Antioxidant capacity develops with maturation in the deep-diving hooded seal
Maturation in hooded seals is characterized by the rapid development of their physiological diving capacity and is accompanied by increases in oxidant production but not oxidative damage. To test the hypothesis that the antioxidant system of hooded seals develops as they transition from a terrestria...
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fthighwire:oai:open-archive.highwire.org:jexbio:214/17/2903 2023-05-15T16:34:49+02:00 Antioxidant capacity develops with maturation in the deep-diving hooded seal Vázquez-Medina, José Pablo Soñanez-Organis, José Guadalupe Burns, Jennifer M. Zenteno-Savín, Tania Ortiz, Rudy M. 2011-09-01 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/214/17/2903 https://doi.org/10.1242/jeb.057935 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/214/17/2903 http://dx.doi.org/10.1242/jeb.057935 Copyright (C) 2011, Company of Biologists Research Articles TEXT 2011 fthighwire https://doi.org/10.1242/jeb.057935 2013-05-27T12:26:01Z Maturation in hooded seals is characterized by the rapid development of their physiological diving capacity and is accompanied by increases in oxidant production but not oxidative damage. To test the hypothesis that the antioxidant system of hooded seals develops as they transition from a terrestrial to an aquatic environment, we obtained the complete cDNA sequence that encodes the NF-E2-related factor 2 (Nrf2), a central regulator of the antioxidant response, and compared Nrf2 mRNA and protein expression levels in muscle samples from neonate, weaned pups and adult hooded seals, along with glutathione (GSH) levels and the activity/protein content of the antioxidant enzymes catalase, glutathione peroxidase (GPx), peroxyredoxin VI (PrxVI), thioredoxin 1 (Trx1), thioredoxin reductase (TrxR), glutaredoxin 1 (Glrx1), glutathione disulphide reductase, glutathione S-transferase and glutamate-cysteine ligase. The Nrf2 of the hooded seal is 1822 bp long and encodes a protein of 606 amino acids with a leucine zipper domain and Keap1-mediated proteosomal degradation residues, which are key for Nrf2 function and regulation. Although neither Nrf2 mRNA nor Nrf2 nuclear protein content are higher in adults than in pups, GSH levels along with GPx, PrxVI, Trx1, TrxR and Glrx1 activity/protein content increase with maturation, suggesting that the potential for peroxide removal increases with development in hooded seals, and that these enzymes contribute to the regulation of the intracellular redox state and the prevention of oxidative damage in these deep-diving mammals. Text hooded seal HighWire Press (Stanford University) Journal of Experimental Biology 214 17 2903 2910 |
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Research Articles |
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Research Articles Vázquez-Medina, José Pablo Soñanez-Organis, José Guadalupe Burns, Jennifer M. Zenteno-Savín, Tania Ortiz, Rudy M. Antioxidant capacity develops with maturation in the deep-diving hooded seal |
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Research Articles |
description |
Maturation in hooded seals is characterized by the rapid development of their physiological diving capacity and is accompanied by increases in oxidant production but not oxidative damage. To test the hypothesis that the antioxidant system of hooded seals develops as they transition from a terrestrial to an aquatic environment, we obtained the complete cDNA sequence that encodes the NF-E2-related factor 2 (Nrf2), a central regulator of the antioxidant response, and compared Nrf2 mRNA and protein expression levels in muscle samples from neonate, weaned pups and adult hooded seals, along with glutathione (GSH) levels and the activity/protein content of the antioxidant enzymes catalase, glutathione peroxidase (GPx), peroxyredoxin VI (PrxVI), thioredoxin 1 (Trx1), thioredoxin reductase (TrxR), glutaredoxin 1 (Glrx1), glutathione disulphide reductase, glutathione S-transferase and glutamate-cysteine ligase. The Nrf2 of the hooded seal is 1822 bp long and encodes a protein of 606 amino acids with a leucine zipper domain and Keap1-mediated proteosomal degradation residues, which are key for Nrf2 function and regulation. Although neither Nrf2 mRNA nor Nrf2 nuclear protein content are higher in adults than in pups, GSH levels along with GPx, PrxVI, Trx1, TrxR and Glrx1 activity/protein content increase with maturation, suggesting that the potential for peroxide removal increases with development in hooded seals, and that these enzymes contribute to the regulation of the intracellular redox state and the prevention of oxidative damage in these deep-diving mammals. |
format |
Text |
author |
Vázquez-Medina, José Pablo Soñanez-Organis, José Guadalupe Burns, Jennifer M. Zenteno-Savín, Tania Ortiz, Rudy M. |
author_facet |
Vázquez-Medina, José Pablo Soñanez-Organis, José Guadalupe Burns, Jennifer M. Zenteno-Savín, Tania Ortiz, Rudy M. |
author_sort |
Vázquez-Medina, José Pablo |
title |
Antioxidant capacity develops with maturation in the deep-diving hooded seal |
title_short |
Antioxidant capacity develops with maturation in the deep-diving hooded seal |
title_full |
Antioxidant capacity develops with maturation in the deep-diving hooded seal |
title_fullStr |
Antioxidant capacity develops with maturation in the deep-diving hooded seal |
title_full_unstemmed |
Antioxidant capacity develops with maturation in the deep-diving hooded seal |
title_sort |
antioxidant capacity develops with maturation in the deep-diving hooded seal |
publisher |
Company of Biologists |
publishDate |
2011 |
url |
http://jeb.biologists.org/cgi/content/short/214/17/2903 https://doi.org/10.1242/jeb.057935 |
genre |
hooded seal |
genre_facet |
hooded seal |
op_relation |
http://jeb.biologists.org/cgi/content/short/214/17/2903 http://dx.doi.org/10.1242/jeb.057935 |
op_rights |
Copyright (C) 2011, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.057935 |
container_title |
Journal of Experimental Biology |
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214 |
container_issue |
17 |
container_start_page |
2903 |
op_container_end_page |
2910 |
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1766024855219601408 |