Physiological and molecular diversity of feather moss associative N2-fixing cyanobacteria
Cyanobacteria colonizing the feather moss Pleurozium schreberi were isolated from moss samples collected in northern Sweden and subjected to physiological and molecular characterization. Morphological studies of isolated and moss-associated cyanobacteria were carried out by light microscopy. Molecul...
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2005
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fthighwire:oai:open-archive.highwire.org:jexbot:eri309v1 2023-05-15T17:44:24+02:00 Physiological and molecular diversity of feather moss associative N2-fixing cyanobacteria Gentili, Francesco Nilsson, Marie-Charlotte Zackrisson, Olle DeLuca, Thomas H. Sellstedt, Anita 2005-11-01 08:14:19.0 text/html http://jxb.oxfordjournals.org/cgi/content/short/eri309v1 https://doi.org/10.1093/jxb/eri309 en eng Oxford University Press http://jxb.oxfordjournals.org/cgi/content/short/eri309v1 http://dx.doi.org/10.1093/jxb/eri309 Copyright (C) 2005, Society for Experimental Biology RESEARCH PAPER TEXT 2005 fthighwire https://doi.org/10.1093/jxb/eri309 2013-05-27T01:13:39Z Cyanobacteria colonizing the feather moss Pleurozium schreberi were isolated from moss samples collected in northern Sweden and subjected to physiological and molecular characterization. Morphological studies of isolated and moss-associated cyanobacteria were carried out by light microscopy. Molecular tools were used for cyanobacteria identification, and a reconstitution experiment of the association between non-associative mosses and cyanobacteria was conducted. The influence of temperature on N 2 fixation in the different cyanobacterial isolates and the influence of light and temperature on N 2 -fixation rates in the moss were studied using the acetylene reduction assay. Two different cyanobacteria were effectively isolated from P. schreberi : Nostoc sp. and Calothrix sp. A third genus, Stigonema sp. was identified by microscopy, but could not be isolated. The Nostoc sp. was found to fix N 2 at lower temperatures than Calothrix sp. Nostoc sp. and Stigonema sp. were the predominant cyanobacteria colonizing the moss. The attempt to reconstitute the association between the moss and cyanobacteria was successful. The two isolated genera of cyanobacteria in feather moss samples collected in northern Sweden differ in their temperature optima, which may have important ecological implications. Text Northern Sweden HighWire Press (Stanford University) Journal of Experimental Botany 56 422 3121 3127 |
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HighWire Press (Stanford University) |
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English |
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RESEARCH PAPER |
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RESEARCH PAPER Gentili, Francesco Nilsson, Marie-Charlotte Zackrisson, Olle DeLuca, Thomas H. Sellstedt, Anita Physiological and molecular diversity of feather moss associative N2-fixing cyanobacteria |
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RESEARCH PAPER |
description |
Cyanobacteria colonizing the feather moss Pleurozium schreberi were isolated from moss samples collected in northern Sweden and subjected to physiological and molecular characterization. Morphological studies of isolated and moss-associated cyanobacteria were carried out by light microscopy. Molecular tools were used for cyanobacteria identification, and a reconstitution experiment of the association between non-associative mosses and cyanobacteria was conducted. The influence of temperature on N 2 fixation in the different cyanobacterial isolates and the influence of light and temperature on N 2 -fixation rates in the moss were studied using the acetylene reduction assay. Two different cyanobacteria were effectively isolated from P. schreberi : Nostoc sp. and Calothrix sp. A third genus, Stigonema sp. was identified by microscopy, but could not be isolated. The Nostoc sp. was found to fix N 2 at lower temperatures than Calothrix sp. Nostoc sp. and Stigonema sp. were the predominant cyanobacteria colonizing the moss. The attempt to reconstitute the association between the moss and cyanobacteria was successful. The two isolated genera of cyanobacteria in feather moss samples collected in northern Sweden differ in their temperature optima, which may have important ecological implications. |
format |
Text |
author |
Gentili, Francesco Nilsson, Marie-Charlotte Zackrisson, Olle DeLuca, Thomas H. Sellstedt, Anita |
author_facet |
Gentili, Francesco Nilsson, Marie-Charlotte Zackrisson, Olle DeLuca, Thomas H. Sellstedt, Anita |
author_sort |
Gentili, Francesco |
title |
Physiological and molecular diversity of feather moss associative N2-fixing cyanobacteria |
title_short |
Physiological and molecular diversity of feather moss associative N2-fixing cyanobacteria |
title_full |
Physiological and molecular diversity of feather moss associative N2-fixing cyanobacteria |
title_fullStr |
Physiological and molecular diversity of feather moss associative N2-fixing cyanobacteria |
title_full_unstemmed |
Physiological and molecular diversity of feather moss associative N2-fixing cyanobacteria |
title_sort |
physiological and molecular diversity of feather moss associative n2-fixing cyanobacteria |
publisher |
Oxford University Press |
publishDate |
2005 |
url |
http://jxb.oxfordjournals.org/cgi/content/short/eri309v1 https://doi.org/10.1093/jxb/eri309 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
http://jxb.oxfordjournals.org/cgi/content/short/eri309v1 http://dx.doi.org/10.1093/jxb/eri309 |
op_rights |
Copyright (C) 2005, Society for Experimental Biology |
op_doi |
https://doi.org/10.1093/jxb/eri309 |
container_title |
Journal of Experimental Botany |
container_volume |
56 |
container_issue |
422 |
container_start_page |
3121 |
op_container_end_page |
3127 |
_version_ |
1766146613298855936 |