Exposure to critical thermal maxima causes oxidative stress in hearts of white- but not red-blooded Antarctic notothenioid fishes
Antarctic icefishes have a significantly lower critical thermal maximum (CTmax) compared to most red-blooded notothenioid fishes. We hypothesized that the lower thermal tolerance of icefishes compared to red-blooded notothenioids may stem from a greater vulnerability to oxidative stress as temperatu...
Published in: | Journal of Experimental Biology |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Company of Biologists
2012
|
Subjects: | |
Online Access: | http://jeb.biologists.org/cgi/content/short/jeb.071811v1 https://doi.org/10.1242/jeb.071811 |
id |
fthighwire:oai:open-archive.highwire.org:jexbio:jeb.071811v1 |
---|---|
record_format |
openpolar |
spelling |
fthighwire:oai:open-archive.highwire.org:jexbio:jeb.071811v1 2023-05-15T13:52:24+02:00 Exposure to critical thermal maxima causes oxidative stress in hearts of white- but not red-blooded Antarctic notothenioid fishes Mueller, Irina A. Devor, Devin P. Grim, Jeffrey M. Beers, Jody M. Crockett, Elizabeth L. O'Brien, Kristin M. 2012-07-18 07:55:08.0 text/html http://jeb.biologists.org/cgi/content/short/jeb.071811v1 https://doi.org/10.1242/jeb.071811 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/jeb.071811v1 http://dx.doi.org/10.1242/jeb.071811 Copyright (C) 2012, Company of Biologists Research Article TEXT 2012 fthighwire https://doi.org/10.1242/jeb.071811 2015-02-28T14:04:22Z Antarctic icefishes have a significantly lower critical thermal maximum (CTmax) compared to most red-blooded notothenioid fishes. We hypothesized that the lower thermal tolerance of icefishes compared to red-blooded notothenioids may stem from a greater vulnerability to oxidative stress as temperature increases. Oxidative muscles of icefishes have high volume densities of mitochondria, rich in polyunsaturated fatty acids, which can promote the production of reactive oxygen species (ROS). Moreover, icefishes have lower levels of antioxidants compared to red-blooded species. To test our hypothesis, we measured levels of oxidized proteins and lipids, and transcript levels and maximal activities of antioxidants in heart ventricle and oxidative pectoral adductor muscle of icefishes and red-blooded notothenioids held at 0°C and exposed to their CTmax. Levels of oxidized proteins and lipids increased in heart ventricle of some icefishes but not in red-blooded species in response to warming, and not in pectoral adductor muscle of any species. Thus, increases in oxidative damage in heart ventricles may contribute to the reduced thermal tolerance of icefishes. Despite an increase in oxidative damage in hearts of icefishes, neither transcript levels nor activities of antioxidants increased, nor did they increase in any tissue of any species in response to exposure to CTmax. Rather, transcript levels of the enzyme superoxide dismutase (SOD) decreased in hearts of icefishes and the activity of SOD decreased in hearts of the red-blooded species Gobionotothen gibberifrons . These data suggest that notothenioids may have lost the ability to elevate levels of antioxidants in response to heat stress. Text Antarc* Antarctic HighWire Press (Stanford University) Antarctic Journal of Experimental Biology |
institution |
Open Polar |
collection |
HighWire Press (Stanford University) |
op_collection_id |
fthighwire |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Mueller, Irina A. Devor, Devin P. Grim, Jeffrey M. Beers, Jody M. Crockett, Elizabeth L. O'Brien, Kristin M. Exposure to critical thermal maxima causes oxidative stress in hearts of white- but not red-blooded Antarctic notothenioid fishes |
topic_facet |
Research Article |
description |
Antarctic icefishes have a significantly lower critical thermal maximum (CTmax) compared to most red-blooded notothenioid fishes. We hypothesized that the lower thermal tolerance of icefishes compared to red-blooded notothenioids may stem from a greater vulnerability to oxidative stress as temperature increases. Oxidative muscles of icefishes have high volume densities of mitochondria, rich in polyunsaturated fatty acids, which can promote the production of reactive oxygen species (ROS). Moreover, icefishes have lower levels of antioxidants compared to red-blooded species. To test our hypothesis, we measured levels of oxidized proteins and lipids, and transcript levels and maximal activities of antioxidants in heart ventricle and oxidative pectoral adductor muscle of icefishes and red-blooded notothenioids held at 0°C and exposed to their CTmax. Levels of oxidized proteins and lipids increased in heart ventricle of some icefishes but not in red-blooded species in response to warming, and not in pectoral adductor muscle of any species. Thus, increases in oxidative damage in heart ventricles may contribute to the reduced thermal tolerance of icefishes. Despite an increase in oxidative damage in hearts of icefishes, neither transcript levels nor activities of antioxidants increased, nor did they increase in any tissue of any species in response to exposure to CTmax. Rather, transcript levels of the enzyme superoxide dismutase (SOD) decreased in hearts of icefishes and the activity of SOD decreased in hearts of the red-blooded species Gobionotothen gibberifrons . These data suggest that notothenioids may have lost the ability to elevate levels of antioxidants in response to heat stress. |
format |
Text |
author |
Mueller, Irina A. Devor, Devin P. Grim, Jeffrey M. Beers, Jody M. Crockett, Elizabeth L. O'Brien, Kristin M. |
author_facet |
Mueller, Irina A. Devor, Devin P. Grim, Jeffrey M. Beers, Jody M. Crockett, Elizabeth L. O'Brien, Kristin M. |
author_sort |
Mueller, Irina A. |
title |
Exposure to critical thermal maxima causes oxidative stress in hearts of white- but not red-blooded Antarctic notothenioid fishes |
title_short |
Exposure to critical thermal maxima causes oxidative stress in hearts of white- but not red-blooded Antarctic notothenioid fishes |
title_full |
Exposure to critical thermal maxima causes oxidative stress in hearts of white- but not red-blooded Antarctic notothenioid fishes |
title_fullStr |
Exposure to critical thermal maxima causes oxidative stress in hearts of white- but not red-blooded Antarctic notothenioid fishes |
title_full_unstemmed |
Exposure to critical thermal maxima causes oxidative stress in hearts of white- but not red-blooded Antarctic notothenioid fishes |
title_sort |
exposure to critical thermal maxima causes oxidative stress in hearts of white- but not red-blooded antarctic notothenioid fishes |
publisher |
Company of Biologists |
publishDate |
2012 |
url |
http://jeb.biologists.org/cgi/content/short/jeb.071811v1 https://doi.org/10.1242/jeb.071811 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://jeb.biologists.org/cgi/content/short/jeb.071811v1 http://dx.doi.org/10.1242/jeb.071811 |
op_rights |
Copyright (C) 2012, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.071811 |
container_title |
Journal of Experimental Biology |
_version_ |
1766256687606398976 |