Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion
We have previously shown that continuous intravenous infusion of NaHCO 3 for 24 h (∼1000 μmol kg-1 h-1) results in the relocation of V-H+-ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performe...
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fthighwire:oai:open-archive.highwire.org:jexbio:209/4/599 2023-05-15T18:51:07+02:00 Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion Tresguerres, Martin Parks, Scott K. Katoh, Fumi Goss, Greg G. 2006-02-15 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/209/4/599 https://doi.org/10.1242/jeb.02059 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/209/4/599 http://dx.doi.org/10.1242/jeb.02059 Copyright (C) 2006, Company of Biologists Research Article TEXT 2006 fthighwire https://doi.org/10.1242/jeb.02059 2015-02-28T19:03:49Z We have previously shown that continuous intravenous infusion of NaHCO 3 for 24 h (∼1000 μmol kg-1 h-1) results in the relocation of V-H+-ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO 2 were significantly higher in the colchicines-treated, HCO 3 --infused fish compared with fish infused with HCO 3 - alone. The effect of colchicine was highest after 24 h of infusion (8.33±0.06 vs 8.02±0.03 pH units, 15.72±3.29 vs 6.74±1.34 mmol CO 2 l-1, N =5). Immunohistochemistry and western blotting confirmed that colchicine blocked the transit of V-H+-ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H+-ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H+-ATPase is necessary for enhanced HCO 3 - secretion across the gills of the Pacific dogfish. Text spiny dogfish Squalus acanthias HighWire Press (Stanford University) Pacific Journal of Experimental Biology 209 4 599 609 |
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HighWire Press (Stanford University) |
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English |
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Research Article |
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Research Article Tresguerres, Martin Parks, Scott K. Katoh, Fumi Goss, Greg G. Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion |
topic_facet |
Research Article |
description |
We have previously shown that continuous intravenous infusion of NaHCO 3 for 24 h (∼1000 μmol kg-1 h-1) results in the relocation of V-H+-ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO 2 were significantly higher in the colchicines-treated, HCO 3 --infused fish compared with fish infused with HCO 3 - alone. The effect of colchicine was highest after 24 h of infusion (8.33±0.06 vs 8.02±0.03 pH units, 15.72±3.29 vs 6.74±1.34 mmol CO 2 l-1, N =5). Immunohistochemistry and western blotting confirmed that colchicine blocked the transit of V-H+-ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H+-ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H+-ATPase is necessary for enhanced HCO 3 - secretion across the gills of the Pacific dogfish. |
format |
Text |
author |
Tresguerres, Martin Parks, Scott K. Katoh, Fumi Goss, Greg G. |
author_facet |
Tresguerres, Martin Parks, Scott K. Katoh, Fumi Goss, Greg G. |
author_sort |
Tresguerres, Martin |
title |
Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion |
title_short |
Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion |
title_full |
Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion |
title_fullStr |
Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion |
title_full_unstemmed |
Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion |
title_sort |
microtubule-dependent relocation of branchial v-h+-atpase to the basolateral membrane in the pacific spiny dogfish (squalus acanthias): a role in base secretion |
publisher |
Company of Biologists |
publishDate |
2006 |
url |
http://jeb.biologists.org/cgi/content/short/209/4/599 https://doi.org/10.1242/jeb.02059 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_relation |
http://jeb.biologists.org/cgi/content/short/209/4/599 http://dx.doi.org/10.1242/jeb.02059 |
op_rights |
Copyright (C) 2006, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.02059 |
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Journal of Experimental Biology |
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209 |
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599 |
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