Evaluation of 15 Candidate Genes for Dilated Cardiomyopathy in the Newfoundland Dog
Dilated cardiomyopathy (DCM) is a disease of the myocardium, which causes heart failure and premature death. It has been described in humans and several domestic animals. In the Newfoundland dog, DCM is an autosomal dominant disease with late onset and reduced penetrance. We analyzed 15 candidate ge...
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Oxford University Press
2007
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fthighwire:oai:open-archive.highwire.org:jhered:esm090v1 2023-05-15T17:17:47+02:00 Evaluation of 15 Candidate Genes for Dilated Cardiomyopathy in the Newfoundland Dog Wiersma, Anje C. Stabej, Polona Leegwater, Peter A. J. Van Oost, Bernard A. Ollier, William E. Dukes-McEwan, Joanna 2007-11-12 05:56:57.0 text/html http://jhered.oxfordjournals.org/cgi/content/short/esm090v1 https://doi.org/10.1093/jhered/esm090 en eng Oxford University Press http://jhered.oxfordjournals.org/cgi/content/short/esm090v1 http://dx.doi.org/10.1093/jhered/esm090 Copyright (C) 2007, American Genetic Association Article TEXT 2007 fthighwire https://doi.org/10.1093/jhered/esm090 2013-05-28T10:23:43Z Dilated cardiomyopathy (DCM) is a disease of the myocardium, which causes heart failure and premature death. It has been described in humans and several domestic animals. In the Newfoundland dog, DCM is an autosomal dominant disease with late onset and reduced penetrance. We analyzed 15 candidate genes for their involvement in DCM in the Newfoundland dog. Polymorphic microsatellite markers and single Nucleotide Polymorphisms were genotyped in 4 families of Newfoundland dogs segregating dilated cardiomyopathy for the genes encoding α-cardiac actin ( ACTC ), caveolin ( CAVI ), cysteine-rich protein 3 ( CSRP3 ), LIM-domain binding factor 3 ( LDB3 ), desmin ( DES ), lamin A/C ( LMNA ), myosin heavy polypeptide 7 ( MYH7 ), delta-sarcoglycan ( SGCD ), troponin I ( TNNTI3 ), troponin T ( TNNT2 ), alpha-tropomyosin ( TPMI ), titin ( TTN ) and vinculin ( VCL ). A Logarithm of the odds (LOD) score of less than −2.0 in 2-point linkage analysis indicated exclusion of all but 2 genes, encoding CSRP3 and DES . A (LOD) score between −1.5 and −2.0 for CSRP3 and DES makes these genes unlikely causes of DCM in this dog breed. For the phospholamban ( PLN ) and titin cap ( TTN ) genes, a direct mutation screening approach was used. DNA sequence analysis of all exons showed no evidence that these genes are involved in DCM in the Newfoundland dog. Text Newfoundland HighWire Press (Stanford University) Journal of Heredity 99 1 73 80 |
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Article Wiersma, Anje C. Stabej, Polona Leegwater, Peter A. J. Van Oost, Bernard A. Ollier, William E. Dukes-McEwan, Joanna Evaluation of 15 Candidate Genes for Dilated Cardiomyopathy in the Newfoundland Dog |
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Dilated cardiomyopathy (DCM) is a disease of the myocardium, which causes heart failure and premature death. It has been described in humans and several domestic animals. In the Newfoundland dog, DCM is an autosomal dominant disease with late onset and reduced penetrance. We analyzed 15 candidate genes for their involvement in DCM in the Newfoundland dog. Polymorphic microsatellite markers and single Nucleotide Polymorphisms were genotyped in 4 families of Newfoundland dogs segregating dilated cardiomyopathy for the genes encoding α-cardiac actin ( ACTC ), caveolin ( CAVI ), cysteine-rich protein 3 ( CSRP3 ), LIM-domain binding factor 3 ( LDB3 ), desmin ( DES ), lamin A/C ( LMNA ), myosin heavy polypeptide 7 ( MYH7 ), delta-sarcoglycan ( SGCD ), troponin I ( TNNTI3 ), troponin T ( TNNT2 ), alpha-tropomyosin ( TPMI ), titin ( TTN ) and vinculin ( VCL ). A Logarithm of the odds (LOD) score of less than −2.0 in 2-point linkage analysis indicated exclusion of all but 2 genes, encoding CSRP3 and DES . A (LOD) score between −1.5 and −2.0 for CSRP3 and DES makes these genes unlikely causes of DCM in this dog breed. For the phospholamban ( PLN ) and titin cap ( TTN ) genes, a direct mutation screening approach was used. DNA sequence analysis of all exons showed no evidence that these genes are involved in DCM in the Newfoundland dog. |
format |
Text |
author |
Wiersma, Anje C. Stabej, Polona Leegwater, Peter A. J. Van Oost, Bernard A. Ollier, William E. Dukes-McEwan, Joanna |
author_facet |
Wiersma, Anje C. Stabej, Polona Leegwater, Peter A. J. Van Oost, Bernard A. Ollier, William E. Dukes-McEwan, Joanna |
author_sort |
Wiersma, Anje C. |
title |
Evaluation of 15 Candidate Genes for Dilated Cardiomyopathy in the Newfoundland Dog |
title_short |
Evaluation of 15 Candidate Genes for Dilated Cardiomyopathy in the Newfoundland Dog |
title_full |
Evaluation of 15 Candidate Genes for Dilated Cardiomyopathy in the Newfoundland Dog |
title_fullStr |
Evaluation of 15 Candidate Genes for Dilated Cardiomyopathy in the Newfoundland Dog |
title_full_unstemmed |
Evaluation of 15 Candidate Genes for Dilated Cardiomyopathy in the Newfoundland Dog |
title_sort |
evaluation of 15 candidate genes for dilated cardiomyopathy in the newfoundland dog |
publisher |
Oxford University Press |
publishDate |
2007 |
url |
http://jhered.oxfordjournals.org/cgi/content/short/esm090v1 https://doi.org/10.1093/jhered/esm090 |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_relation |
http://jhered.oxfordjournals.org/cgi/content/short/esm090v1 http://dx.doi.org/10.1093/jhered/esm090 |
op_rights |
Copyright (C) 2007, American Genetic Association |
op_doi |
https://doi.org/10.1093/jhered/esm090 |
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Journal of Heredity |
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99 |
container_issue |
1 |
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73 |
op_container_end_page |
80 |
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1766085355543461888 |