Responses to Hypersaline Exposure in the Euryhaline Crayfish Pacifastacus Leniusculus: I. The Interaction between Ionic and Acid-base Regulation
Haemolymph iono- and osmoregulation and acid-base balance were recorded after 48 h exposure at 15 °C to a range of increasing ambient salinities (0, 25, 50 and 75% sea water) in the euryhaline crayfish Pacifastacus leniusculus (Dana). Except for K+, concentrations of all measured inorganic ions and...
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fthighwire:oai:open-archive.highwire.org:jexbio:99/1/425 2023-05-15T15:52:46+02:00 Responses to Hypersaline Exposure in the Euryhaline Crayfish Pacifastacus Leniusculus: I. The Interaction between Ionic and Acid-base Regulation WHEATLY, MICHÈLE G. MCMAHON, B. R. 1982-08-01 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/99/1/425 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/99/1/425 Copyright (C) 1982, Company of Biologists Journal Articles TEXT 1982 fthighwire 2015-02-28T14:10:25Z Haemolymph iono- and osmoregulation and acid-base balance were recorded after 48 h exposure at 15 °C to a range of increasing ambient salinities (0, 25, 50 and 75% sea water) in the euryhaline crayfish Pacifastacus leniusculus (Dana). Except for K+, concentrations of all measured inorganic ions and osmolality were significantly elevated in 50 and 75% SW. When compared with ambient changes there was evidence of a transition from hyperto hypoionic regulation above 44% SW. Ca2+ was regulated for a constant blood-medium difference. A progressive reduction in total CO 2 was recorded; pH was maintained except in 75% SW where a haemolymph acidosis developed. To permit calculation of CO 2 tension (P CO CO2 ), carbon dioxide solubility coefficient ( CO 2 ) and the apparent first dissociation constant of carbonic acid (p K' 1 ) were experimentally determined in vitro . CO 2 decreased progressively with acclimation salinity but was unaffected by circulating protein. pK' 1 decreased as a function both of physiological pH and increasing haemolymph ionic strength. P CO CO2 calculated using these empirical constants, progressively decreased with high-salinity acclimation. The resulting hypocapnic alkalosis was partially offset by a metabolic acidosis, whose correlation with extracellular anisosmotic and intracellular isosmotic regulation is discussed. Text Carbonic acid HighWire Press (Stanford University) |
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Journal Articles |
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Journal Articles WHEATLY, MICHÈLE G. MCMAHON, B. R. Responses to Hypersaline Exposure in the Euryhaline Crayfish Pacifastacus Leniusculus: I. The Interaction between Ionic and Acid-base Regulation |
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Journal Articles |
description |
Haemolymph iono- and osmoregulation and acid-base balance were recorded after 48 h exposure at 15 °C to a range of increasing ambient salinities (0, 25, 50 and 75% sea water) in the euryhaline crayfish Pacifastacus leniusculus (Dana). Except for K+, concentrations of all measured inorganic ions and osmolality were significantly elevated in 50 and 75% SW. When compared with ambient changes there was evidence of a transition from hyperto hypoionic regulation above 44% SW. Ca2+ was regulated for a constant blood-medium difference. A progressive reduction in total CO 2 was recorded; pH was maintained except in 75% SW where a haemolymph acidosis developed. To permit calculation of CO 2 tension (P CO CO2 ), carbon dioxide solubility coefficient ( CO 2 ) and the apparent first dissociation constant of carbonic acid (p K' 1 ) were experimentally determined in vitro . CO 2 decreased progressively with acclimation salinity but was unaffected by circulating protein. pK' 1 decreased as a function both of physiological pH and increasing haemolymph ionic strength. P CO CO2 calculated using these empirical constants, progressively decreased with high-salinity acclimation. The resulting hypocapnic alkalosis was partially offset by a metabolic acidosis, whose correlation with extracellular anisosmotic and intracellular isosmotic regulation is discussed. |
format |
Text |
author |
WHEATLY, MICHÈLE G. MCMAHON, B. R. |
author_facet |
WHEATLY, MICHÈLE G. MCMAHON, B. R. |
author_sort |
WHEATLY, MICHÈLE G. |
title |
Responses to Hypersaline Exposure in the Euryhaline Crayfish Pacifastacus Leniusculus: I. The Interaction between Ionic and Acid-base Regulation |
title_short |
Responses to Hypersaline Exposure in the Euryhaline Crayfish Pacifastacus Leniusculus: I. The Interaction between Ionic and Acid-base Regulation |
title_full |
Responses to Hypersaline Exposure in the Euryhaline Crayfish Pacifastacus Leniusculus: I. The Interaction between Ionic and Acid-base Regulation |
title_fullStr |
Responses to Hypersaline Exposure in the Euryhaline Crayfish Pacifastacus Leniusculus: I. The Interaction between Ionic and Acid-base Regulation |
title_full_unstemmed |
Responses to Hypersaline Exposure in the Euryhaline Crayfish Pacifastacus Leniusculus: I. The Interaction between Ionic and Acid-base Regulation |
title_sort |
responses to hypersaline exposure in the euryhaline crayfish pacifastacus leniusculus: i. the interaction between ionic and acid-base regulation |
publisher |
Company of Biologists |
publishDate |
1982 |
url |
http://jeb.biologists.org/cgi/content/short/99/1/425 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
http://jeb.biologists.org/cgi/content/short/99/1/425 |
op_rights |
Copyright (C) 1982, Company of Biologists |
_version_ |
1766387864030937088 |