Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases
The separation and purification of three kinds of proteolytic enzymes from fin whale pancreas are described. An acid extract of fin whale pancreas was fractionated with (NH 4 ) 2 SO 4 and treated with trypsin [EC 3.4.4.4]. An anionic chymotrypsin [EC 3.4.4.5] and a cationic proteolytic enzyme fracti...
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1969
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fthighwire:oai:open-archive.highwire.org:jbiochem:66/4/541 2023-05-15T16:13:16+02:00 Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases KOIDE, ATSUSHI MATSUOKA, YOSHITAKA 1969-10-01 00:00:00.0 text/html http://jb.oxfordjournals.org/cgi/content/short/66/4/541 en eng Oxford University Press http://jb.oxfordjournals.org/cgi/content/short/66/4/541 Copyright (C) 1969, Japanese Biochemical Society Articles TEXT 1969 fthighwire 2013-05-28T03:51:57Z The separation and purification of three kinds of proteolytic enzymes from fin whale pancreas are described. An acid extract of fin whale pancreas was fractionated with (NH 4 ) 2 SO 4 and treated with trypsin [EC 3.4.4.4]. An anionic chymotrypsin [EC 3.4.4.5] and a cationic proteolytic enzyme fraction in this crude proteolytic enzyme preparation were roughly separated by CM-Sephadex column chromatography. Cationic chymotrypsin and trypsin were obtained from the cationic proteolytic enzyme fraction by CM-cellulose column chromatography. These proteolytic enzymes were purified further by rechromatography on either CM-Sephadex or CM-cellulose. Text Fin whale HighWire Press (Stanford University) |
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Articles |
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Articles KOIDE, ATSUSHI MATSUOKA, YOSHITAKA Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases |
topic_facet |
Articles |
description |
The separation and purification of three kinds of proteolytic enzymes from fin whale pancreas are described. An acid extract of fin whale pancreas was fractionated with (NH 4 ) 2 SO 4 and treated with trypsin [EC 3.4.4.4]. An anionic chymotrypsin [EC 3.4.4.5] and a cationic proteolytic enzyme fraction in this crude proteolytic enzyme preparation were roughly separated by CM-Sephadex column chromatography. Cationic chymotrypsin and trypsin were obtained from the cationic proteolytic enzyme fraction by CM-cellulose column chromatography. These proteolytic enzymes were purified further by rechromatography on either CM-Sephadex or CM-cellulose. |
format |
Text |
author |
KOIDE, ATSUSHI MATSUOKA, YOSHITAKA |
author_facet |
KOIDE, ATSUSHI MATSUOKA, YOSHITAKA |
author_sort |
KOIDE, ATSUSHI |
title |
Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases |
title_short |
Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases |
title_full |
Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases |
title_fullStr |
Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases |
title_full_unstemmed |
Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases |
title_sort |
studies on fin whale pancreatic proteases: ii. purification of the pancreatic proteases |
publisher |
Oxford University Press |
publishDate |
1969 |
url |
http://jb.oxfordjournals.org/cgi/content/short/66/4/541 |
genre |
Fin whale |
genre_facet |
Fin whale |
op_relation |
http://jb.oxfordjournals.org/cgi/content/short/66/4/541 |
op_rights |
Copyright (C) 1969, Japanese Biochemical Society |
_version_ |
1765998920967651328 |