Isolation and Partial Characterization of Growth Hormone from Fin Whale Pituitary Glands
A highly purified somatotropin (growth hormone) has been isolated from fin whale ( Balaenoptera physalus ) pituitary glands in a yield of 1,014 mg/kg of wet weight tissue. The preparation was shown to be homogenous by isoelectric focusing on polyacrylamide with growthpromoting potency comparable to...
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1980
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fthighwire:oai:open-archive.highwire.org:jbiochem:88/5/1407 2023-05-15T15:36:36+02:00 Isolation and Partial Characterization of Growth Hormone from Fin Whale Pituitary Glands TSUBOKAWA, Makoto KAWAUCHI, Hiroshi LI, Choh Hao 1980-10-01 00:00:00.0 text/html http://jb.oxfordjournals.org/cgi/content/short/88/5/1407 en eng Oxford University Press http://jb.oxfordjournals.org/cgi/content/short/88/5/1407 Copyright (C) 1980, Japanese Biochemical Society Articles TEXT 1980 fthighwire 2016-11-16T18:03:19Z A highly purified somatotropin (growth hormone) has been isolated from fin whale ( Balaenoptera physalus ) pituitary glands in a yield of 1,014 mg/kg of wet weight tissue. The preparation was shown to be homogenous by isoelectric focusing on polyacrylamide with growthpromoting potency comparable to that of ovine growth hormone by the rat tibia test. The molecular weight was found to be 20,000 daltons by SDS gel electrophoresis and 40,000 daltons by exclusion chromatography on Sephadex G-100 at pH 8.2. The amino acid composition and α-helix content of fin whale somatotropin were similar to those of human, bovine, and ovine hormones. The circular dichroism in the side chain region, however, differed from those of the mammalian hormones. The NH 2 -terminal amino acid sequence was nearly identical with those of the bovine and ovine hormones as determined by the fluorescein isothiocyanate method. Text Balaenoptera physalus Fin whale HighWire Press (Stanford University) |
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Articles TSUBOKAWA, Makoto KAWAUCHI, Hiroshi LI, Choh Hao Isolation and Partial Characterization of Growth Hormone from Fin Whale Pituitary Glands |
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description |
A highly purified somatotropin (growth hormone) has been isolated from fin whale ( Balaenoptera physalus ) pituitary glands in a yield of 1,014 mg/kg of wet weight tissue. The preparation was shown to be homogenous by isoelectric focusing on polyacrylamide with growthpromoting potency comparable to that of ovine growth hormone by the rat tibia test. The molecular weight was found to be 20,000 daltons by SDS gel electrophoresis and 40,000 daltons by exclusion chromatography on Sephadex G-100 at pH 8.2. The amino acid composition and α-helix content of fin whale somatotropin were similar to those of human, bovine, and ovine hormones. The circular dichroism in the side chain region, however, differed from those of the mammalian hormones. The NH 2 -terminal amino acid sequence was nearly identical with those of the bovine and ovine hormones as determined by the fluorescein isothiocyanate method. |
format |
Text |
author |
TSUBOKAWA, Makoto KAWAUCHI, Hiroshi LI, Choh Hao |
author_facet |
TSUBOKAWA, Makoto KAWAUCHI, Hiroshi LI, Choh Hao |
author_sort |
TSUBOKAWA, Makoto |
title |
Isolation and Partial Characterization of Growth Hormone from Fin Whale Pituitary Glands |
title_short |
Isolation and Partial Characterization of Growth Hormone from Fin Whale Pituitary Glands |
title_full |
Isolation and Partial Characterization of Growth Hormone from Fin Whale Pituitary Glands |
title_fullStr |
Isolation and Partial Characterization of Growth Hormone from Fin Whale Pituitary Glands |
title_full_unstemmed |
Isolation and Partial Characterization of Growth Hormone from Fin Whale Pituitary Glands |
title_sort |
isolation and partial characterization of growth hormone from fin whale pituitary glands |
publisher |
Oxford University Press |
publishDate |
1980 |
url |
http://jb.oxfordjournals.org/cgi/content/short/88/5/1407 |
genre |
Balaenoptera physalus Fin whale |
genre_facet |
Balaenoptera physalus Fin whale |
op_relation |
http://jb.oxfordjournals.org/cgi/content/short/88/5/1407 |
op_rights |
Copyright (C) 1980, Japanese Biochemical Society |
_version_ |
1766366974787452928 |