A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue
The dermis of sea cucumbers is a catch connective tissue or mutable collagenous tissue that shows large changes in stiffness. Extensive studies on the dermis revealed that it can adopt three different states having different mechanical properties that can be reversibly converted. These are the stiff...
Published in: | Journal of Experimental Biology |
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2010
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fthighwire:oai:open-archive.highwire.org:jexbio:213/20/3416 2023-05-15T15:59:39+02:00 A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue Yamada, Akira Tamori, Masaki Iketani, Tomoaki Oiwa, Kazuhiro Motokawa, Tatsuo 2010-10-15 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/213/20/3416 https://doi.org/10.1242/jeb.044149 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/213/20/3416 http://dx.doi.org/10.1242/jeb.044149 Copyright (C) 2010, Company of Biologists Research Articles TEXT 2010 fthighwire https://doi.org/10.1242/jeb.044149 2015-02-28T21:02:31Z The dermis of sea cucumbers is a catch connective tissue or mutable collagenous tissue that shows large changes in stiffness. Extensive studies on the dermis revealed that it can adopt three different states having different mechanical properties that can be reversibly converted. These are the stiff, standard and soft states. The standard state is readily produced when a dermal piece is immersed in the sea water containing Ca2+, whereas the soft state can be produced by removal of Ca2+. A stiffening protein, tensilin, has been isolated from some sea cucumbers ( Cucumaria frondosa and Holothuria leucospilota ). Although tensilin converts the state of the dermis from soft to standard, it cannot convert from standard to stiff. In this study, we isolated and partially purified a novel stiffening factor from the dermis of Holothuria leucospilota . The factor stiffened the dermis in normal artificial sea water (ASW) but did not stiffen the soft dermis in Ca2+-free ASW. It also stiffened the dermis that had been converted to the standard state in Ca2+-free ASW by the action of tensilin. These results suggest that the factor produces the stiff dermis from the standard state but cannot work as a stiffener on the soft dermis. Its addition to longitudinal muscles of the sea cucumber produced no effects, suggesting that its effect is specific to the catch connective tissue. Its stiffening activity was susceptible to trypsin, meaning that it is a polypeptide, and its molecular mass estimated from gel filtration chromatography was 2.4 kDa. Text Cucumaria frondosa HighWire Press (Stanford University) Journal of Experimental Biology 213 20 3416 3422 |
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HighWire Press (Stanford University) |
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English |
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Research Articles |
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Research Articles Yamada, Akira Tamori, Masaki Iketani, Tomoaki Oiwa, Kazuhiro Motokawa, Tatsuo A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue |
topic_facet |
Research Articles |
description |
The dermis of sea cucumbers is a catch connective tissue or mutable collagenous tissue that shows large changes in stiffness. Extensive studies on the dermis revealed that it can adopt three different states having different mechanical properties that can be reversibly converted. These are the stiff, standard and soft states. The standard state is readily produced when a dermal piece is immersed in the sea water containing Ca2+, whereas the soft state can be produced by removal of Ca2+. A stiffening protein, tensilin, has been isolated from some sea cucumbers ( Cucumaria frondosa and Holothuria leucospilota ). Although tensilin converts the state of the dermis from soft to standard, it cannot convert from standard to stiff. In this study, we isolated and partially purified a novel stiffening factor from the dermis of Holothuria leucospilota . The factor stiffened the dermis in normal artificial sea water (ASW) but did not stiffen the soft dermis in Ca2+-free ASW. It also stiffened the dermis that had been converted to the standard state in Ca2+-free ASW by the action of tensilin. These results suggest that the factor produces the stiff dermis from the standard state but cannot work as a stiffener on the soft dermis. Its addition to longitudinal muscles of the sea cucumber produced no effects, suggesting that its effect is specific to the catch connective tissue. Its stiffening activity was susceptible to trypsin, meaning that it is a polypeptide, and its molecular mass estimated from gel filtration chromatography was 2.4 kDa. |
format |
Text |
author |
Yamada, Akira Tamori, Masaki Iketani, Tomoaki Oiwa, Kazuhiro Motokawa, Tatsuo |
author_facet |
Yamada, Akira Tamori, Masaki Iketani, Tomoaki Oiwa, Kazuhiro Motokawa, Tatsuo |
author_sort |
Yamada, Akira |
title |
A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue |
title_short |
A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue |
title_full |
A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue |
title_fullStr |
A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue |
title_full_unstemmed |
A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue |
title_sort |
novel stiffening factor inducing the stiffest state of holothurian catch connective tissue |
publisher |
Company of Biologists |
publishDate |
2010 |
url |
http://jeb.biologists.org/cgi/content/short/213/20/3416 https://doi.org/10.1242/jeb.044149 |
genre |
Cucumaria frondosa |
genre_facet |
Cucumaria frondosa |
op_relation |
http://jeb.biologists.org/cgi/content/short/213/20/3416 http://dx.doi.org/10.1242/jeb.044149 |
op_rights |
Copyright (C) 2010, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.044149 |
container_title |
Journal of Experimental Biology |
container_volume |
213 |
container_issue |
20 |
container_start_page |
3416 |
op_container_end_page |
3422 |
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1766395572889059328 |