Activation of oxygen-responsive pathways is associated with altered protein metabolism in Arctic char exposed to hypoxia
Fish exposed to fluctuating oxygen concentrations often alter their metabolism and/or behaviour to survive. Hypoxia tolerance is typically associated with the ability to reduce energy demand by supressing metabolic processes such as protein synthesis. Arctic char is amongst the most sensitive salmon...
Published in: | Journal of Experimental Biology |
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fthighwire:oai:open-archive.highwire.org:jexbio:222/22/jeb203901 2023-05-15T14:53:12+02:00 Activation of oxygen-responsive pathways is associated with altered protein metabolism in Arctic char exposed to hypoxia Cassidy, Alicia A. Lamarre, Simon G. 2019-11-21 01:16:35.0 text/html http://jeb.biologists.org/cgi/content/short/222/22/jeb203901 https://doi.org/10.1242/jeb.203901 en eng The Company of Biologists Ltd http://jeb.biologists.org/cgi/content/short/222/22/jeb203901 http://dx.doi.org/10.1242/jeb.203901 Copyright (C) 2019, Company of Biologists RESEARCH ARTICLE TEXT 2019 fthighwire https://doi.org/10.1242/jeb.203901 2019-12-09T12:59:53Z Fish exposed to fluctuating oxygen concentrations often alter their metabolism and/or behaviour to survive. Hypoxia tolerance is typically associated with the ability to reduce energy demand by supressing metabolic processes such as protein synthesis. Arctic char is amongst the most sensitive salmonid to hypoxia, and typically engage in avoidance behaviour when faced with lack of oxygen. We hypothesized that a sensitive species will still have the ability (albeit reduced) to regulate molecular mechanisms during hypoxia. We investigated the tissue-specific response of protein metabolism during hypoxia. Little is known about protein degradation pathways during hypoxia in fish and we predict that protein degradation pathways are differentially regulated and play a role in the hypoxia response. We also studied the regulation of oxygen-responsive cellular signalling pathways [hypoxia inducible factor (HIF), unfolded protein response (UPR) and mTOR pathways] since most of what we know comes from studies on cancerous mammalian cell lines. Arctic char were exposed to cumulative graded hypoxia trials for 3 h at four air saturation levels (100%, 50%, 30% and 15%). The rate of protein synthesis was measured using a flooding dose technique, whereas protein degradation and signalling pathways were assessed by measuring transcripts and phosphorylation of target proteins. Protein synthesis decreased in all tissues measured (liver, muscle, gill, digestive system) except for the heart. Salmonid hearts have preferential access to oxygen through a well-developed coronary artery, therefore the heart is likely to be the last tissue to become hypoxic. Autophagy markers were upregulated in the liver, whereas protein degradation markers were downregulated in the heart during hypoxia. Further work is needed to determine the effects of a decrease in protein degradation on a hypoxic salmonid heart. Our study showed that protein metabolism in Arctic char is altered in a tissue-specific fashion during graded hypoxia, which is in ... Text Arctic HighWire Press (Stanford University) Arctic Journal of Experimental Biology |
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HighWire Press (Stanford University) |
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English |
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RESEARCH ARTICLE |
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RESEARCH ARTICLE Cassidy, Alicia A. Lamarre, Simon G. Activation of oxygen-responsive pathways is associated with altered protein metabolism in Arctic char exposed to hypoxia |
topic_facet |
RESEARCH ARTICLE |
description |
Fish exposed to fluctuating oxygen concentrations often alter their metabolism and/or behaviour to survive. Hypoxia tolerance is typically associated with the ability to reduce energy demand by supressing metabolic processes such as protein synthesis. Arctic char is amongst the most sensitive salmonid to hypoxia, and typically engage in avoidance behaviour when faced with lack of oxygen. We hypothesized that a sensitive species will still have the ability (albeit reduced) to regulate molecular mechanisms during hypoxia. We investigated the tissue-specific response of protein metabolism during hypoxia. Little is known about protein degradation pathways during hypoxia in fish and we predict that protein degradation pathways are differentially regulated and play a role in the hypoxia response. We also studied the regulation of oxygen-responsive cellular signalling pathways [hypoxia inducible factor (HIF), unfolded protein response (UPR) and mTOR pathways] since most of what we know comes from studies on cancerous mammalian cell lines. Arctic char were exposed to cumulative graded hypoxia trials for 3 h at four air saturation levels (100%, 50%, 30% and 15%). The rate of protein synthesis was measured using a flooding dose technique, whereas protein degradation and signalling pathways were assessed by measuring transcripts and phosphorylation of target proteins. Protein synthesis decreased in all tissues measured (liver, muscle, gill, digestive system) except for the heart. Salmonid hearts have preferential access to oxygen through a well-developed coronary artery, therefore the heart is likely to be the last tissue to become hypoxic. Autophagy markers were upregulated in the liver, whereas protein degradation markers were downregulated in the heart during hypoxia. Further work is needed to determine the effects of a decrease in protein degradation on a hypoxic salmonid heart. Our study showed that protein metabolism in Arctic char is altered in a tissue-specific fashion during graded hypoxia, which is in ... |
format |
Text |
author |
Cassidy, Alicia A. Lamarre, Simon G. |
author_facet |
Cassidy, Alicia A. Lamarre, Simon G. |
author_sort |
Cassidy, Alicia A. |
title |
Activation of oxygen-responsive pathways is associated with altered protein metabolism in Arctic char exposed to hypoxia |
title_short |
Activation of oxygen-responsive pathways is associated with altered protein metabolism in Arctic char exposed to hypoxia |
title_full |
Activation of oxygen-responsive pathways is associated with altered protein metabolism in Arctic char exposed to hypoxia |
title_fullStr |
Activation of oxygen-responsive pathways is associated with altered protein metabolism in Arctic char exposed to hypoxia |
title_full_unstemmed |
Activation of oxygen-responsive pathways is associated with altered protein metabolism in Arctic char exposed to hypoxia |
title_sort |
activation of oxygen-responsive pathways is associated with altered protein metabolism in arctic char exposed to hypoxia |
publisher |
The Company of Biologists Ltd |
publishDate |
2019 |
url |
http://jeb.biologists.org/cgi/content/short/222/22/jeb203901 https://doi.org/10.1242/jeb.203901 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
http://jeb.biologists.org/cgi/content/short/222/22/jeb203901 http://dx.doi.org/10.1242/jeb.203901 |
op_rights |
Copyright (C) 2019, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.203901 |
container_title |
Journal of Experimental Biology |
_version_ |
1766324617455075328 |