Red blood cell carbonic anhydrase mediates oxygen delivery via the Root effect in red drum
Oxygen (O 2 ) and carbon dioxide (CO 2 ) transport are tightly coupled in many fishes as a result of the presence of Root effect hemoglobins (Hb), whereby reduced pH reduces O 2 binding even at high O 2 tensions. Red blood cell carbonic anhydrase (RBC CA) activity limits the rate of intracellular ac...
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fthighwire:oai:open-archive.highwire.org:jexbio:223/22/jeb232991 2023-05-15T18:06:02+02:00 Red blood cell carbonic anhydrase mediates oxygen delivery via the Root effect in red drum Dichiera, Angelina M. Esbaugh, Andrew J. 2020-11-26 00:22:35.0 text/html http://jeb.biologists.org/cgi/content/short/223/22/jeb232991 https://doi.org/10.1242/jeb.232991 en eng The Company of Biologists Ltd http://jeb.biologists.org/cgi/content/short/223/22/jeb232991 http://dx.doi.org/10.1242/jeb.232991 Copyright (C) 2020, Company of Biologists RESEARCH ARTICLE TEXT 2020 fthighwire https://doi.org/10.1242/jeb.232991 2020-12-27T18:18:34Z Oxygen (O 2 ) and carbon dioxide (CO 2 ) transport are tightly coupled in many fishes as a result of the presence of Root effect hemoglobins (Hb), whereby reduced pH reduces O 2 binding even at high O 2 tensions. Red blood cell carbonic anhydrase (RBC CA) activity limits the rate of intracellular acidification, yet its role in O 2 delivery has been downplayed. We developed an in vitro assay to manipulate RBC CA activity while measuring Hb-O 2 offloading following a physiologically relevant CO 2 -induced acidification. RBC CA activity in red drum ( Sciaenops ocellatus ) was inhibited with ethoxzolamide by 53.7±0.5%, which prompted a significant reduction in O 2 offloading rate by 54.3±5.4% ( P= 0.0206, two-tailed paired t -test; n =7). Conversely, a 2.03-fold increase in RBC CA activity prompted a 2.14-fold increase in O 2 offloading rate ( P <0.001, two-tailed paired t -test; n= 8). This approximately 1:1 relationship between RBC CA activity and Hb-O 2 offloading rate coincided with a similar allometric scaling exponent for RBC CA activity and maximum metabolic rate. Together, our data suggest that RBC CA is rate limiting for O 2 delivery in red drum. Text Red drum Sciaenops ocellatus HighWire Press (Stanford University) Journal of Experimental Biology 223 22 |
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HighWire Press (Stanford University) |
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English |
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RESEARCH ARTICLE |
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RESEARCH ARTICLE Dichiera, Angelina M. Esbaugh, Andrew J. Red blood cell carbonic anhydrase mediates oxygen delivery via the Root effect in red drum |
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RESEARCH ARTICLE |
description |
Oxygen (O 2 ) and carbon dioxide (CO 2 ) transport are tightly coupled in many fishes as a result of the presence of Root effect hemoglobins (Hb), whereby reduced pH reduces O 2 binding even at high O 2 tensions. Red blood cell carbonic anhydrase (RBC CA) activity limits the rate of intracellular acidification, yet its role in O 2 delivery has been downplayed. We developed an in vitro assay to manipulate RBC CA activity while measuring Hb-O 2 offloading following a physiologically relevant CO 2 -induced acidification. RBC CA activity in red drum ( Sciaenops ocellatus ) was inhibited with ethoxzolamide by 53.7±0.5%, which prompted a significant reduction in O 2 offloading rate by 54.3±5.4% ( P= 0.0206, two-tailed paired t -test; n =7). Conversely, a 2.03-fold increase in RBC CA activity prompted a 2.14-fold increase in O 2 offloading rate ( P <0.001, two-tailed paired t -test; n= 8). This approximately 1:1 relationship between RBC CA activity and Hb-O 2 offloading rate coincided with a similar allometric scaling exponent for RBC CA activity and maximum metabolic rate. Together, our data suggest that RBC CA is rate limiting for O 2 delivery in red drum. |
format |
Text |
author |
Dichiera, Angelina M. Esbaugh, Andrew J. |
author_facet |
Dichiera, Angelina M. Esbaugh, Andrew J. |
author_sort |
Dichiera, Angelina M. |
title |
Red blood cell carbonic anhydrase mediates oxygen delivery via the Root effect in red drum |
title_short |
Red blood cell carbonic anhydrase mediates oxygen delivery via the Root effect in red drum |
title_full |
Red blood cell carbonic anhydrase mediates oxygen delivery via the Root effect in red drum |
title_fullStr |
Red blood cell carbonic anhydrase mediates oxygen delivery via the Root effect in red drum |
title_full_unstemmed |
Red blood cell carbonic anhydrase mediates oxygen delivery via the Root effect in red drum |
title_sort |
red blood cell carbonic anhydrase mediates oxygen delivery via the root effect in red drum |
publisher |
The Company of Biologists Ltd |
publishDate |
2020 |
url |
http://jeb.biologists.org/cgi/content/short/223/22/jeb232991 https://doi.org/10.1242/jeb.232991 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
http://jeb.biologists.org/cgi/content/short/223/22/jeb232991 http://dx.doi.org/10.1242/jeb.232991 |
op_rights |
Copyright (C) 2020, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.232991 |
container_title |
Journal of Experimental Biology |
container_volume |
223 |
container_issue |
22 |
_version_ |
1766177594523254784 |