Influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and Antarctic fish
The ability to heal superficial wounds is an important element in an organism's repertoire of adaptive responses to environmental stress. In fish, motile cells termed keratocytes are thought to play important roles in the wound-healing process. Keratocyte motility, like other physiological rate...
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fthighwire:oai:open-archive.highwire.org:jexbio:206/24/4539 2023-05-15T14:02:08+02:00 Influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and Antarctic fish Ream, Rachael A. Theriot, Julie A. Somero, George N. 2003-12-15 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/206/24/4539 https://doi.org/10.1242/jeb.00706 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/206/24/4539 http://dx.doi.org/10.1242/jeb.00706 Copyright (C) 2003, Company of Biologists Research Article TEXT 2003 fthighwire https://doi.org/10.1242/jeb.00706 2015-02-28T16:30:10Z The ability to heal superficial wounds is an important element in an organism's repertoire of adaptive responses to environmental stress. In fish, motile cells termed keratocytes are thought to play important roles in the wound-healing process. Keratocyte motility, like other physiological rate processes, is likely to be dependent on temperature and to show adaptive variation among differently thermally adapted species. We have quantified the effects of acute temperature change and thermal acclimation on actin-based keratocyte movement in primary cultures of keratocytes from four species of teleost fish adapted to widely different thermal conditions: two eurythermal species, the longjaw mudsucker Gillichthys mirabilis (environmental temperature range of approximately 10-37°C) and a desert pupfish, Cyprinodon salinus (10-40°C), and two species from stable thermal environments, an Antarctic notothenioid, Trematomus bernacchii (-1.86°C), and a tropical clownfish, Amphiprion percula (26-30°C). For all species, keratocyte speed increased with increasing temperature. G. mirabilis and C. salinus keratocytes reached maximal speeds at 25°C and 35°C, respectively, temperatures within the species' normal thermal ranges. Keratocytes of the stenothermal species continued to increase in speed as temperature increased above the species' normal temperature ranges. The thermal limits of keratocyte motility appear to exceed those of whole-organism thermal tolerance, notably in the case of T. bernacchii . Keratocytes of T. bernacchii survived supercooling to -6°C and retained motility at temperatures as high as 20°C. Mean keratocyte speed was conserved at physiological temperatures for the three temperate and tropical species, which suggests that a certain rate of motility is advantageous for wound healing. However, there was no temperature compensation in speed of movement for keratocytes of the Antarctic fish, which have extremely slow rates of movement at physiological temperatures. Keratocytes from all species moved in a ... Text Antarc* Antarctic HighWire Press (Stanford University) Antarctic The Antarctic Journal of Experimental Biology 206 24 4539 4551 |
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HighWire Press (Stanford University) |
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English |
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Research Article |
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Research Article Ream, Rachael A. Theriot, Julie A. Somero, George N. Influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and Antarctic fish |
topic_facet |
Research Article |
description |
The ability to heal superficial wounds is an important element in an organism's repertoire of adaptive responses to environmental stress. In fish, motile cells termed keratocytes are thought to play important roles in the wound-healing process. Keratocyte motility, like other physiological rate processes, is likely to be dependent on temperature and to show adaptive variation among differently thermally adapted species. We have quantified the effects of acute temperature change and thermal acclimation on actin-based keratocyte movement in primary cultures of keratocytes from four species of teleost fish adapted to widely different thermal conditions: two eurythermal species, the longjaw mudsucker Gillichthys mirabilis (environmental temperature range of approximately 10-37°C) and a desert pupfish, Cyprinodon salinus (10-40°C), and two species from stable thermal environments, an Antarctic notothenioid, Trematomus bernacchii (-1.86°C), and a tropical clownfish, Amphiprion percula (26-30°C). For all species, keratocyte speed increased with increasing temperature. G. mirabilis and C. salinus keratocytes reached maximal speeds at 25°C and 35°C, respectively, temperatures within the species' normal thermal ranges. Keratocytes of the stenothermal species continued to increase in speed as temperature increased above the species' normal temperature ranges. The thermal limits of keratocyte motility appear to exceed those of whole-organism thermal tolerance, notably in the case of T. bernacchii . Keratocytes of T. bernacchii survived supercooling to -6°C and retained motility at temperatures as high as 20°C. Mean keratocyte speed was conserved at physiological temperatures for the three temperate and tropical species, which suggests that a certain rate of motility is advantageous for wound healing. However, there was no temperature compensation in speed of movement for keratocytes of the Antarctic fish, which have extremely slow rates of movement at physiological temperatures. Keratocytes from all species moved in a ... |
format |
Text |
author |
Ream, Rachael A. Theriot, Julie A. Somero, George N. |
author_facet |
Ream, Rachael A. Theriot, Julie A. Somero, George N. |
author_sort |
Ream, Rachael A. |
title |
Influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and Antarctic fish |
title_short |
Influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and Antarctic fish |
title_full |
Influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and Antarctic fish |
title_fullStr |
Influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and Antarctic fish |
title_full_unstemmed |
Influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and Antarctic fish |
title_sort |
influences of thermal acclimation and acute temperature change on the motility of epithelial wound-healing cells (keratocytes) of tropical, temperate and antarctic fish |
publisher |
Company of Biologists |
publishDate |
2003 |
url |
http://jeb.biologists.org/cgi/content/short/206/24/4539 https://doi.org/10.1242/jeb.00706 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://jeb.biologists.org/cgi/content/short/206/24/4539 http://dx.doi.org/10.1242/jeb.00706 |
op_rights |
Copyright (C) 2003, Company of Biologists |
op_doi |
https://doi.org/10.1242/jeb.00706 |
container_title |
Journal of Experimental Biology |
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206 |
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24 |
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4539 |
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4551 |
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1766272215881351168 |