Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias
After a meal, dogfish exhibit a metabolic alkalosis in the bloodstream and a marked excretion of basic equivalents across the gills to the external seawater. We used the H+, K+-ATPase pump inhibitor omeprazole to determine whether these post-prandial alkaline tide events were linked to secretion of...
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fthighwire:oai:open-archive.highwire.org:jexbio:212/5/684 2023-05-15T18:51:07+02:00 Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias Wood, Chris M. Schultz, Aaron G. Munger, R. Stephen Walsh, Patrick J. 2009-03-01 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/212/5/684 https://doi.org/10.1242/jeb.026450 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/212/5/684 http://dx.doi.org/10.1242/jeb.026450 Copyright (C) 2009, Company of Biologists Research Article TEXT 2009 fthighwire https://doi.org/10.1242/jeb.026450 2015-03-01T00:41:00Z After a meal, dogfish exhibit a metabolic alkalosis in the bloodstream and a marked excretion of basic equivalents across the gills to the external seawater. We used the H+, K+-ATPase pump inhibitor omeprazole to determine whether these post-prandial alkaline tide events were linked to secretion of H+ (accompanied by Cl–) in the stomach. Sharks were fitted with indwelling stomach tubes for pretreatment with omeprazole (five doses of 5 mg omeprazole per kilogram over 48 h) or comparable volumes of vehicle (saline containing 2% DMSO) and for sampling of gastric chyme. Fish were then fed an involuntary meal by means of the stomach tube consisting of minced flatfish muscle (2% of body mass) suspended in saline (4% of body mass total volume). Omeprazole pre-treatment delayed the post-prandial acidification of the gastric chyme, slowed the rise in Cl– concentration of the chyme and altered the patterns of other ions, indicating inhibition of H+ and accompanying Cl– secretion. Omeprazole also greatly attenuated the rise in arterial pH and bicarbonate concentrations and reduced the net excretion of basic equivalents to the water by 56% over 48 h. Arterial blood CO 2 pressure ( P a CO 2 ) and plasma ions were not substantially altered. These results indicate that elevated gastric H+ secretion (as HCl) in the digestive process is the major cause of the systemic metabolic alkalosis and the accompanying rise in base excretion across the gills that constitute the alkaline tide in the dogfish. Text spiny dogfish Squalus acanthias HighWire Press (Stanford University) Journal of Experimental Biology 212 5 684 692 |
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HighWire Press (Stanford University) |
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English |
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Research Article |
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Research Article Wood, Chris M. Schultz, Aaron G. Munger, R. Stephen Walsh, Patrick J. Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias |
topic_facet |
Research Article |
description |
After a meal, dogfish exhibit a metabolic alkalosis in the bloodstream and a marked excretion of basic equivalents across the gills to the external seawater. We used the H+, K+-ATPase pump inhibitor omeprazole to determine whether these post-prandial alkaline tide events were linked to secretion of H+ (accompanied by Cl–) in the stomach. Sharks were fitted with indwelling stomach tubes for pretreatment with omeprazole (five doses of 5 mg omeprazole per kilogram over 48 h) or comparable volumes of vehicle (saline containing 2% DMSO) and for sampling of gastric chyme. Fish were then fed an involuntary meal by means of the stomach tube consisting of minced flatfish muscle (2% of body mass) suspended in saline (4% of body mass total volume). Omeprazole pre-treatment delayed the post-prandial acidification of the gastric chyme, slowed the rise in Cl– concentration of the chyme and altered the patterns of other ions, indicating inhibition of H+ and accompanying Cl– secretion. Omeprazole also greatly attenuated the rise in arterial pH and bicarbonate concentrations and reduced the net excretion of basic equivalents to the water by 56% over 48 h. Arterial blood CO 2 pressure ( P a CO 2 ) and plasma ions were not substantially altered. These results indicate that elevated gastric H+ secretion (as HCl) in the digestive process is the major cause of the systemic metabolic alkalosis and the accompanying rise in base excretion across the gills that constitute the alkaline tide in the dogfish. |
format |
Text |
author |
Wood, Chris M. Schultz, Aaron G. Munger, R. Stephen Walsh, Patrick J. |
author_facet |
Wood, Chris M. Schultz, Aaron G. Munger, R. Stephen Walsh, Patrick J. |
author_sort |
Wood, Chris M. |
title |
Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias |
title_short |
Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias |
title_full |
Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias |
title_fullStr |
Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias |
title_full_unstemmed |
Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias |
title_sort |
using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, squalus acanthias |
publisher |
Company of Biologists |
publishDate |
2009 |
url |
http://jeb.biologists.org/cgi/content/short/212/5/684 https://doi.org/10.1242/jeb.026450 |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_relation |
http://jeb.biologists.org/cgi/content/short/212/5/684 http://dx.doi.org/10.1242/jeb.026450 |
op_rights |
Copyright (C) 2009, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.026450 |
container_title |
Journal of Experimental Biology |
container_volume |
212 |
container_issue |
5 |
container_start_page |
684 |
op_container_end_page |
692 |
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1766244919844798464 |