun cloned and sequenced. We identified 55 O lfC genes. Twenty-nine of the OlfC genes are classified as putatively functional genes and 26 as pseudogenes. The OlfC genes are found in two genomic clusters on chromosomes 9 and 20. Phylogenetic analysis revealed that the OlfC genes could be divided into 10 subfamilies, with nine of these subfamilies corresponding to subfamilies found in other teleosts and one being salmon specific. There is also a large expansion in the number of OlfC genes in one subfamily in Atlantic salmon. Subfamily gene expansions have been identified in other teleosts, and these differences in gene number reflect species-specific evolutionary requirements for olfaction. Total RNA was isolated from the olfactory epithelium and other tissues from a presmolt to examine the expression of the odorant genes. Several of the putative OlfC genes that we identified are expressed only in the ... Text Atlantic salmon Salmo salar HighWire Press (Stanford University) Molecular Biology and Evolution 26 5 1117 1125
institution Open Polar
collection HighWire Press (Stanford University)
op_collection_id fthighwire
language English
topic Research Articles
spellingShingle Research Articles
Johnstone, Kimberley A.
Ciborowski, Kate L.
Lubieniecki, Krzysztof P.
Chow, William
Phillips, Ruth B.
Koop, Ben F.
Jordan, William C.
Davidson, William S.
Genomic Organization and Evolution of the Vomeronasal Type 2 Receptor-Like (OlfC) Gene Clusters in Atlantic Salmon, Salmo salar
topic_facet Research Articles
description There are three major multigene superfamilies of olfactory receptors (OR, V1R, and V2R) in mammals. The ORs are expressed in the main olfactory organ, whereas the V1Rs and V2Rs are located in the vomeronasal organ. Fish only possess one olfactory organ in each nasal cavity, the olfactory rosette; therefore, it has been proposed that their V2R-like genes be classified as olfactory C family G protein-coupled receptors ( OlfC ). There are large variations in the sizes of OR gene repertoires. Previous studies have shown that fish have between 12 and 46 functional V2R-like genes, whereas humans have lost all functional V2Rs, and frog sp. have more than 240. Pseudogenization of V2R genes is a prevalent event across species. In the mouse and frog genomes, there are approximately double the number of pseudogenes compared with functional genes. An oligonucleotide probe was designed from a conserved sequence from four Atlantic salmon O lfC genes and used to screen the Atlantic salmon bacterial artificial chromosome (BAC) library. Hybridization-positive BACs were matched to fingerprint contigs, and representative BACs were shotgun cloned and sequenced. We identified 55 O lfC genes. Twenty-nine of the OlfC genes are classified as putatively functional genes and 26 as pseudogenes. The OlfC genes are found in two genomic clusters on chromosomes 9 and 20. Phylogenetic analysis revealed that the OlfC genes could be divided into 10 subfamilies, with nine of these subfamilies corresponding to subfamilies found in other teleosts and one being salmon specific. There is also a large expansion in the number of OlfC genes in one subfamily in Atlantic salmon. Subfamily gene expansions have been identified in other teleosts, and these differences in gene number reflect species-specific evolutionary requirements for olfaction. Total RNA was isolated from the olfactory epithelium and other tissues from a presmolt to examine the expression of the odorant genes. Several of the putative OlfC genes that we identified are expressed only in the ...
format Text
author Johnstone, Kimberley A.
Ciborowski, Kate L.
Lubieniecki, Krzysztof P.
Chow, William
Phillips, Ruth B.
Koop, Ben F.
Jordan, William C.
Davidson, William S.
author_facet Johnstone, Kimberley A.
Ciborowski, Kate L.
Lubieniecki, Krzysztof P.
Chow, William
Phillips, Ruth B.
Koop, Ben F.
Jordan, William C.
Davidson, William S.
author_sort Johnstone, Kimberley A.
title Genomic Organization and Evolution of the Vomeronasal Type 2 Receptor-Like (OlfC) Gene Clusters in Atlantic Salmon, Salmo salar
title_short Genomic Organization and Evolution of the Vomeronasal Type 2 Receptor-Like (OlfC) Gene Clusters in Atlantic Salmon, Salmo salar
title_full Genomic Organization and Evolution of the Vomeronasal Type 2 Receptor-Like (OlfC) Gene Clusters in Atlantic Salmon, Salmo salar
title_fullStr Genomic Organization and Evolution of the Vomeronasal Type 2 Receptor-Like (OlfC) Gene Clusters in Atlantic Salmon, Salmo salar
title_full_unstemmed Genomic Organization and Evolution of the Vomeronasal Type 2 Receptor-Like (OlfC) Gene Clusters in Atlantic Salmon, Salmo salar
title_sort genomic organization and evolution of the vomeronasal type 2 receptor-like (olfc) gene clusters in atlantic salmon, salmo salar
publisher Oxford University Press
publishDate 2009
url http://mbe.oxfordjournals.org/cgi/content/short/26/5/1117
https://doi.org/10.1093/molbev/msp027
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_relation http://mbe.oxfordjournals.org/cgi/content/short/26/5/1117
http://dx.doi.org/10.1093/molbev/msp027
op_rights Copyright (C) 2009, Society for Molecular Biology and Evolution
op_doi https://doi.org/10.1093/molbev/msp027
container_title Molecular Biology and Evolution
container_volume 26
container_issue 5
container_start_page 1117
op_container_end_page 1125
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