Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife
Objectives A recently identified mecA homologue, mecC , in methicillin-resistant Staphylococcus aureus (MRSA) has been isolated from humans and different animal hosts. The aim of this study was to determine antimicrobial resistance and provide molecular characterization of MRSA and methicillin-resis...
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fthighwire:oai:open-archive.highwire.org:jac:68/10/2222 2023-05-15T18:50:18+02:00 Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife Loncaric, Igor Kübber-Heiss, Anna Posautz, Annika Stalder, Gabrielle L. Hoffmann, Daniel Rosengarten, Renate Walzer, Chris 2013-10-01 00:00:00.0 text/html http://jac.oxfordjournals.org/cgi/content/short/68/10/2222 https://doi.org/10.1093/jac/dkt186 en eng Oxford University Press http://jac.oxfordjournals.org/cgi/content/short/68/10/2222 http://dx.doi.org/10.1093/jac/dkt186 Copyright (C) 2013, The British Society for Antimicrobial Chemotherapy Original research TEXT 2013 fthighwire https://doi.org/10.1093/jac/dkt186 2015-02-28T23:24:25Z Objectives A recently identified mecA homologue, mecC , in methicillin-resistant Staphylococcus aureus (MRSA) has been isolated from humans and different animal hosts. The aim of this study was to determine antimicrobial resistance and provide molecular characterization of MRSA and methicillin-resistant non- Staphylococcus aureus staphylococci (MRnSA) isolated from wildlife that carried the gene mecC . Methods Five S. aureus and one coagulase-negative Staphylococcus isolate displaying phenotypic oxacillin resistance, but not recognized with conventional PCR for mecA , were further characterized by a polyphasic approach. The presence of mecC in all isolates was determined using specific PCR. PCR targeting Panton–Valentine leucocidin (PVL) genes of MRSA was performed. MRSA isolates were genotyped by spa typing and multilocus sequence typing. All isolates were genotyped by staphylococcal cassette chromosome mec (SCC mec ) typing. 16S rDNA sequence analysis for MRnSA identification was performed. Antimicrobial susceptibility testing was performed for all isolates. Results All five MRSA isolates contained the mecC gene, were PVL negative, carried SCC mec type XI and belonged to ST130 (where ST stands for sequence type), with spa types t843, t10513 or t3256, or to ST2620, with spa type t4335. The MRnSA isolate, most closely related to Staphylococcus stepanovicii , carried mecA and bla Z genes related to SCC mec XI. MRSA isolates exhibited resistance to the β-lactams only. Conclusions The MRSA isolates described in this study represent the first detection of mecC -positive MRSA in a European otter ( Lutra lutra ) and a European brown hare ( Lepus europaeus ). The MRnSA isolate represents the first isolation of MRnSA from a Eurasian lynx ( Lynx lynx ). Text Lutra lutra Lynx Lynx lynx lynx HighWire Press (Stanford University) Valentine ENVELOPE(-54.550,-54.550,-61.083,-61.083) Journal of Antimicrobial Chemotherapy |
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HighWire Press (Stanford University) |
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Original research Loncaric, Igor Kübber-Heiss, Anna Posautz, Annika Stalder, Gabrielle L. Hoffmann, Daniel Rosengarten, Renate Walzer, Chris Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife |
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Original research |
description |
Objectives A recently identified mecA homologue, mecC , in methicillin-resistant Staphylococcus aureus (MRSA) has been isolated from humans and different animal hosts. The aim of this study was to determine antimicrobial resistance and provide molecular characterization of MRSA and methicillin-resistant non- Staphylococcus aureus staphylococci (MRnSA) isolated from wildlife that carried the gene mecC . Methods Five S. aureus and one coagulase-negative Staphylococcus isolate displaying phenotypic oxacillin resistance, but not recognized with conventional PCR for mecA , were further characterized by a polyphasic approach. The presence of mecC in all isolates was determined using specific PCR. PCR targeting Panton–Valentine leucocidin (PVL) genes of MRSA was performed. MRSA isolates were genotyped by spa typing and multilocus sequence typing. All isolates were genotyped by staphylococcal cassette chromosome mec (SCC mec ) typing. 16S rDNA sequence analysis for MRnSA identification was performed. Antimicrobial susceptibility testing was performed for all isolates. Results All five MRSA isolates contained the mecC gene, were PVL negative, carried SCC mec type XI and belonged to ST130 (where ST stands for sequence type), with spa types t843, t10513 or t3256, or to ST2620, with spa type t4335. The MRnSA isolate, most closely related to Staphylococcus stepanovicii , carried mecA and bla Z genes related to SCC mec XI. MRSA isolates exhibited resistance to the β-lactams only. Conclusions The MRSA isolates described in this study represent the first detection of mecC -positive MRSA in a European otter ( Lutra lutra ) and a European brown hare ( Lepus europaeus ). The MRnSA isolate represents the first isolation of MRnSA from a Eurasian lynx ( Lynx lynx ). |
format |
Text |
author |
Loncaric, Igor Kübber-Heiss, Anna Posautz, Annika Stalder, Gabrielle L. Hoffmann, Daniel Rosengarten, Renate Walzer, Chris |
author_facet |
Loncaric, Igor Kübber-Heiss, Anna Posautz, Annika Stalder, Gabrielle L. Hoffmann, Daniel Rosengarten, Renate Walzer, Chris |
author_sort |
Loncaric, Igor |
title |
Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife |
title_short |
Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife |
title_full |
Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife |
title_fullStr |
Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife |
title_full_unstemmed |
Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife |
title_sort |
characterization of methicillin-resistant staphylococcus spp. carrying the mecc gene, isolated from wildlife |
publisher |
Oxford University Press |
publishDate |
2013 |
url |
http://jac.oxfordjournals.org/cgi/content/short/68/10/2222 https://doi.org/10.1093/jac/dkt186 |
long_lat |
ENVELOPE(-54.550,-54.550,-61.083,-61.083) |
geographic |
Valentine |
geographic_facet |
Valentine |
genre |
Lutra lutra Lynx Lynx lynx lynx |
genre_facet |
Lutra lutra Lynx Lynx lynx lynx |
op_relation |
http://jac.oxfordjournals.org/cgi/content/short/68/10/2222 http://dx.doi.org/10.1093/jac/dkt186 |
op_rights |
Copyright (C) 2013, The British Society for Antimicrobial Chemotherapy |
op_doi |
https://doi.org/10.1093/jac/dkt186 |
container_title |
Journal of Antimicrobial Chemotherapy |
_version_ |
1766244007999963136 |