Antifreeze activity in the gastrointestinal fluids of Arctogadus glacialis (Peters 1874) is dependent on food type
The influence of two food types, Boreogadus saida (Bs) and crustaceans (Cr), on the osmolality, ion concentrations, antifreeze activity and antifreeze glycoprotein (AFGP) distribution in the gastrointestinal fluids of the Arctic gadoid Arctogadus glacialis was determined. The gastrointestinal fluids...
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fthighwire:oai:open-archive.highwire.org:jexbio:208/13/2609 2023-05-15T15:07:20+02:00 Antifreeze activity in the gastrointestinal fluids of Arctogadus glacialis (Peters 1874) is dependent on food type Præbel, Kim Ramløv, Hans 2005-07-01 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/208/13/2609 https://doi.org/10.1242/jeb.01666 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/208/13/2609 http://dx.doi.org/10.1242/jeb.01666 Copyright (C) 2005, Company of Biologists Research Article TEXT 2005 fthighwire https://doi.org/10.1242/jeb.01666 2013-04-02T07:21:21Z The influence of two food types, Boreogadus saida (Bs) and crustaceans (Cr), on the osmolality, ion concentrations, antifreeze activity and antifreeze glycoprotein (AFGP) distribution in the gastrointestinal fluids of the Arctic gadoid Arctogadus glacialis was determined. The gastrointestinal fluids were hyperosmotic to serum but no significant differences in osmolality were found between the two food types. The food type significantly affected the antifreeze activity of the mid-gut fluids. The hysteresis freezing points, –3.27±0.30°C and –2.44±0.11°C for B. saida and crustaceans, respectively, were significantly lower than that of serum (–1.99±0.07°C). Furthermore, an exceptionally large thermal hysteresis ranging from 1.47±0.19°C to 2.04±0.30°C was observed in the intestinal fluids of fish feeding on B. saida . Native gel electrophoresis revealed that the gastrointestinal fluids contained AFGPs in all the different size groups. However, differences in band intensities for the two food types suggest that the ingested food has an influence on the concentration of the different AFGP-sizes in these fluids. A decrease in band intensities combined with a drop in thermal hysteresis from mid-gut to hind-gut fluid suggests that absorption of AFGP or possibly degradation occur during digestion. Text Arctic Arctogadus glacialis Boreogadus saida HighWire Press (Stanford University) Arctic Journal of Experimental Biology 208 13 2609 2613 |
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HighWire Press (Stanford University) |
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English |
topic |
Research Article |
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Research Article Præbel, Kim Ramløv, Hans Antifreeze activity in the gastrointestinal fluids of Arctogadus glacialis (Peters 1874) is dependent on food type |
topic_facet |
Research Article |
description |
The influence of two food types, Boreogadus saida (Bs) and crustaceans (Cr), on the osmolality, ion concentrations, antifreeze activity and antifreeze glycoprotein (AFGP) distribution in the gastrointestinal fluids of the Arctic gadoid Arctogadus glacialis was determined. The gastrointestinal fluids were hyperosmotic to serum but no significant differences in osmolality were found between the two food types. The food type significantly affected the antifreeze activity of the mid-gut fluids. The hysteresis freezing points, –3.27±0.30°C and –2.44±0.11°C for B. saida and crustaceans, respectively, were significantly lower than that of serum (–1.99±0.07°C). Furthermore, an exceptionally large thermal hysteresis ranging from 1.47±0.19°C to 2.04±0.30°C was observed in the intestinal fluids of fish feeding on B. saida . Native gel electrophoresis revealed that the gastrointestinal fluids contained AFGPs in all the different size groups. However, differences in band intensities for the two food types suggest that the ingested food has an influence on the concentration of the different AFGP-sizes in these fluids. A decrease in band intensities combined with a drop in thermal hysteresis from mid-gut to hind-gut fluid suggests that absorption of AFGP or possibly degradation occur during digestion. |
format |
Text |
author |
Præbel, Kim Ramløv, Hans |
author_facet |
Præbel, Kim Ramløv, Hans |
author_sort |
Præbel, Kim |
title |
Antifreeze activity in the gastrointestinal fluids of Arctogadus glacialis (Peters 1874) is dependent on food type |
title_short |
Antifreeze activity in the gastrointestinal fluids of Arctogadus glacialis (Peters 1874) is dependent on food type |
title_full |
Antifreeze activity in the gastrointestinal fluids of Arctogadus glacialis (Peters 1874) is dependent on food type |
title_fullStr |
Antifreeze activity in the gastrointestinal fluids of Arctogadus glacialis (Peters 1874) is dependent on food type |
title_full_unstemmed |
Antifreeze activity in the gastrointestinal fluids of Arctogadus glacialis (Peters 1874) is dependent on food type |
title_sort |
antifreeze activity in the gastrointestinal fluids of arctogadus glacialis (peters 1874) is dependent on food type |
publisher |
Company of Biologists |
publishDate |
2005 |
url |
http://jeb.biologists.org/cgi/content/short/208/13/2609 https://doi.org/10.1242/jeb.01666 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctogadus glacialis Boreogadus saida |
genre_facet |
Arctic Arctogadus glacialis Boreogadus saida |
op_relation |
http://jeb.biologists.org/cgi/content/short/208/13/2609 http://dx.doi.org/10.1242/jeb.01666 |
op_rights |
Copyright (C) 2005, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.01666 |
container_title |
Journal of Experimental Biology |
container_volume |
208 |
container_issue |
13 |
container_start_page |
2609 |
op_container_end_page |
2613 |
_version_ |
1766338857532391424 |