Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii
The Antarctic fishes, isolated over evolutionary history in the sub-zero waters of the Southern Ocean, are an ideal group for studying the processes of cold adaptation. One species of Antarctic notothenioid fish, Trematomus bernacchii , has lost the ability to induce heat shock proteins (Hsps) in re...
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fthighwire:oai:open-archive.highwire.org:jexbio:207/21/3649 2023-05-15T14:03:03+02:00 Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii Buckley, Bradley A. Place, Sean P. Hofmann, Gretchen E. 2004-10-01 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/207/21/3649 https://doi.org/10.1242/jeb.01219 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/207/21/3649 http://dx.doi.org/10.1242/jeb.01219 Copyright (C) 2004, Company of Biologists Research Article TEXT 2004 fthighwire https://doi.org/10.1242/jeb.01219 2015-02-28T21:59:06Z The Antarctic fishes, isolated over evolutionary history in the sub-zero waters of the Southern Ocean, are an ideal group for studying the processes of cold adaptation. One species of Antarctic notothenioid fish, Trematomus bernacchii , has lost the ability to induce heat shock proteins (Hsps) in response to exposure to acute thermal stress or to the toxic heavy metal cadmium, an important part of the cellular defense response to such stressors. To elucidate the mechanism responsible for the lack of Hsp induction, we examined several stages of the hsp gene expression pathway, including transcription factor activity, Hsp70 mRNA production and protein synthesis patterns, in hepatocytes from T. bernacchii . Hsp70 mRNA was detected, as was heat shock factor 1 (HSF1) with DNA-binding activity. However, exposure to elevated temperature and to chemical inducers of the heat shock response failed to increase Hsp70 mRNA levels, HSF1 activity or the concentration of any size class of Hsps. These results suggest that Hsps, inducible in nearly every other species, are expressed constitutively in the cold-adapted T. bernacchii . Text Antarc* Antarctic Southern Ocean HighWire Press (Stanford University) Antarctic Southern Ocean The Antarctic Journal of Experimental Biology 207 21 3649 3656 |
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HighWire Press (Stanford University) |
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English |
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Research Article |
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Research Article Buckley, Bradley A. Place, Sean P. Hofmann, Gretchen E. Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii |
topic_facet |
Research Article |
description |
The Antarctic fishes, isolated over evolutionary history in the sub-zero waters of the Southern Ocean, are an ideal group for studying the processes of cold adaptation. One species of Antarctic notothenioid fish, Trematomus bernacchii , has lost the ability to induce heat shock proteins (Hsps) in response to exposure to acute thermal stress or to the toxic heavy metal cadmium, an important part of the cellular defense response to such stressors. To elucidate the mechanism responsible for the lack of Hsp induction, we examined several stages of the hsp gene expression pathway, including transcription factor activity, Hsp70 mRNA production and protein synthesis patterns, in hepatocytes from T. bernacchii . Hsp70 mRNA was detected, as was heat shock factor 1 (HSF1) with DNA-binding activity. However, exposure to elevated temperature and to chemical inducers of the heat shock response failed to increase Hsp70 mRNA levels, HSF1 activity or the concentration of any size class of Hsps. These results suggest that Hsps, inducible in nearly every other species, are expressed constitutively in the cold-adapted T. bernacchii . |
format |
Text |
author |
Buckley, Bradley A. Place, Sean P. Hofmann, Gretchen E. |
author_facet |
Buckley, Bradley A. Place, Sean P. Hofmann, Gretchen E. |
author_sort |
Buckley, Bradley A. |
title |
Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii |
title_short |
Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii |
title_full |
Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii |
title_fullStr |
Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii |
title_full_unstemmed |
Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii |
title_sort |
regulation of heat shock genes in isolated hepatocytes from an antarctic fish, trematomus bernacchii |
publisher |
Company of Biologists |
publishDate |
2004 |
url |
http://jeb.biologists.org/cgi/content/short/207/21/3649 https://doi.org/10.1242/jeb.01219 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
http://jeb.biologists.org/cgi/content/short/207/21/3649 http://dx.doi.org/10.1242/jeb.01219 |
op_rights |
Copyright (C) 2004, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.01219 |
container_title |
Journal of Experimental Biology |
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207 |
container_issue |
21 |
container_start_page |
3649 |
op_container_end_page |
3656 |
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1766273532310847488 |