Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases

The separation and purification of three kinds of proteolytic enzymes from fin whale pancreas are described. An acid extract of fin whale pancreas was fractionated with (NH 4 ) 2 SO 4 and treated with trypsin [EC 3.4.4.4]. An anionic chymotrypsin [EC 3.4.4.5] and a cationic proteolytic enzyme fracti...

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Main Authors: KOIDE, ATSUSHI, MATSUOKA, YOSHITAKA
Format: Text
Language:English
Published: Oxford University Press 1969
Subjects:
Articles
Fin whale
Online Access:http://jb.oxfordjournals.org/cgi/content/short/66/4/541
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spelling fthighwire:oai:open-archive.highwire.org:jbiochem:66/4/541 2023-05-15T16:13:16+02:00 Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases KOIDE, ATSUSHI MATSUOKA, YOSHITAKA 1969-10-01 00:00:00.0 text/html http://jb.oxfordjournals.org/cgi/content/short/66/4/541 en eng Oxford University Press http://jb.oxfordjournals.org/cgi/content/short/66/4/541 Copyright (C) 1969, Japanese Biochemical Society Articles TEXT 1969 fthighwire 2013-05-28T03:51:57Z The separation and purification of three kinds of proteolytic enzymes from fin whale pancreas are described. An acid extract of fin whale pancreas was fractionated with (NH 4 ) 2 SO 4 and treated with trypsin [EC 3.4.4.4]. An anionic chymotrypsin [EC 3.4.4.5] and a cationic proteolytic enzyme fraction in this crude proteolytic enzyme preparation were roughly separated by CM-Sephadex column chromatography. Cationic chymotrypsin and trypsin were obtained from the cationic proteolytic enzyme fraction by CM-cellulose column chromatography. These proteolytic enzymes were purified further by rechromatography on either CM-Sephadex or CM-cellulose. Text Fin whale HighWire Press (Stanford University)
institution Open Polar
collection HighWire Press (Stanford University)
op_collection_id fthighwire
language English
topic Articles
spellingShingle Articles
KOIDE, ATSUSHI
MATSUOKA, YOSHITAKA
Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases
topic_facet Articles
description The separation and purification of three kinds of proteolytic enzymes from fin whale pancreas are described. An acid extract of fin whale pancreas was fractionated with (NH 4 ) 2 SO 4 and treated with trypsin [EC 3.4.4.4]. An anionic chymotrypsin [EC 3.4.4.5] and a cationic proteolytic enzyme fraction in this crude proteolytic enzyme preparation were roughly separated by CM-Sephadex column chromatography. Cationic chymotrypsin and trypsin were obtained from the cationic proteolytic enzyme fraction by CM-cellulose column chromatography. These proteolytic enzymes were purified further by rechromatography on either CM-Sephadex or CM-cellulose.
format Text
author KOIDE, ATSUSHI
MATSUOKA, YOSHITAKA
author_facet KOIDE, ATSUSHI
MATSUOKA, YOSHITAKA
author_sort KOIDE, ATSUSHI
title Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases
title_short Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases
title_full Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases
title_fullStr Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases
title_full_unstemmed Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases
title_sort studies on fin whale pancreatic proteases: ii. purification of the pancreatic proteases
publisher Oxford University Press
publishDate 1969
url http://jb.oxfordjournals.org/cgi/content/short/66/4/541
genre Fin whale
genre_facet Fin whale
op_relation http://jb.oxfordjournals.org/cgi/content/short/66/4/541
op_rights Copyright (C) 1969, Japanese Biochemical Society
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