Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions
Pesticide run-off into the ocean represents a potential threat to marine organisms, especially bivalves living in coastal environments. However, little is known about the effects of environmentally relevant concentrations of pesticides at the individual level. In this study, the suppression subtract...
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fthighwire:oai:open-archive.highwire.org:jexbio:213/23/4010 2023-05-15T15:58:02+02:00 Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions Collin, Hélène Meistertzheim, Anne-Leila David, Elise Moraga, Dario Boutet, Isabelle 2010-12-01 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/213/23/4010 https://doi.org/10.1242/jeb.048033 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/213/23/4010 http://dx.doi.org/10.1242/jeb.048033 Copyright (C) 2010, Company of Biologists Research Articles TEXT 2010 fthighwire https://doi.org/10.1242/jeb.048033 2015-02-28T21:02:37Z Pesticide run-off into the ocean represents a potential threat to marine organisms, especially bivalves living in coastal environments. However, little is known about the effects of environmentally relevant concentrations of pesticides at the individual level. In this study, the suppression subtractive hybridisation technique was used to discover the main physiological function affected by a cocktail of three pesticides (lindane, metolachlor and carbofuran) in the Pacific oyster Crassostrea gigas . Two oyster populations exposed to different pollution levels in the wild were investigated. The pesticide concentrations used to induce stress were close to those found in the wild. In a time course experiment, the expression of three genes implicated in iron metabolism and oxidative stress as well as that of two ubiquitous stress proteins was examined. No clear regulation of gene or protein expression was found, potentially due to a low-dose effect. However, we detected a strong site- and organ-specific response to the pesticides. This study thus (1) provides insight into bivalve responses to pesticide pollution at the level of the transcriptome, which is the first level of response for organisms facing pollution, and (2) raises interesting questions concerning the importance of the sites and organs studied in the toxicogenomic field. Text Crassostrea gigas Pacific oyster HighWire Press (Stanford University) Pacific Journal of Experimental Biology 213 23 4010 4017 |
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Research Articles |
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Research Articles Collin, Hélène Meistertzheim, Anne-Leila David, Elise Moraga, Dario Boutet, Isabelle Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
topic_facet |
Research Articles |
description |
Pesticide run-off into the ocean represents a potential threat to marine organisms, especially bivalves living in coastal environments. However, little is known about the effects of environmentally relevant concentrations of pesticides at the individual level. In this study, the suppression subtractive hybridisation technique was used to discover the main physiological function affected by a cocktail of three pesticides (lindane, metolachlor and carbofuran) in the Pacific oyster Crassostrea gigas . Two oyster populations exposed to different pollution levels in the wild were investigated. The pesticide concentrations used to induce stress were close to those found in the wild. In a time course experiment, the expression of three genes implicated in iron metabolism and oxidative stress as well as that of two ubiquitous stress proteins was examined. No clear regulation of gene or protein expression was found, potentially due to a low-dose effect. However, we detected a strong site- and organ-specific response to the pesticides. This study thus (1) provides insight into bivalve responses to pesticide pollution at the level of the transcriptome, which is the first level of response for organisms facing pollution, and (2) raises interesting questions concerning the importance of the sites and organs studied in the toxicogenomic field. |
format |
Text |
author |
Collin, Hélène Meistertzheim, Anne-Leila David, Elise Moraga, Dario Boutet, Isabelle |
author_facet |
Collin, Hélène Meistertzheim, Anne-Leila David, Elise Moraga, Dario Boutet, Isabelle |
author_sort |
Collin, Hélène |
title |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_short |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_full |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_fullStr |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_full_unstemmed |
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions |
title_sort |
response of the pacific oyster crassostrea gigas, thunberg 1793, to pesticide exposure under experimental conditions |
publisher |
Company of Biologists |
publishDate |
2010 |
url |
http://jeb.biologists.org/cgi/content/short/213/23/4010 https://doi.org/10.1242/jeb.048033 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://jeb.biologists.org/cgi/content/short/213/23/4010 http://dx.doi.org/10.1242/jeb.048033 |
op_rights |
Copyright (C) 2010, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.048033 |
container_title |
Journal of Experimental Biology |
container_volume |
213 |
container_issue |
23 |
container_start_page |
4010 |
op_container_end_page |
4017 |
_version_ |
1766393747264765952 |