Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR

The energy status and the phosphate metabolism of Prasiola crisp during and after desiccation stress was investigated by in vivo 31P NMR. The effect of desiccation was simulated by addition of the nonionic osmoticum PEG 200 (polyethylene glycol). Photosynthesis and respiration were effectively inhib...

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Published in:Journal of Experimental Botany
Main Authors: Bock, C., Jacob, A., Kirst, G. O., Leibfritz, D., Mayer, A.
Format: Text
Language:English
Published: Oxford University Press 1996
Subjects:
Online Access:http://jxb.oxfordjournals.org/cgi/content/short/47/2/241
https://doi.org/10.1093/jxb/47.2.241
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spelling fthighwire:oai:open-archive.highwire.org:jexbot:47/2/241 2023-05-15T14:00:53+02:00 Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR Bock, C. Jacob, A. Kirst, G. O. Leibfritz, D. Mayer, A. 1996-02-01 00:00:00.0 text/html http://jxb.oxfordjournals.org/cgi/content/short/47/2/241 https://doi.org/10.1093/jxb/47.2.241 en eng Oxford University Press http://jxb.oxfordjournals.org/cgi/content/short/47/2/241 http://dx.doi.org/10.1093/jxb/47.2.241 Copyright (C) 1996, Society for Experimental Biology Research Papers TEXT 1996 fthighwire https://doi.org/10.1093/jxb/47.2.241 2007-06-23T20:43:04Z The energy status and the phosphate metabolism of Prasiola crisp during and after desiccation stress was investigated by in vivo 31P NMR. The effect of desiccation was simulated by addition of the nonionic osmoticum PEG 200 (polyethylene glycol). Photosynthesis and respiration were effectively inhibited under these conditions. The most notable changes in the in vivo 31P NMR spectra were an increase in the cytoplasmic inorganic phosphate signal after PEG stress, a decrease in the polyphosphates and a lowfield shift of the core polyphosphate signal followed by an appearance of extracellular inorganic phosphate. Cytoplasmic pH remained almost constant during stress. After a return to control conditions, photosynthesis and respiration recovered within 4 h as well as the concentrations of the phosphorus metabolites. An as yet unassigned phosphate signal increased in the phosphodiester region of the NMR spectra. Simultaneousty, the polyphosphate signal recovered in intensity and chemical shift. It is suggested that phosphate metabolism and complexation of cations to polyphosphates may play an important role in the distinct desiccation tolerance of P. crispa . Text Antarc* Antarctic HighWire Press (Stanford University) Antarctic The Antarctic Journal of Experimental Botany 47 2 241 249
institution Open Polar
collection HighWire Press (Stanford University)
op_collection_id fthighwire
language English
topic Research Papers
spellingShingle Research Papers
Bock, C.
Jacob, A.
Kirst, G. O.
Leibfritz, D.
Mayer, A.
Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR
topic_facet Research Papers
description The energy status and the phosphate metabolism of Prasiola crisp during and after desiccation stress was investigated by in vivo 31P NMR. The effect of desiccation was simulated by addition of the nonionic osmoticum PEG 200 (polyethylene glycol). Photosynthesis and respiration were effectively inhibited under these conditions. The most notable changes in the in vivo 31P NMR spectra were an increase in the cytoplasmic inorganic phosphate signal after PEG stress, a decrease in the polyphosphates and a lowfield shift of the core polyphosphate signal followed by an appearance of extracellular inorganic phosphate. Cytoplasmic pH remained almost constant during stress. After a return to control conditions, photosynthesis and respiration recovered within 4 h as well as the concentrations of the phosphorus metabolites. An as yet unassigned phosphate signal increased in the phosphodiester region of the NMR spectra. Simultaneousty, the polyphosphate signal recovered in intensity and chemical shift. It is suggested that phosphate metabolism and complexation of cations to polyphosphates may play an important role in the distinct desiccation tolerance of P. crispa .
format Text
author Bock, C.
Jacob, A.
Kirst, G. O.
Leibfritz, D.
Mayer, A.
author_facet Bock, C.
Jacob, A.
Kirst, G. O.
Leibfritz, D.
Mayer, A.
author_sort Bock, C.
title Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR
title_short Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR
title_full Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR
title_fullStr Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR
title_full_unstemmed Metabolic changes of the Antarctic green alga Prasiola crispa subjected to water stress investigated by in vivo 31P NMR
title_sort metabolic changes of the antarctic green alga prasiola crispa subjected to water stress investigated by in vivo 31p nmr
publisher Oxford University Press
publishDate 1996
url http://jxb.oxfordjournals.org/cgi/content/short/47/2/241
https://doi.org/10.1093/jxb/47.2.241
geographic Antarctic
The Antarctic
geographic_facet Antarctic
The Antarctic
genre Antarc*
Antarctic
genre_facet Antarc*
Antarctic
op_relation http://jxb.oxfordjournals.org/cgi/content/short/47/2/241
http://dx.doi.org/10.1093/jxb/47.2.241
op_rights Copyright (C) 1996, Society for Experimental Biology
op_doi https://doi.org/10.1093/jxb/47.2.241
container_title Journal of Experimental Botany
container_volume 47
container_issue 2
container_start_page 241
op_container_end_page 249
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