Experimental evaluation of genetic predator identification from saliva traces on wildlife kills
Identification of predators from saliva traces on game species and/or livestock kills is gaining increasing importance in wildlife management, particularly in areas where direct wildlife–human conflicts regularly occur. When the noninvasive sampling of hairs and scats is difficult, as with rare and...
Published in: | Journal of Mammalogy |
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Oxford University Press
2015
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fthighwire:oai:open-archive.highwire.org:jmammal:96/1/138 2023-05-15T15:50:25+02:00 Experimental evaluation of genetic predator identification from saliva traces on wildlife kills Harms, Verena Nowak, Carsten Carl, Susanne Muñoz-Fuentes, Violeta 2015-02-01 00:00:00.0 text/html http://jmammal.oxfordjournals.org/cgi/content/short/96/1/138 https://doi.org/10.1093/jmammal/gyu014 en eng Oxford University Press http://jmammal.oxfordjournals.org/cgi/content/short/96/1/138 http://dx.doi.org/10.1093/jmammal/gyu014 Copyright (C) 2015, Oxford University Press Feature Article TEXT 2015 fthighwire https://doi.org/10.1093/jmammal/gyu014 2016-11-16T18:39:41Z Identification of predators from saliva traces on game species and/or livestock kills is gaining increasing importance in wildlife management, particularly in areas where direct wildlife–human conflicts regularly occur. When the noninvasive sampling of hairs and scats is difficult, as with rare and elusive predators, saliva samples constitute a potentially useful source of DNA. To test the feasibility of this approach in obtaining an accurate genotype of the predator, we applied an experimental approach. Captive wolves ( Canis lupus ) and lynxes ( Lynx lynx ) were allowed to feed on freshly killed roe deer ( Capreolus capreolus ) pieces for 1min. After removal, pieces were sampled for saliva traces after 1, 24, and 48h. Microsatellite analysis revealed that error rates and amplification failure increased sharply over time. While samples collected after 1 and 24 h yielded > 83% complete genotypes, values dropped to < 50% for samples collected after 48h, of which 7% were incorrect even when consensus genotypes from 9 polymerase chain reactions were obtained. Our results stress the importance of rapid sampling after carcass detection, as well as implementing a multiple-tubes approach when using microsatellite markers for genetic predator identification based on saliva traces. Text Canis lupus Lynx HighWire Press (Stanford University) Journal of Mammalogy 96 1 138 143 |
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HighWire Press (Stanford University) |
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fthighwire |
language |
English |
topic |
Feature Article |
spellingShingle |
Feature Article Harms, Verena Nowak, Carsten Carl, Susanne Muñoz-Fuentes, Violeta Experimental evaluation of genetic predator identification from saliva traces on wildlife kills |
topic_facet |
Feature Article |
description |
Identification of predators from saliva traces on game species and/or livestock kills is gaining increasing importance in wildlife management, particularly in areas where direct wildlife–human conflicts regularly occur. When the noninvasive sampling of hairs and scats is difficult, as with rare and elusive predators, saliva samples constitute a potentially useful source of DNA. To test the feasibility of this approach in obtaining an accurate genotype of the predator, we applied an experimental approach. Captive wolves ( Canis lupus ) and lynxes ( Lynx lynx ) were allowed to feed on freshly killed roe deer ( Capreolus capreolus ) pieces for 1min. After removal, pieces were sampled for saliva traces after 1, 24, and 48h. Microsatellite analysis revealed that error rates and amplification failure increased sharply over time. While samples collected after 1 and 24 h yielded > 83% complete genotypes, values dropped to < 50% for samples collected after 48h, of which 7% were incorrect even when consensus genotypes from 9 polymerase chain reactions were obtained. Our results stress the importance of rapid sampling after carcass detection, as well as implementing a multiple-tubes approach when using microsatellite markers for genetic predator identification based on saliva traces. |
format |
Text |
author |
Harms, Verena Nowak, Carsten Carl, Susanne Muñoz-Fuentes, Violeta |
author_facet |
Harms, Verena Nowak, Carsten Carl, Susanne Muñoz-Fuentes, Violeta |
author_sort |
Harms, Verena |
title |
Experimental evaluation of genetic predator identification from saliva traces on wildlife kills |
title_short |
Experimental evaluation of genetic predator identification from saliva traces on wildlife kills |
title_full |
Experimental evaluation of genetic predator identification from saliva traces on wildlife kills |
title_fullStr |
Experimental evaluation of genetic predator identification from saliva traces on wildlife kills |
title_full_unstemmed |
Experimental evaluation of genetic predator identification from saliva traces on wildlife kills |
title_sort |
experimental evaluation of genetic predator identification from saliva traces on wildlife kills |
publisher |
Oxford University Press |
publishDate |
2015 |
url |
http://jmammal.oxfordjournals.org/cgi/content/short/96/1/138 https://doi.org/10.1093/jmammal/gyu014 |
genre |
Canis lupus Lynx |
genre_facet |
Canis lupus Lynx |
op_relation |
http://jmammal.oxfordjournals.org/cgi/content/short/96/1/138 http://dx.doi.org/10.1093/jmammal/gyu014 |
op_rights |
Copyright (C) 2015, Oxford University Press |
op_doi |
https://doi.org/10.1093/jmammal/gyu014 |
container_title |
Journal of Mammalogy |
container_volume |
96 |
container_issue |
1 |
container_start_page |
138 |
op_container_end_page |
143 |
_version_ |
1766385365196734464 |