ATP-induced reversed thermal sensitivity of O2 binding in both major haemoglobin polymorphs of the non-endothermic Atlantic cod, Gadus morhua
Atlantic cod is a species that is affected by climate change, with some populations being exposed to higher temperatures than others. The two polymorphs of its major haemoglobin type (HbI) show an inverse change in frequency along a latitudinal temperature cline in the North East Atlantic, which has...
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fthighwire:oai:open-archive.highwire.org:jexbio:222/12/jeb200279 2023-05-15T15:27:07+02:00 ATP-induced reversed thermal sensitivity of O2 binding in both major haemoglobin polymorphs of the non-endothermic Atlantic cod, Gadus morhua Nelson, Charlotte Barlow, Samantha L. Berenbrink, Michael 2019-06-21 06:58:25.0 text/html http://jeb.biologists.org/cgi/content/short/222/12/jeb200279 https://doi.org/10.1242/jeb.200279 en eng The Company of Biologists Ltd http://jeb.biologists.org/cgi/content/short/222/12/jeb200279 http://dx.doi.org/10.1242/jeb.200279 Copyright (C) 2019, Company of Biologists RESEARCH ARTICLE TEXT 2019 fthighwire https://doi.org/10.1242/jeb.200279 2019-10-03T17:08:30Z Atlantic cod is a species that is affected by climate change, with some populations being exposed to higher temperatures than others. The two polymorphs of its major haemoglobin type (HbI) show an inverse change in frequency along a latitudinal temperature cline in the North East Atlantic, which has been associated with differences in population performance and behavioural traits. An earlier study at the northern distribution limit of the species reported differential temperature sensitivities of red blood cell oxygen (O 2 ) affinity between the northern cold-water HbI-2 polymorph and its southern, warm-water HbI-1 counter-part, which has since widely been held as adaptive for the species across its distributional range. The present study critically re-examined this hypothesis by comparing the thermal sensitivity of O 2 binding in both purified HbI polymorphs from the southern, high-temperature distribution limit of the species under controlled conditions of allosteric modifiers of Hb function. Contrary to the prevailing view, the O 2 affinity of the major HbI polymorphs did not differ from each other under any of the tested conditions. Depending on pH and ATP concentration, the temperature-sensitive and temperature-insensitive Hb–O 2 affinity phenotypes – previously exclusively ascribed to HbI-1 and HbI-2, respectively – could be induced in both HbI polymorphs. These results are the first to establish a molecular mechanism behind a reversed temperature dependence of red blood cell O 2 affinity in a non-endotherm fish and lay the basis for future studies on alternative mechanisms behind the differences in distribution, performance and behavioural traits associated with the different HbI polymorphs of Atlantic cod. Text atlantic cod Gadus morhua North East Atlantic HighWire Press (Stanford University) Journal of Experimental Biology |
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RESEARCH ARTICLE |
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RESEARCH ARTICLE Nelson, Charlotte Barlow, Samantha L. Berenbrink, Michael ATP-induced reversed thermal sensitivity of O2 binding in both major haemoglobin polymorphs of the non-endothermic Atlantic cod, Gadus morhua |
topic_facet |
RESEARCH ARTICLE |
description |
Atlantic cod is a species that is affected by climate change, with some populations being exposed to higher temperatures than others. The two polymorphs of its major haemoglobin type (HbI) show an inverse change in frequency along a latitudinal temperature cline in the North East Atlantic, which has been associated with differences in population performance and behavioural traits. An earlier study at the northern distribution limit of the species reported differential temperature sensitivities of red blood cell oxygen (O 2 ) affinity between the northern cold-water HbI-2 polymorph and its southern, warm-water HbI-1 counter-part, which has since widely been held as adaptive for the species across its distributional range. The present study critically re-examined this hypothesis by comparing the thermal sensitivity of O 2 binding in both purified HbI polymorphs from the southern, high-temperature distribution limit of the species under controlled conditions of allosteric modifiers of Hb function. Contrary to the prevailing view, the O 2 affinity of the major HbI polymorphs did not differ from each other under any of the tested conditions. Depending on pH and ATP concentration, the temperature-sensitive and temperature-insensitive Hb–O 2 affinity phenotypes – previously exclusively ascribed to HbI-1 and HbI-2, respectively – could be induced in both HbI polymorphs. These results are the first to establish a molecular mechanism behind a reversed temperature dependence of red blood cell O 2 affinity in a non-endotherm fish and lay the basis for future studies on alternative mechanisms behind the differences in distribution, performance and behavioural traits associated with the different HbI polymorphs of Atlantic cod. |
format |
Text |
author |
Nelson, Charlotte Barlow, Samantha L. Berenbrink, Michael |
author_facet |
Nelson, Charlotte Barlow, Samantha L. Berenbrink, Michael |
author_sort |
Nelson, Charlotte |
title |
ATP-induced reversed thermal sensitivity of O2 binding in both major haemoglobin polymorphs of the non-endothermic Atlantic cod, Gadus morhua |
title_short |
ATP-induced reversed thermal sensitivity of O2 binding in both major haemoglobin polymorphs of the non-endothermic Atlantic cod, Gadus morhua |
title_full |
ATP-induced reversed thermal sensitivity of O2 binding in both major haemoglobin polymorphs of the non-endothermic Atlantic cod, Gadus morhua |
title_fullStr |
ATP-induced reversed thermal sensitivity of O2 binding in both major haemoglobin polymorphs of the non-endothermic Atlantic cod, Gadus morhua |
title_full_unstemmed |
ATP-induced reversed thermal sensitivity of O2 binding in both major haemoglobin polymorphs of the non-endothermic Atlantic cod, Gadus morhua |
title_sort |
atp-induced reversed thermal sensitivity of o2 binding in both major haemoglobin polymorphs of the non-endothermic atlantic cod, gadus morhua |
publisher |
The Company of Biologists Ltd |
publishDate |
2019 |
url |
http://jeb.biologists.org/cgi/content/short/222/12/jeb200279 https://doi.org/10.1242/jeb.200279 |
genre |
atlantic cod Gadus morhua North East Atlantic |
genre_facet |
atlantic cod Gadus morhua North East Atlantic |
op_relation |
http://jeb.biologists.org/cgi/content/short/222/12/jeb200279 http://dx.doi.org/10.1242/jeb.200279 |
op_rights |
Copyright (C) 2019, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.200279 |
container_title |
Journal of Experimental Biology |
_version_ |
1766357573748916224 |