Differential expression of gill Na+,K+-ATPase {alpha}- and {beta}-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar
This study examines changes in gill Na+,K+-ATPase (NKA) α- and β-subunit isoforms, Na+,K+,2Cl- cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR I and II) in anadromous and landlocked strains of Atlantic salmon during parr-smolt transformation, and after seawater (SW...
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fthighwire:oai:open-archive.highwire.org:jexbio:210/16/2885 2023-05-15T15:32:11+02:00 Differential expression of gill Na+,K+-ATPase {alpha}- and {beta}-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar Nilsen, Tom O. Ebbesson, Lars O. E. Madsen, Steffen S. McCormick, Stephen D. Andersson, Eva Björnsson, Björn Th. Prunet, Patrick Stefansson, Sigurd O. 2007-08-15 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/210/16/2885 https://doi.org/10.1242/jeb.002873 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/210/16/2885 http://dx.doi.org/10.1242/jeb.002873 Copyright (C) 2007, Company of Biologists Research Article TEXT 2007 fthighwire https://doi.org/10.1242/jeb.002873 2015-02-28T18:21:20Z This study examines changes in gill Na+,K+-ATPase (NKA) α- and β-subunit isoforms, Na+,K+,2Cl- cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR I and II) in anadromous and landlocked strains of Atlantic salmon during parr-smolt transformation, and after seawater (SW) transfer in May/June. Gill NKA activity increased from February through April, May and June among both strains in freshwater (FW), with peak enzyme activity in the landlocked salmon being 50% below that of the anadromous fish in May and June. Gill NKA-α1b, -α3, -β 1 and NKCC mRNA levels in anadromous salmon increased transiently, reaching peak levels in smolts in April/May, whereas no similar smolt-related upregulation of these transcripts occurred in juvenile landlocked salmon. Gill NKA-α1a mRNA decreased significantly in anadromous salmon from February through June, whereas α1a levels in landlocked salmon, after an initial decrease in April, remained significantly higher than those of the anadromous smolts in May and June. Following SW transfer, gill NKA-α1b and NKCC mRNA increased in both strains, whereas NKA-α1a decreased. Both strains exhibited a transient increase in gill NKA α-protein abundance, with peak levels in May. Gill α-protein abundance was lower in SW than corresponding FW values in June. Gill NKCC protein abundance increased transiently in anadromous fish, with peak levels in May, whereas a slight increase was observed in landlocked salmon in May, increasing to peak levels in June. Gill CFTR I mRNA levels increased significantly from February to April in both strains, followed by a slight, though not significant increase in May and June. CFTR I mRNA levels were significantly lower in landlocked than anadromous salmon in April/June. Gill CFTR II mRNA levels did not change significantly in either strain. Our findings demonstrates that differential expression of gill NKA-α1a, -α1b and -α3 isoforms may be important for potential functional differences in NKA, both during preparatory development and ... Text Atlantic salmon Salmo salar HighWire Press (Stanford University) Journal of Experimental Biology 210 16 2885 2896 |
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Research Article Nilsen, Tom O. Ebbesson, Lars O. E. Madsen, Steffen S. McCormick, Stephen D. Andersson, Eva Björnsson, Björn Th. Prunet, Patrick Stefansson, Sigurd O. Differential expression of gill Na+,K+-ATPase {alpha}- and {beta}-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
topic_facet |
Research Article |
description |
This study examines changes in gill Na+,K+-ATPase (NKA) α- and β-subunit isoforms, Na+,K+,2Cl- cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR I and II) in anadromous and landlocked strains of Atlantic salmon during parr-smolt transformation, and after seawater (SW) transfer in May/June. Gill NKA activity increased from February through April, May and June among both strains in freshwater (FW), with peak enzyme activity in the landlocked salmon being 50% below that of the anadromous fish in May and June. Gill NKA-α1b, -α3, -β 1 and NKCC mRNA levels in anadromous salmon increased transiently, reaching peak levels in smolts in April/May, whereas no similar smolt-related upregulation of these transcripts occurred in juvenile landlocked salmon. Gill NKA-α1a mRNA decreased significantly in anadromous salmon from February through June, whereas α1a levels in landlocked salmon, after an initial decrease in April, remained significantly higher than those of the anadromous smolts in May and June. Following SW transfer, gill NKA-α1b and NKCC mRNA increased in both strains, whereas NKA-α1a decreased. Both strains exhibited a transient increase in gill NKA α-protein abundance, with peak levels in May. Gill α-protein abundance was lower in SW than corresponding FW values in June. Gill NKCC protein abundance increased transiently in anadromous fish, with peak levels in May, whereas a slight increase was observed in landlocked salmon in May, increasing to peak levels in June. Gill CFTR I mRNA levels increased significantly from February to April in both strains, followed by a slight, though not significant increase in May and June. CFTR I mRNA levels were significantly lower in landlocked than anadromous salmon in April/June. Gill CFTR II mRNA levels did not change significantly in either strain. Our findings demonstrates that differential expression of gill NKA-α1a, -α1b and -α3 isoforms may be important for potential functional differences in NKA, both during preparatory development and ... |
format |
Text |
author |
Nilsen, Tom O. Ebbesson, Lars O. E. Madsen, Steffen S. McCormick, Stephen D. Andersson, Eva Björnsson, Björn Th. Prunet, Patrick Stefansson, Sigurd O. |
author_facet |
Nilsen, Tom O. Ebbesson, Lars O. E. Madsen, Steffen S. McCormick, Stephen D. Andersson, Eva Björnsson, Björn Th. Prunet, Patrick Stefansson, Sigurd O. |
author_sort |
Nilsen, Tom O. |
title |
Differential expression of gill Na+,K+-ATPase {alpha}- and {beta}-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_short |
Differential expression of gill Na+,K+-ATPase {alpha}- and {beta}-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_full |
Differential expression of gill Na+,K+-ATPase {alpha}- and {beta}-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_fullStr |
Differential expression of gill Na+,K+-ATPase {alpha}- and {beta}-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_full_unstemmed |
Differential expression of gill Na+,K+-ATPase {alpha}- and {beta}-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar |
title_sort |
differential expression of gill na+,k+-atpase {alpha}- and {beta}-subunits, na+,k+,2cl- cotransporter and cftr anion channel in juvenile anadromous and landlocked atlantic salmon salmo salar |
publisher |
Company of Biologists |
publishDate |
2007 |
url |
http://jeb.biologists.org/cgi/content/short/210/16/2885 https://doi.org/10.1242/jeb.002873 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://jeb.biologists.org/cgi/content/short/210/16/2885 http://dx.doi.org/10.1242/jeb.002873 |
op_rights |
Copyright (C) 2007, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.002873 |
container_title |
Journal of Experimental Biology |
container_volume |
210 |
container_issue |
16 |
container_start_page |
2885 |
op_container_end_page |
2896 |
_version_ |
1766362697443573760 |