Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris)
The northern elephant seal pup ( Mirounga angustirostris ) undergoes a 2–3 month post-weaning fast, during which it depends primarily on the oxidation of fatty acids to meet its energetic demands. The concentration of non-esterified fatty acids (NEFAs) increases and is associated with the developmen...
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fthighwire:oai:open-archive.highwire.org:jexbio:215/14/2455 2023-05-15T16:05:10+02:00 Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris) Viscarra, Jose Abraham Vázquez-Medina, José Pablo Rodriguez, Ruben Champagne, Cory D. Adams, Sean H. Crocker, Daniel E. Ortiz, Rudy M. 2012-07-15 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/215/14/2455 https://doi.org/10.1242/jeb.069070 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/215/14/2455 http://dx.doi.org/10.1242/jeb.069070 Copyright (C) 2012, Company of Biologists Research Articles TEXT 2012 fthighwire https://doi.org/10.1242/jeb.069070 2013-05-26T19:10:40Z The northern elephant seal pup ( Mirounga angustirostris ) undergoes a 2–3 month post-weaning fast, during which it depends primarily on the oxidation of fatty acids to meet its energetic demands. The concentration of non-esterified fatty acids (NEFAs) increases and is associated with the development of insulin resistance in late-fasted pups. Furthermore, plasma NEFA concentrations respond differentially to an intravenous glucose tolerance test (ivGTT) depending on fasting duration, suggesting that the effects of glucose on lipid metabolism are altered. However, elucidation of the lipolytic mechanisms including lipase activity during prolonged fasting in mammals is scarce. To assess the impact of fasting and glucose on the regulation of lipid metabolism, adipose tissue and plasma samples were collected before and after ivGTTs performed on early (2 weeks, N =5) and late (6–8 weeks; N =8) fasted pups. Glucose administration increased plasma triglycerides and NEFA concentrations in late-fasted seals, but not plasma glycerol. Fasting decreased basal adipose lipase activity by 50%. Fasting also increased plasma lipase activity twofold and decreased the expressions of CD36, FAS, FATP1 and PEPCK-C by 22–43% in adipose tissue. Plasma acylcarnitine profiling indicated that late-fasted seals display higher incomplete LCFA β-oxidation. Results suggest that long-term fasting induces shifts in the regulation of lipolysis and lipid metabolism associated with the onset of insulin resistance in northern elephant seal pups. Delineation of the mechanisms responsible for this shift in regulation during fasting can contribute to a more thorough understanding of the changes in lipid metabolism associated with dyslipidemia and insulin resistance in mammals. Text Elephant Seal HighWire Press (Stanford University) Journal of Experimental Biology 215 14 2455 2464 |
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Research Articles |
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Research Articles Viscarra, Jose Abraham Vázquez-Medina, José Pablo Rodriguez, Ruben Champagne, Cory D. Adams, Sean H. Crocker, Daniel E. Ortiz, Rudy M. Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris) |
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Research Articles |
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The northern elephant seal pup ( Mirounga angustirostris ) undergoes a 2–3 month post-weaning fast, during which it depends primarily on the oxidation of fatty acids to meet its energetic demands. The concentration of non-esterified fatty acids (NEFAs) increases and is associated with the development of insulin resistance in late-fasted pups. Furthermore, plasma NEFA concentrations respond differentially to an intravenous glucose tolerance test (ivGTT) depending on fasting duration, suggesting that the effects of glucose on lipid metabolism are altered. However, elucidation of the lipolytic mechanisms including lipase activity during prolonged fasting in mammals is scarce. To assess the impact of fasting and glucose on the regulation of lipid metabolism, adipose tissue and plasma samples were collected before and after ivGTTs performed on early (2 weeks, N =5) and late (6–8 weeks; N =8) fasted pups. Glucose administration increased plasma triglycerides and NEFA concentrations in late-fasted seals, but not plasma glycerol. Fasting decreased basal adipose lipase activity by 50%. Fasting also increased plasma lipase activity twofold and decreased the expressions of CD36, FAS, FATP1 and PEPCK-C by 22–43% in adipose tissue. Plasma acylcarnitine profiling indicated that late-fasted seals display higher incomplete LCFA β-oxidation. Results suggest that long-term fasting induces shifts in the regulation of lipolysis and lipid metabolism associated with the onset of insulin resistance in northern elephant seal pups. Delineation of the mechanisms responsible for this shift in regulation during fasting can contribute to a more thorough understanding of the changes in lipid metabolism associated with dyslipidemia and insulin resistance in mammals. |
format |
Text |
author |
Viscarra, Jose Abraham Vázquez-Medina, José Pablo Rodriguez, Ruben Champagne, Cory D. Adams, Sean H. Crocker, Daniel E. Ortiz, Rudy M. |
author_facet |
Viscarra, Jose Abraham Vázquez-Medina, José Pablo Rodriguez, Ruben Champagne, Cory D. Adams, Sean H. Crocker, Daniel E. Ortiz, Rudy M. |
author_sort |
Viscarra, Jose Abraham |
title |
Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris) |
title_short |
Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris) |
title_full |
Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris) |
title_fullStr |
Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris) |
title_full_unstemmed |
Decreased expression of adipose CD36 and FATP1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (Mirounga angustirostris) |
title_sort |
decreased expression of adipose cd36 and fatp1 are associated with increased plasma non-esterified fatty acids during prolonged fasting in northern elephant seal pups (mirounga angustirostris) |
publisher |
Company of Biologists |
publishDate |
2012 |
url |
http://jeb.biologists.org/cgi/content/short/215/14/2455 https://doi.org/10.1242/jeb.069070 |
genre |
Elephant Seal |
genre_facet |
Elephant Seal |
op_relation |
http://jeb.biologists.org/cgi/content/short/215/14/2455 http://dx.doi.org/10.1242/jeb.069070 |
op_rights |
Copyright (C) 2012, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.069070 |
container_title |
Journal of Experimental Biology |
container_volume |
215 |
container_issue |
14 |
container_start_page |
2455 |
op_container_end_page |
2464 |
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1766400973672022016 |