Effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of Atlantic cod Gadus morhua
The transcript levels of three genes coding for antioxidants, Cu/Zn superoxide dismutase (SOD), catalase and phospholipid hydroperoxide glutathione peroxidase (GSH-Px), and those of two stress proteins, metallothionein (MT) and CYP1A, were examined with real-time quantitative (q) RT-PCR in hepatic t...
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fthighwire:oai:open-archive.highwire.org:jexbio:209/15/2893 2023-05-15T15:27:13+02:00 Effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of Atlantic cod Gadus morhua Olsvik, P. A. Kristensen, T. Waagbø, R. Tollefsen, K.-E. Rosseland, B. O. Toften, H. 2006-08-01 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/209/15/2893 https://doi.org/10.1242/jeb.02320 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/209/15/2893 http://dx.doi.org/10.1242/jeb.02320 Copyright (C) 2006, Company of Biologists Research Article TEXT 2006 fthighwire https://doi.org/10.1242/jeb.02320 2013-05-26T21:55:22Z The transcript levels of three genes coding for antioxidants, Cu/Zn superoxide dismutase (SOD), catalase and phospholipid hydroperoxide glutathione peroxidase (GSH-Px), and those of two stress proteins, metallothionein (MT) and CYP1A, were examined with real-time quantitative (q) RT-PCR in hepatic tissue of Atlantic cod exposed to 46% (hypoxia), 76% (normoxia) and 145% (hyperoxia) O 2 saturation (tank outlet). To evaluate the oxidative stress state, the levels of total glutathione (tGSH), reduced glutathione (GSH) and oxidized glutathione (GSSG) and subsequently the oxidative stress index (OSI), were determined in the same tissue samples. The transcript level of GSH-Px was significantly upregulated in fish exposed to hyperoxia, and significantly downregulated in fish exposed to hypoxia, compared to the normoxia group. Significant downregulation was also found for SOD and CYP1A transcriptional levels in fish exposed to hypoxia. The transcript levels of catalase and MT did not change in liver of cod exposed to suboptimal oxygen levels. No significant differences were seen between the groups for tGSH, GSH, GSSG or OSI. Prolonged exposure to unfavourable oxygen saturation levels did not alter the OSI, indicating that the antioxidant glutathione system is maintained at an unchanged level in liver of the examined cod. Text atlantic cod Gadus morhua HighWire Press (Stanford University) Journal of Experimental Biology 209 15 2893 2901 |
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HighWire Press (Stanford University) |
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English |
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Research Article |
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Research Article Olsvik, P. A. Kristensen, T. Waagbø, R. Tollefsen, K.-E. Rosseland, B. O. Toften, H. Effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of Atlantic cod Gadus morhua |
topic_facet |
Research Article |
description |
The transcript levels of three genes coding for antioxidants, Cu/Zn superoxide dismutase (SOD), catalase and phospholipid hydroperoxide glutathione peroxidase (GSH-Px), and those of two stress proteins, metallothionein (MT) and CYP1A, were examined with real-time quantitative (q) RT-PCR in hepatic tissue of Atlantic cod exposed to 46% (hypoxia), 76% (normoxia) and 145% (hyperoxia) O 2 saturation (tank outlet). To evaluate the oxidative stress state, the levels of total glutathione (tGSH), reduced glutathione (GSH) and oxidized glutathione (GSSG) and subsequently the oxidative stress index (OSI), were determined in the same tissue samples. The transcript level of GSH-Px was significantly upregulated in fish exposed to hyperoxia, and significantly downregulated in fish exposed to hypoxia, compared to the normoxia group. Significant downregulation was also found for SOD and CYP1A transcriptional levels in fish exposed to hypoxia. The transcript levels of catalase and MT did not change in liver of cod exposed to suboptimal oxygen levels. No significant differences were seen between the groups for tGSH, GSH, GSSG or OSI. Prolonged exposure to unfavourable oxygen saturation levels did not alter the OSI, indicating that the antioxidant glutathione system is maintained at an unchanged level in liver of the examined cod. |
format |
Text |
author |
Olsvik, P. A. Kristensen, T. Waagbø, R. Tollefsen, K.-E. Rosseland, B. O. Toften, H. |
author_facet |
Olsvik, P. A. Kristensen, T. Waagbø, R. Tollefsen, K.-E. Rosseland, B. O. Toften, H. |
author_sort |
Olsvik, P. A. |
title |
Effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of Atlantic cod Gadus morhua |
title_short |
Effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of Atlantic cod Gadus morhua |
title_full |
Effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of Atlantic cod Gadus morhua |
title_fullStr |
Effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of Atlantic cod Gadus morhua |
title_full_unstemmed |
Effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of Atlantic cod Gadus morhua |
title_sort |
effects of hypo- and hyperoxia on transcription levels of five stress genes and the glutathione system in liver of atlantic cod gadus morhua |
publisher |
Company of Biologists |
publishDate |
2006 |
url |
http://jeb.biologists.org/cgi/content/short/209/15/2893 https://doi.org/10.1242/jeb.02320 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
http://jeb.biologists.org/cgi/content/short/209/15/2893 http://dx.doi.org/10.1242/jeb.02320 |
op_rights |
Copyright (C) 2006, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.02320 |
container_title |
Journal of Experimental Biology |
container_volume |
209 |
container_issue |
15 |
container_start_page |
2893 |
op_container_end_page |
2901 |
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1766357668674404352 |