Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon
We describe the inheritance of 460 PCR-based loci in the polyploid-derived pink salmon ( Oncorhynchus gorbuscha ) genome using gynogenetic haploid embryos. We detected a length polymorphism in a growth hormone gene ( GH-2 ) intron that is caused by an 81 bp insertion homologous to the 3' end of...
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fthighwire:oai:open-archive.highwire.org:jhered:90/2/289 2023-05-15T17:52:51+02:00 Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon Spruell, P Pilgrim, KL Greene, BA Habicht, C Knudsen, KL Lindner, KR Olsen, JB Sage, GK Seeb, JE Allendorf, FW 1999-03-01 00:00:00.0 text/html http://jhered.oxfordjournals.org/cgi/content/short/90/2/289 https://doi.org/10.1093/jhered/90.2.289 en eng Oxford University Press http://jhered.oxfordjournals.org/cgi/content/short/90/2/289 http://dx.doi.org/10.1093/jhered/90.2.289 Copyright (C) 1999, American Genetic Association Articles TEXT 1999 fthighwire https://doi.org/10.1093/jhered/90.2.289 2007-06-24T15:31:29Z We describe the inheritance of 460 PCR-based loci in the polyploid-derived pink salmon ( Oncorhynchus gorbuscha ) genome using gynogenetic haploid embryos. We detected a length polymorphism in a growth hormone gene ( GH-2 ) intron that is caused by an 81 bp insertion homologous to the 3' end of the salmonid short interspersed repetitive element (SINE) Smal I. Such insertion polymorphisms within species bring into question the use of SINEs as phylogenetic markers. We confirmed that a microsatellite locus encodes a PCR-null allele that is responsible for an apparent deficit of heterozygotes in a population sample from Prince William Sound. Another set of microsatellite primers amplified alleles of the same molecular weight from both loci of a duplicated pair. In our analysis of several PCR-based multilocus techniques, we failed to detect evidence of comigrating fragments produced by duplicated loci. Segregation analysis of PCR-based markers using gynogenetic haploid embryos ensures that the interpretation of molecular variation is not complicated by heterozygosity, diploidy, or gene duplication. We urge investigators to test the inheritance of polymorphism in salmonids prior to using them to measure genetic variation. Text Oncorhynchus gorbuscha Pink salmon HighWire Press (Stanford University) Journal of Heredity 90 2 289 296 |
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HighWire Press (Stanford University) |
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English |
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Articles |
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Articles Spruell, P Pilgrim, KL Greene, BA Habicht, C Knudsen, KL Lindner, KR Olsen, JB Sage, GK Seeb, JE Allendorf, FW Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon |
topic_facet |
Articles |
description |
We describe the inheritance of 460 PCR-based loci in the polyploid-derived pink salmon ( Oncorhynchus gorbuscha ) genome using gynogenetic haploid embryos. We detected a length polymorphism in a growth hormone gene ( GH-2 ) intron that is caused by an 81 bp insertion homologous to the 3' end of the salmonid short interspersed repetitive element (SINE) Smal I. Such insertion polymorphisms within species bring into question the use of SINEs as phylogenetic markers. We confirmed that a microsatellite locus encodes a PCR-null allele that is responsible for an apparent deficit of heterozygotes in a population sample from Prince William Sound. Another set of microsatellite primers amplified alleles of the same molecular weight from both loci of a duplicated pair. In our analysis of several PCR-based multilocus techniques, we failed to detect evidence of comigrating fragments produced by duplicated loci. Segregation analysis of PCR-based markers using gynogenetic haploid embryos ensures that the interpretation of molecular variation is not complicated by heterozygosity, diploidy, or gene duplication. We urge investigators to test the inheritance of polymorphism in salmonids prior to using them to measure genetic variation. |
format |
Text |
author |
Spruell, P Pilgrim, KL Greene, BA Habicht, C Knudsen, KL Lindner, KR Olsen, JB Sage, GK Seeb, JE Allendorf, FW |
author_facet |
Spruell, P Pilgrim, KL Greene, BA Habicht, C Knudsen, KL Lindner, KR Olsen, JB Sage, GK Seeb, JE Allendorf, FW |
author_sort |
Spruell, P |
title |
Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon |
title_short |
Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon |
title_full |
Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon |
title_fullStr |
Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon |
title_full_unstemmed |
Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon |
title_sort |
inheritance of nuclear dna markers in gynogenetic haploid pink salmon |
publisher |
Oxford University Press |
publishDate |
1999 |
url |
http://jhered.oxfordjournals.org/cgi/content/short/90/2/289 https://doi.org/10.1093/jhered/90.2.289 |
genre |
Oncorhynchus gorbuscha Pink salmon |
genre_facet |
Oncorhynchus gorbuscha Pink salmon |
op_relation |
http://jhered.oxfordjournals.org/cgi/content/short/90/2/289 http://dx.doi.org/10.1093/jhered/90.2.289 |
op_rights |
Copyright (C) 1999, American Genetic Association |
op_doi |
https://doi.org/10.1093/jhered/90.2.289 |
container_title |
Journal of Heredity |
container_volume |
90 |
container_issue |
2 |
container_start_page |
289 |
op_container_end_page |
296 |
_version_ |
1766160590554791936 |