Crystal Structures of Modified Myoglobins. I. Heme Orientation and Structural Changes around Heme in Myoglobins Reconstituted with Isopemptoheme, Pemptoheme, 2-Ethyldeuteroheme, and 4-Ethyldeuteroheme
The crystal structures of sperm whale metmyoglobins reconstituted with four modified hemes, isopemptoheme, pemptoheme, 2-ethyldeuteroheme, and 4-ethyldeutero-heme, have been determined and refined at 2.2 Å resolution to R =0.217, 0.218, 0.213, and 0.222, respectively. All the crystals of these myogl...
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1986
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fthighwire:oai:open-archive.highwire.org:jbiochem:100/2/269 2023-05-15T18:26:44+02:00 Crystal Structures of Modified Myoglobins. I. Heme Orientation and Structural Changes around Heme in Myoglobins Reconstituted with Isopemptoheme, Pemptoheme, 2-Ethyldeuteroheme, and 4-Ethyldeuteroheme MIKI, Kunio Yasuaki, YUKAWA, Motomu OWATARI, Akira HATO, Yukinori HARADA, Shigeharu KAI, Yasushi KASAI, Nobutami HATA, Yasuo TANAKA, Nobuo KAKUDO, Masao KATSUBE, Yukiteru KAWABE, Kuniyasu YOSHTOA, Zen-ichi OGOSHI, Hisanobu 1986-01-01 00:00:00.0 text/html http://jb.oxfordjournals.org/cgi/content/short/100/2/269 en eng Oxford University Press http://jb.oxfordjournals.org/cgi/content/short/100/2/269 Copyright (C) 1986, Japanese Biochemical Society REGULAR PAPERS TEXT 1986 fthighwire 2013-05-27T14:04:22Z The crystal structures of sperm whale metmyoglobins reconstituted with four modified hemes, isopemptoheme, pemptoheme, 2-ethyldeuteroheme, and 4-ethyldeutero-heme, have been determined and refined at 2.2 Å resolution to R =0.217, 0.218, 0.213, and 0.222, respectively. All the crystals of these myoglobins are isomorphous with that of native metmyoglobin. The structural changes of the modified myoglobin from the native myoglobin were examined on difference Fourier maps; the orientation of 4-ethyldeuteroheme in the heme pocket is such that the heme is rotated by 180° about an axis through the α-γ-meso carbons, whereas the orientations of the other three hemes are the same as that of the protoheme in the native myoglobin. The changes of the structures around the heme become greater in the order of isopemptoheme, 2-ethyldeuteroheme < pemptoheme < 4-ethyldeuteroheme. The magnitudes of the changes seem to be related to the oxygen affinities of these four reconstituted myoglobins. Text Sperm whale HighWire Press (Stanford University) |
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HighWire Press (Stanford University) |
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English |
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REGULAR PAPERS |
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REGULAR PAPERS MIKI, Kunio Yasuaki, YUKAWA, Motomu OWATARI, Akira HATO, Yukinori HARADA, Shigeharu KAI, Yasushi KASAI, Nobutami HATA, Yasuo TANAKA, Nobuo KAKUDO, Masao KATSUBE, Yukiteru KAWABE, Kuniyasu YOSHTOA, Zen-ichi OGOSHI, Hisanobu Crystal Structures of Modified Myoglobins. I. Heme Orientation and Structural Changes around Heme in Myoglobins Reconstituted with Isopemptoheme, Pemptoheme, 2-Ethyldeuteroheme, and 4-Ethyldeuteroheme |
topic_facet |
REGULAR PAPERS |
description |
The crystal structures of sperm whale metmyoglobins reconstituted with four modified hemes, isopemptoheme, pemptoheme, 2-ethyldeuteroheme, and 4-ethyldeutero-heme, have been determined and refined at 2.2 Å resolution to R =0.217, 0.218, 0.213, and 0.222, respectively. All the crystals of these myoglobins are isomorphous with that of native metmyoglobin. The structural changes of the modified myoglobin from the native myoglobin were examined on difference Fourier maps; the orientation of 4-ethyldeuteroheme in the heme pocket is such that the heme is rotated by 180° about an axis through the α-γ-meso carbons, whereas the orientations of the other three hemes are the same as that of the protoheme in the native myoglobin. The changes of the structures around the heme become greater in the order of isopemptoheme, 2-ethyldeuteroheme < pemptoheme < 4-ethyldeuteroheme. The magnitudes of the changes seem to be related to the oxygen affinities of these four reconstituted myoglobins. |
format |
Text |
author |
MIKI, Kunio Yasuaki, YUKAWA, Motomu OWATARI, Akira HATO, Yukinori HARADA, Shigeharu KAI, Yasushi KASAI, Nobutami HATA, Yasuo TANAKA, Nobuo KAKUDO, Masao KATSUBE, Yukiteru KAWABE, Kuniyasu YOSHTOA, Zen-ichi OGOSHI, Hisanobu |
author_facet |
MIKI, Kunio Yasuaki, YUKAWA, Motomu OWATARI, Akira HATO, Yukinori HARADA, Shigeharu KAI, Yasushi KASAI, Nobutami HATA, Yasuo TANAKA, Nobuo KAKUDO, Masao KATSUBE, Yukiteru KAWABE, Kuniyasu YOSHTOA, Zen-ichi OGOSHI, Hisanobu |
author_sort |
MIKI, Kunio |
title |
Crystal Structures of Modified Myoglobins. I. Heme Orientation and Structural Changes around Heme in Myoglobins Reconstituted with Isopemptoheme, Pemptoheme, 2-Ethyldeuteroheme, and 4-Ethyldeuteroheme |
title_short |
Crystal Structures of Modified Myoglobins. I. Heme Orientation and Structural Changes around Heme in Myoglobins Reconstituted with Isopemptoheme, Pemptoheme, 2-Ethyldeuteroheme, and 4-Ethyldeuteroheme |
title_full |
Crystal Structures of Modified Myoglobins. I. Heme Orientation and Structural Changes around Heme in Myoglobins Reconstituted with Isopemptoheme, Pemptoheme, 2-Ethyldeuteroheme, and 4-Ethyldeuteroheme |
title_fullStr |
Crystal Structures of Modified Myoglobins. I. Heme Orientation and Structural Changes around Heme in Myoglobins Reconstituted with Isopemptoheme, Pemptoheme, 2-Ethyldeuteroheme, and 4-Ethyldeuteroheme |
title_full_unstemmed |
Crystal Structures of Modified Myoglobins. I. Heme Orientation and Structural Changes around Heme in Myoglobins Reconstituted with Isopemptoheme, Pemptoheme, 2-Ethyldeuteroheme, and 4-Ethyldeuteroheme |
title_sort |
crystal structures of modified myoglobins. i. heme orientation and structural changes around heme in myoglobins reconstituted with isopemptoheme, pemptoheme, 2-ethyldeuteroheme, and 4-ethyldeuteroheme |
publisher |
Oxford University Press |
publishDate |
1986 |
url |
http://jb.oxfordjournals.org/cgi/content/short/100/2/269 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_relation |
http://jb.oxfordjournals.org/cgi/content/short/100/2/269 |
op_rights |
Copyright (C) 1986, Japanese Biochemical Society |
_version_ |
1766208708333797376 |