Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR
The energy status and the phosphate metabolism of Prasiola crisp during and after desiccation stress was investigated by in vivo 31P NMR. The effect of desiccation was simulated by addition of the nonionic osmoticum PEG 200 (polyethylene glycol). Photosynthesis and respiration were effectively inhib...
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Oxford University Press
1996
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fthighwire:oai:open-archive.highwire.org:jexbot:47/2/241 2023-05-15T14:00:53+02:00 Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR Bock, C. Jacob, A. Kirst, G. O. Leibfritz, D. Mayer, A. 1996-02-01 00:00:00.0 text/html http://jxb.oxfordjournals.org/cgi/content/short/47/2/241 https://doi.org/10.1093/jxb/47.2.241 en eng Oxford University Press http://jxb.oxfordjournals.org/cgi/content/short/47/2/241 http://dx.doi.org/10.1093/jxb/47.2.241 Copyright (C) 1996, Society for Experimental Biology Research Papers TEXT 1996 fthighwire https://doi.org/10.1093/jxb/47.2.241 2007-06-23T20:43:04Z The energy status and the phosphate metabolism of Prasiola crisp during and after desiccation stress was investigated by in vivo 31P NMR. The effect of desiccation was simulated by addition of the nonionic osmoticum PEG 200 (polyethylene glycol). Photosynthesis and respiration were effectively inhibited under these conditions. The most notable changes in the in vivo 31P NMR spectra were an increase in the cytoplasmic inorganic phosphate signal after PEG stress, a decrease in the polyphosphates and a lowfield shift of the core polyphosphate signal followed by an appearance of extracellular inorganic phosphate. Cytoplasmic pH remained almost constant during stress. After a return to control conditions, photosynthesis and respiration recovered within 4 h as well as the concentrations of the phosphorus metabolites. An as yet unassigned phosphate signal increased in the phosphodiester region of the NMR spectra. Simultaneousty, the polyphosphate signal recovered in intensity and chemical shift. It is suggested that phosphate metabolism and complexation of cations to polyphosphates may play an important role in the distinct desiccation tolerance of P. crispa . Text Antarc* Antarctic HighWire Press (Stanford University) Antarctic The Antarctic Journal of Experimental Botany 47 2 241 249 |
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HighWire Press (Stanford University) |
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English |
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Research Papers |
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Research Papers Bock, C. Jacob, A. Kirst, G. O. Leibfritz, D. Mayer, A. Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR |
topic_facet |
Research Papers |
description |
The energy status and the phosphate metabolism of Prasiola crisp during and after desiccation stress was investigated by in vivo 31P NMR. The effect of desiccation was simulated by addition of the nonionic osmoticum PEG 200 (polyethylene glycol). Photosynthesis and respiration were effectively inhibited under these conditions. The most notable changes in the in vivo 31P NMR spectra were an increase in the cytoplasmic inorganic phosphate signal after PEG stress, a decrease in the polyphosphates and a lowfield shift of the core polyphosphate signal followed by an appearance of extracellular inorganic phosphate. Cytoplasmic pH remained almost constant during stress. After a return to control conditions, photosynthesis and respiration recovered within 4 h as well as the concentrations of the phosphorus metabolites. An as yet unassigned phosphate signal increased in the phosphodiester region of the NMR spectra. Simultaneousty, the polyphosphate signal recovered in intensity and chemical shift. It is suggested that phosphate metabolism and complexation of cations to polyphosphates may play an important role in the distinct desiccation tolerance of P. crispa . |
format |
Text |
author |
Bock, C. Jacob, A. Kirst, G. O. Leibfritz, D. Mayer, A. |
author_facet |
Bock, C. Jacob, A. Kirst, G. O. Leibfritz, D. Mayer, A. |
author_sort |
Bock, C. |
title |
Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR |
title_short |
Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR |
title_full |
Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR |
title_fullStr |
Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR |
title_full_unstemmed |
Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR |
title_sort |
metabolic changes of the antarctic green alga prasiola crispa subjected to water stress investigated by in vivo 31p nmr |
publisher |
Oxford University Press |
publishDate |
1996 |
url |
http://jxb.oxfordjournals.org/cgi/content/short/47/2/241 https://doi.org/10.1093/jxb/47.2.241 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://jxb.oxfordjournals.org/cgi/content/short/47/2/241 http://dx.doi.org/10.1093/jxb/47.2.241 |
op_rights |
Copyright (C) 1996, Society for Experimental Biology |
op_doi |
https://doi.org/10.1093/jxb/47.2.241 |
container_title |
Journal of Experimental Botany |
container_volume |
47 |
container_issue |
2 |
container_start_page |
241 |
op_container_end_page |
249 |
_version_ |
1766270270299963392 |