Studies on Fin Whale Pancreatic Proteases: II. Purification of the Pancreatic Proteases
The separation and purification of three kinds of proteolytic enzymes from fin whale pancreas are described. An acid extract of fin whale pancreas was fractionated with (NH 4 ) 2 SO 4 and treated with trypsin [EC 3.4.4.4]. An anionic chymotrypsin [EC 3.4.4.5] and a cationic proteolytic enzyme fracti...
| Main Authors: | , |
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| Format: | Text |
| Language: | English |
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Oxford University Press
1969
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| Subjects: | |
| Online Access: | http://jb.oxfordjournals.org/cgi/content/short/66/4/541 |
| Summary: | The separation and purification of three kinds of proteolytic enzymes from fin whale pancreas are described. An acid extract of fin whale pancreas was fractionated with (NH 4 ) 2 SO 4 and treated with trypsin [EC 3.4.4.4]. An anionic chymotrypsin [EC 3.4.4.5] and a cationic proteolytic enzyme fraction in this crude proteolytic enzyme preparation were roughly separated by CM-Sephadex column chromatography. Cationic chymotrypsin and trypsin were obtained from the cationic proteolytic enzyme fraction by CM-cellulose column chromatography. These proteolytic enzymes were purified further by rechromatography on either CM-Sephadex or CM-cellulose. |
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