Kinetics of Calcium Fluxes Across the Intestinal Mucosa of the Marine Teleost, Gadus Morhua, Measured Using an In Vitro Perfusion Method
An in vitro technique for perfusion of the intestinal vasculature and lumen was developed and used to measure calcium (Ca2+) fluxes across the intestinal mucosa of the marine teleost, the Atlantic cod ( Gadus morhua ). Saturable and nonsaturable components of the calcium influx and efflux were deter...
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fthighwire:oai:open-archive.highwire.org:jexbio:140/1/171 2023-05-15T15:27:11+02:00 Kinetics of Calcium Fluxes Across the Intestinal Mucosa of the Marine Teleost, Gadus Morhua, Measured Using an In Vitro Perfusion Method SUNDELL, KRISTINA BJÖRNSSON, BJÖRN THRANDUR 1988-11-01 00:00:00.0 text/html http://jeb.biologists.org/cgi/content/short/140/1/171 en eng Company of Biologists http://jeb.biologists.org/cgi/content/short/140/1/171 Copyright (C) 1988, Company of Biologists Journal Articles TEXT 1988 fthighwire 2013-04-02T07:08:07Z An in vitro technique for perfusion of the intestinal vasculature and lumen was developed and used to measure calcium (Ca2+) fluxes across the intestinal mucosa of the marine teleost, the Atlantic cod ( Gadus morhua ). Saturable and nonsaturable components of the calcium influx and efflux were determined. The calcium influx had one passive component and one saturable component, following Michaelis-Menten kinetics with K m = 8.41mmoll-1 and V max = 0.604µmol Ca2+ kg-1 h-1. At physiological Ca2+ concentrations in the vascular ([Ca2+] = l.9mmoll-1) and luminal ([Ca2+] =14.9mmoll-1) perfusion fluids, the saturable component amounted to 60% of the Ca2+ influx. The high-affinity Ca2+-ATPase inhibitor chlorpromazine (CP, 10-4moll-1) antagonized 45% of the Ca2+ influx. The Ca2+ efflux across the intestinal mucosa of the cod was a saturable process, following Michaelis-Menten kinetics with K m =6.15mmoll-1 and V max =3.79µmol Ca2+ kg-1h-1, but insensitive to CP (l0-5moll-1) . The Ca2+ efflux was l.22µumol Ca2+ kg-1 h-1, representing about 20% of the total calcium excretion and about 50% of the extrarenal excretion of the Atlantic cod in vivo . Text atlantic cod Gadus morhua HighWire Press (Stanford University) |
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Journal Articles SUNDELL, KRISTINA BJÖRNSSON, BJÖRN THRANDUR Kinetics of Calcium Fluxes Across the Intestinal Mucosa of the Marine Teleost, Gadus Morhua, Measured Using an In Vitro Perfusion Method |
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Journal Articles |
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An in vitro technique for perfusion of the intestinal vasculature and lumen was developed and used to measure calcium (Ca2+) fluxes across the intestinal mucosa of the marine teleost, the Atlantic cod ( Gadus morhua ). Saturable and nonsaturable components of the calcium influx and efflux were determined. The calcium influx had one passive component and one saturable component, following Michaelis-Menten kinetics with K m = 8.41mmoll-1 and V max = 0.604µmol Ca2+ kg-1 h-1. At physiological Ca2+ concentrations in the vascular ([Ca2+] = l.9mmoll-1) and luminal ([Ca2+] =14.9mmoll-1) perfusion fluids, the saturable component amounted to 60% of the Ca2+ influx. The high-affinity Ca2+-ATPase inhibitor chlorpromazine (CP, 10-4moll-1) antagonized 45% of the Ca2+ influx. The Ca2+ efflux across the intestinal mucosa of the cod was a saturable process, following Michaelis-Menten kinetics with K m =6.15mmoll-1 and V max =3.79µmol Ca2+ kg-1h-1, but insensitive to CP (l0-5moll-1) . The Ca2+ efflux was l.22µumol Ca2+ kg-1 h-1, representing about 20% of the total calcium excretion and about 50% of the extrarenal excretion of the Atlantic cod in vivo . |
format |
Text |
author |
SUNDELL, KRISTINA BJÖRNSSON, BJÖRN THRANDUR |
author_facet |
SUNDELL, KRISTINA BJÖRNSSON, BJÖRN THRANDUR |
author_sort |
SUNDELL, KRISTINA |
title |
Kinetics of Calcium Fluxes Across the Intestinal Mucosa of the Marine Teleost, Gadus Morhua, Measured Using an In Vitro Perfusion Method |
title_short |
Kinetics of Calcium Fluxes Across the Intestinal Mucosa of the Marine Teleost, Gadus Morhua, Measured Using an In Vitro Perfusion Method |
title_full |
Kinetics of Calcium Fluxes Across the Intestinal Mucosa of the Marine Teleost, Gadus Morhua, Measured Using an In Vitro Perfusion Method |
title_fullStr |
Kinetics of Calcium Fluxes Across the Intestinal Mucosa of the Marine Teleost, Gadus Morhua, Measured Using an In Vitro Perfusion Method |
title_full_unstemmed |
Kinetics of Calcium Fluxes Across the Intestinal Mucosa of the Marine Teleost, Gadus Morhua, Measured Using an In Vitro Perfusion Method |
title_sort |
kinetics of calcium fluxes across the intestinal mucosa of the marine teleost, gadus morhua, measured using an in vitro perfusion method |
publisher |
Company of Biologists |
publishDate |
1988 |
url |
http://jeb.biologists.org/cgi/content/short/140/1/171 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
http://jeb.biologists.org/cgi/content/short/140/1/171 |
op_rights |
Copyright (C) 1988, Company of Biologists |
_version_ |
1766357641506848768 |