Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla
Gas gland cells of the European eel ( Anguilla anguilla ) were cultured on collagen-coated coverslips, and intracellular pH was measured using the pH-sensitive fluorescent probe 2′,7′-bis-(2-carboxypropyl)-5-(6)-carboxyfluorescein (BCPCF). The contributions of various proton-translocating mechanisms...
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fthighwire:oai:open-archive.highwire.org:jexbio:205/8/1069 2023-05-15T13:27:24+02:00 Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla Sötz, E. Niederstätter, H. Pelster, B. 2002-04-15 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/205/8/1069 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/205/8/1069 Copyright (C) 2002, Company of Biologists Research Article TEXT 2002 fthighwire 2015-02-28T20:02:26Z Gas gland cells of the European eel ( Anguilla anguilla ) were cultured on collagen-coated coverslips, and intracellular pH was measured using the pH-sensitive fluorescent probe 2′,7′-bis-(2-carboxypropyl)-5-(6)-carboxyfluorescein (BCPCF). The contributions of various proton-translocating mechanisms to homeostasis of intracellular pH (pHi) were assessed by adding specific inhibitors of the various proton-translocating mechanisms at a constant extracellular pH (pHe) of 7.4 and after artificial acidification of the cells using the ammonium pulse technique. The greatest decrease in pHi was observed after addition of 5-( N -ethyl- N -isobutyl)-amiloride (MIA), an inhibitor of Na+/H+ exchange. Na+/H+ exchange was active under steady-state conditions at an extracellular pH of 7.4, and activity increased after intracellular acidification. Incubation of gas gland cells with 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS), an inhibitor of anion exchange, also caused a decrease in pHi, but this decrease was not as pronounced as in the presence of MIA. Furthermore, at low pHi, the effect of DIDS was further reduced, suggesting that bicarbonate-exchanging mechanisms are involved in maintaining a steady-state pHi but that their importance is reduced at low pH. Bafilomycin A 1 , a specific inhibitor of the V-ATPase, had no effect on steady-state pHi. However, recovery of intracellular pH after an artificial acid load was significantly impaired in the presence of bafilomycin. Our results suggest that Na+/H+ exchange and anion exchange are important for the regulation of pHi at alkaline values of pHe. When pHi is low, a situation probably often encountered by gas gland cells during gas secretion, Na+/H+ exchange continues to play an important role in acid secretion and a V-ATPase appears to contribute to proton secretion. Text Anguilla anguilla HighWire Press (Stanford University) |
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English |
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Research Article Sötz, E. Niederstätter, H. Pelster, B. Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla |
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Research Article |
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Gas gland cells of the European eel ( Anguilla anguilla ) were cultured on collagen-coated coverslips, and intracellular pH was measured using the pH-sensitive fluorescent probe 2′,7′-bis-(2-carboxypropyl)-5-(6)-carboxyfluorescein (BCPCF). The contributions of various proton-translocating mechanisms to homeostasis of intracellular pH (pHi) were assessed by adding specific inhibitors of the various proton-translocating mechanisms at a constant extracellular pH (pHe) of 7.4 and after artificial acidification of the cells using the ammonium pulse technique. The greatest decrease in pHi was observed after addition of 5-( N -ethyl- N -isobutyl)-amiloride (MIA), an inhibitor of Na+/H+ exchange. Na+/H+ exchange was active under steady-state conditions at an extracellular pH of 7.4, and activity increased after intracellular acidification. Incubation of gas gland cells with 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid (DIDS), an inhibitor of anion exchange, also caused a decrease in pHi, but this decrease was not as pronounced as in the presence of MIA. Furthermore, at low pHi, the effect of DIDS was further reduced, suggesting that bicarbonate-exchanging mechanisms are involved in maintaining a steady-state pHi but that their importance is reduced at low pH. Bafilomycin A 1 , a specific inhibitor of the V-ATPase, had no effect on steady-state pHi. However, recovery of intracellular pH after an artificial acid load was significantly impaired in the presence of bafilomycin. Our results suggest that Na+/H+ exchange and anion exchange are important for the regulation of pHi at alkaline values of pHe. When pHi is low, a situation probably often encountered by gas gland cells during gas secretion, Na+/H+ exchange continues to play an important role in acid secretion and a V-ATPase appears to contribute to proton secretion. |
format |
Text |
author |
Sötz, E. Niederstätter, H. Pelster, B. |
author_facet |
Sötz, E. Niederstätter, H. Pelster, B. |
author_sort |
Sötz, E. |
title |
Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla |
title_short |
Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla |
title_full |
Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla |
title_fullStr |
Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla |
title_full_unstemmed |
Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla |
title_sort |
determinants of intracellular ph in gas gland cells of the swimbladder of the european eel anguilla anguilla |
publisher |
Company of Biologists |
publishDate |
2002 |
url |
http://jeb.biologists.org/cgi/content/short/205/8/1069 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
http://jeb.biologists.org/cgi/content/short/205/8/1069 |
op_rights |
Copyright (C) 2002, Company of Biologists |
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