Carbonic anhydrase activity in tissues of the icefish Chionodraco hamatus and of the red-blooded teleosts Trematomus bernacchii and Anguilla anguilla
Carbonic anhydrase (CA) activity was measured in blood, intestine, kidney and gill of two Antarctic teleosts, the haemoglobinless Chionodraco hamatus and the red-blooded Trematomus bernacchii , and of the temperate teleost Anguilla anguilla . In all species, the highest CA activity was in the gills,...
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fthighwire:oai:open-archive.highwire.org:jexbio:204/22/3983 2023-05-15T13:27:16+02:00 Carbonic anhydrase activity in tissues of the icefish Chionodraco hamatus and of the red-blooded teleosts Trematomus bernacchii and Anguilla anguilla Maffia, M. Rizzello, A. Acierno, R. Rollo, M. Chiloiro, R. Storelli, C. 2001-11-15 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/204/22/3983 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/204/22/3983 Copyright (C) 2001, Company of Biologists Research Articles TEXT 2001 fthighwire 2013-05-27T04:29:38Z Carbonic anhydrase (CA) activity was measured in blood, intestine, kidney and gill of two Antarctic teleosts, the haemoglobinless Chionodraco hamatus and the red-blooded Trematomus bernacchii , and of the temperate teleost Anguilla anguilla . In all species, the highest CA activity was in the gills, with the greatest activity in C. hamatus . CA activity in the blood was highest in A. anguilla, but none was detected in the blood of C. hamatus despite the presence of plasma CA inhibitors. The enzyme was present but its activity was low in the intestine and kidney of all three species. The existence of very high CA activity in C. hamatus gills compared with the red-blooded species was investigated further by isolating and characterising the branchial cytosolic CA isoforms. The turnover rate of the C. hamatus isoform was significantly higher than that of T. bernacchii and A. anguilla . The isoforms from both the Antarctic species exhibited lower apparent K m ( K m,app ) and heat stability than those from A. anguilla . Sensitivity to sulphonamides was similar in all species and was within the range of the mammalian CA II isoform. The branchial CA isoforms of C. hamatus, T. bernacchii and A. anguilla displayed relative molecular masses of 28.9, 29.9 and 31.2 kDa, respectively. The results suggest that the hemoglobinless teleost possesses a different branchial cytosolic CA isoform from that of red-blooded teleosts. Text Anguilla anguilla Antarc* Antarctic Icefish HighWire Press (Stanford University) Antarctic The Antarctic |
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Research Articles |
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Research Articles Maffia, M. Rizzello, A. Acierno, R. Rollo, M. Chiloiro, R. Storelli, C. Carbonic anhydrase activity in tissues of the icefish Chionodraco hamatus and of the red-blooded teleosts Trematomus bernacchii and Anguilla anguilla |
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Research Articles |
description |
Carbonic anhydrase (CA) activity was measured in blood, intestine, kidney and gill of two Antarctic teleosts, the haemoglobinless Chionodraco hamatus and the red-blooded Trematomus bernacchii , and of the temperate teleost Anguilla anguilla . In all species, the highest CA activity was in the gills, with the greatest activity in C. hamatus . CA activity in the blood was highest in A. anguilla, but none was detected in the blood of C. hamatus despite the presence of plasma CA inhibitors. The enzyme was present but its activity was low in the intestine and kidney of all three species. The existence of very high CA activity in C. hamatus gills compared with the red-blooded species was investigated further by isolating and characterising the branchial cytosolic CA isoforms. The turnover rate of the C. hamatus isoform was significantly higher than that of T. bernacchii and A. anguilla . The isoforms from both the Antarctic species exhibited lower apparent K m ( K m,app ) and heat stability than those from A. anguilla . Sensitivity to sulphonamides was similar in all species and was within the range of the mammalian CA II isoform. The branchial CA isoforms of C. hamatus, T. bernacchii and A. anguilla displayed relative molecular masses of 28.9, 29.9 and 31.2 kDa, respectively. The results suggest that the hemoglobinless teleost possesses a different branchial cytosolic CA isoform from that of red-blooded teleosts. |
format |
Text |
author |
Maffia, M. Rizzello, A. Acierno, R. Rollo, M. Chiloiro, R. Storelli, C. |
author_facet |
Maffia, M. Rizzello, A. Acierno, R. Rollo, M. Chiloiro, R. Storelli, C. |
author_sort |
Maffia, M. |
title |
Carbonic anhydrase activity in tissues of the icefish Chionodraco hamatus and of the red-blooded teleosts Trematomus bernacchii and Anguilla anguilla |
title_short |
Carbonic anhydrase activity in tissues of the icefish Chionodraco hamatus and of the red-blooded teleosts Trematomus bernacchii and Anguilla anguilla |
title_full |
Carbonic anhydrase activity in tissues of the icefish Chionodraco hamatus and of the red-blooded teleosts Trematomus bernacchii and Anguilla anguilla |
title_fullStr |
Carbonic anhydrase activity in tissues of the icefish Chionodraco hamatus and of the red-blooded teleosts Trematomus bernacchii and Anguilla anguilla |
title_full_unstemmed |
Carbonic anhydrase activity in tissues of the icefish Chionodraco hamatus and of the red-blooded teleosts Trematomus bernacchii and Anguilla anguilla |
title_sort |
carbonic anhydrase activity in tissues of the icefish chionodraco hamatus and of the red-blooded teleosts trematomus bernacchii and anguilla anguilla |
publisher |
Company of Biologists |
publishDate |
2001 |
url |
http://jeb.biologists.org/cgi/content/short/204/22/3983 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Anguilla anguilla Antarc* Antarctic Icefish |
genre_facet |
Anguilla anguilla Antarc* Antarctic Icefish |
op_relation |
http://jeb.biologists.org/cgi/content/short/204/22/3983 |
op_rights |
Copyright (C) 2001, Company of Biologists |
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1766397430912253952 |