Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli
Objectives : To determine the level of antimicrobial resistance and the occurrence of class 1, 2 and 3 integrons in faecal Escherichia coli from several animal populations variously exposed to human contact. Methods : A collection of 341 faecal E. coli isolates was constituted from several animal po...
Published in: | Journal of Antimicrobial Chemotherapy |
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fthighwire:oai:open-archive.highwire.org:jac:dkl122v1 2023-05-15T13:55:40+02:00 Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli Skurnik, David Ruimy, Raymond Andremont, Antoine Amorin, Christine Rouquet, Pierre Picard, Bertrand Denamur, Erick 2006-03-31 09:07:28.0 text/html http://jac.oxfordjournals.org/cgi/content/short/dkl122v1 https://doi.org/10.1093/jac/dkl122 en eng Oxford University Press http://jac.oxfordjournals.org/cgi/content/short/dkl122v1 http://dx.doi.org/10.1093/jac/dkl122 Copyright (C) 2006, The British Society for Antimicrobial Chemotherapy Brief report TEXT 2006 fthighwire https://doi.org/10.1093/jac/dkl122 2016-11-16T17:41:49Z Objectives : To determine the level of antimicrobial resistance and the occurrence of class 1, 2 and 3 integrons in faecal Escherichia coli from several animal populations variously exposed to human contact. Methods : A collection of 341 faecal E. coli isolates was constituted from several animal populations subject to various degrees of exposure to humans: 18 animals never exposed to humans (living in the Antarctic or Gabon), 71 wild animals living in a low human density area (mountainous region of the Pyrenees, France), 61 wild animals living in a higher human density area (Fontainebleau forest near Paris, France), and 128 extensively reared farm animals and 42 pet dogs, both living in the Pyrenees. Resistance to antimicrobial agents was determined by the method of disc diffusion and quantified using the resistance score of BE Murray, JJ Mathewson, HL DuPont, CD Ericsson and RR Reves (Antimicrobial Agents and Chemotherapy 1990; 34: 515-18). Integrons were characterized by triplex real-time PCR and sequencing. The absence of epidemiologic clones was confirmed by PCR-based methods. Results : A gradient of resistance ranging from absence to high prevalence (resistance score of 18.7%) and a gradual increase in the prevalence of class 1 integrons (from 0% to 16%), both correlated with the increase in exposure to humans, were observed. In wild animals with little contact with humans, resistance, when present, was not mediated by integrons. Conclusions : Our findings firmly establish that the current prevalence of antimicrobial resistance found in animal faecal bacteria, as well as the prevalence of integrons, is clearly anthropogenic. The presence of integrons may constitute an adaptive process to environments whose antimicrobial pressure exceeds a certain threshold. Text Antarc* Antarctic HighWire Press (Stanford University) Antarctic The Antarctic Journal of Antimicrobial Chemotherapy 57 6 1215 1219 |
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Brief report Skurnik, David Ruimy, Raymond Andremont, Antoine Amorin, Christine Rouquet, Pierre Picard, Bertrand Denamur, Erick Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli |
topic_facet |
Brief report |
description |
Objectives : To determine the level of antimicrobial resistance and the occurrence of class 1, 2 and 3 integrons in faecal Escherichia coli from several animal populations variously exposed to human contact. Methods : A collection of 341 faecal E. coli isolates was constituted from several animal populations subject to various degrees of exposure to humans: 18 animals never exposed to humans (living in the Antarctic or Gabon), 71 wild animals living in a low human density area (mountainous region of the Pyrenees, France), 61 wild animals living in a higher human density area (Fontainebleau forest near Paris, France), and 128 extensively reared farm animals and 42 pet dogs, both living in the Pyrenees. Resistance to antimicrobial agents was determined by the method of disc diffusion and quantified using the resistance score of BE Murray, JJ Mathewson, HL DuPont, CD Ericsson and RR Reves (Antimicrobial Agents and Chemotherapy 1990; 34: 515-18). Integrons were characterized by triplex real-time PCR and sequencing. The absence of epidemiologic clones was confirmed by PCR-based methods. Results : A gradient of resistance ranging from absence to high prevalence (resistance score of 18.7%) and a gradual increase in the prevalence of class 1 integrons (from 0% to 16%), both correlated with the increase in exposure to humans, were observed. In wild animals with little contact with humans, resistance, when present, was not mediated by integrons. Conclusions : Our findings firmly establish that the current prevalence of antimicrobial resistance found in animal faecal bacteria, as well as the prevalence of integrons, is clearly anthropogenic. The presence of integrons may constitute an adaptive process to environments whose antimicrobial pressure exceeds a certain threshold. |
format |
Text |
author |
Skurnik, David Ruimy, Raymond Andremont, Antoine Amorin, Christine Rouquet, Pierre Picard, Bertrand Denamur, Erick |
author_facet |
Skurnik, David Ruimy, Raymond Andremont, Antoine Amorin, Christine Rouquet, Pierre Picard, Bertrand Denamur, Erick |
author_sort |
Skurnik, David |
title |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli |
title_short |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli |
title_full |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli |
title_fullStr |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli |
title_full_unstemmed |
Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli |
title_sort |
effect of human vicinity on antimicrobial resistance and integrons in animal faecal escherichia coli |
publisher |
Oxford University Press |
publishDate |
2006 |
url |
http://jac.oxfordjournals.org/cgi/content/short/dkl122v1 https://doi.org/10.1093/jac/dkl122 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://jac.oxfordjournals.org/cgi/content/short/dkl122v1 http://dx.doi.org/10.1093/jac/dkl122 |
op_rights |
Copyright (C) 2006, The British Society for Antimicrobial Chemotherapy |
op_doi |
https://doi.org/10.1093/jac/dkl122 |
container_title |
Journal of Antimicrobial Chemotherapy |
container_volume |
57 |
container_issue |
6 |
container_start_page |
1215 |
op_container_end_page |
1219 |
_version_ |
1766262449768497152 |