Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing
Although general models of the processes involved in insect survival of freezing exist, there have been few studies directly investigating physiological processes during cooling, freezing and thawing, without which these models remain hypothetical. Here, we use open-flow respirometry to investigate...
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fthighwire:oai:open-archive.highwire.org:jexbio:207/8/1287 2023-05-15T13:55:31+02:00 Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing Sinclair, Brent J. Klok, C. Jaco Chown, Steven L. 2004-03-15 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/207/8/1287 https://doi.org/10.1242/jeb.00880 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/207/8/1287 http://dx.doi.org/10.1242/jeb.00880 Copyright (C) 2004, Company of Biologists Research Article TEXT 2004 fthighwire https://doi.org/10.1242/jeb.00880 2015-02-28T19:55:49Z Although general models of the processes involved in insect survival of freezing exist, there have been few studies directly investigating physiological processes during cooling, freezing and thawing, without which these models remain hypothetical. Here, we use open-flow respirometry to investigate the metabolism of the freeze-tolerant sub-Antarctic caterpillar Pringleophaga marioni Viette (Lepidoptera: Tineidae) during cooling, freezing and thawing and to compare animals exposed to non-lethal (–5.8°C) and lethal (–6.0°C, after which caterpillars are moribund for several days, and –18°C, after which caterpillars are completely unresponsive) freezing stress. We found a large decrease in metabolic rate (that is not associated with freezing) at –0.6±0.1°C and calculated a Q 10 of 2.14×103 at this breakpoint. This breakpoint is coincident with the critical thermal minimum ( CT min ) and is hypothesised to be a metabolic manifestation of the latter, possibly a failure of the Na+/K+-ATPase pump. This provides a plausible link between processes at the cellular level and observations of the action of the CT min at tissue and whole-organism levels. Caterpillars froze at –4.6±0.1°C and had detectable metabolism when frozen. Post-thaw, metabolic rates were lower than pre-freezing measurements. Post-thaw metabolic rates did not differ between temperatures that did and did not kill the caterpillars, which suggests that mortality may be a result of a breakdown in processes at the organismal, rather than cellular, level of organisation. Text Antarc* Antarctic HighWire Press (Stanford University) Antarctic Journal of Experimental Biology 207 8 1287 1294 |
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English |
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Research Article Sinclair, Brent J. Klok, C. Jaco Chown, Steven L. Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
topic_facet |
Research Article |
description |
Although general models of the processes involved in insect survival of freezing exist, there have been few studies directly investigating physiological processes during cooling, freezing and thawing, without which these models remain hypothetical. Here, we use open-flow respirometry to investigate the metabolism of the freeze-tolerant sub-Antarctic caterpillar Pringleophaga marioni Viette (Lepidoptera: Tineidae) during cooling, freezing and thawing and to compare animals exposed to non-lethal (–5.8°C) and lethal (–6.0°C, after which caterpillars are moribund for several days, and –18°C, after which caterpillars are completely unresponsive) freezing stress. We found a large decrease in metabolic rate (that is not associated with freezing) at –0.6±0.1°C and calculated a Q 10 of 2.14×103 at this breakpoint. This breakpoint is coincident with the critical thermal minimum ( CT min ) and is hypothesised to be a metabolic manifestation of the latter, possibly a failure of the Na+/K+-ATPase pump. This provides a plausible link between processes at the cellular level and observations of the action of the CT min at tissue and whole-organism levels. Caterpillars froze at –4.6±0.1°C and had detectable metabolism when frozen. Post-thaw, metabolic rates were lower than pre-freezing measurements. Post-thaw metabolic rates did not differ between temperatures that did and did not kill the caterpillars, which suggests that mortality may be a result of a breakdown in processes at the organismal, rather than cellular, level of organisation. |
format |
Text |
author |
Sinclair, Brent J. Klok, C. Jaco Chown, Steven L. |
author_facet |
Sinclair, Brent J. Klok, C. Jaco Chown, Steven L. |
author_sort |
Sinclair, Brent J. |
title |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_short |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_full |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_fullStr |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_full_unstemmed |
Metabolism of the sub-Antarctic caterpillar Pringleophaga marioni during cooling, freezing and thawing |
title_sort |
metabolism of the sub-antarctic caterpillar pringleophaga marioni during cooling, freezing and thawing |
publisher |
Company of Biologists |
publishDate |
2004 |
url |
http://jeb.biologists.org/cgi/content/short/207/8/1287 https://doi.org/10.1242/jeb.00880 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://jeb.biologists.org/cgi/content/short/207/8/1287 http://dx.doi.org/10.1242/jeb.00880 |
op_rights |
Copyright (C) 2004, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.00880 |
container_title |
Journal of Experimental Biology |
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207 |
container_issue |
8 |
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1287 |
op_container_end_page |
1294 |
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1766262180577017856 |