Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes
Otolith marking with enriched stable isotopes via immersion is a recent method of batch marking larval fish for a range of research and industrial applications. However, current immersion times and isotope concentrations required to successfully mark an otolith limit the utility of this technique. O...
Published in: | ICES Journal of Marine Science |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Oxford University Press
2014
|
Subjects: | |
Online Access: | http://icesjms.oxfordjournals.org/cgi/content/short/71/9/2530 https://doi.org/10.1093/icesjms/fsu091 |
id |
fthighwire:oai:open-archive.highwire.org:icesjms:71/9/2530 |
---|---|
record_format |
openpolar |
spelling |
fthighwire:oai:open-archive.highwire.org:icesjms:71/9/2530 2023-05-15T15:32:27+02:00 Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes de Braux, Emmanuel Warren-Myers, Fletcher Dempster, Tim Fjelldal, Per Gunnar Hansen, Tom Swearer, Stephen E. 2014-11-01 00:00:00.0 text/html http://icesjms.oxfordjournals.org/cgi/content/short/71/9/2530 https://doi.org/10.1093/icesjms/fsu091 en eng Oxford University Press http://icesjms.oxfordjournals.org/cgi/content/short/71/9/2530 http://dx.doi.org/10.1093/icesjms/fsu091 Copyright (C) 2014, International Council for the Exploration of the Sea/Conseil International pour l'Exploration de la Mer Original Articles TEXT 2014 fthighwire https://doi.org/10.1093/icesjms/fsu091 2015-02-28T22:22:56Z Otolith marking with enriched stable isotopes via immersion is a recent method of batch marking larval fish for a range of research and industrial applications. However, current immersion times and isotope concentrations required to successfully mark an otolith limit the utility of this technique. Osmotic induction improves incorporation and reduces immersion time for some chemical markers, but its effects on isotope incorporation into otoliths are unknown. Here, we tested the effects of osmotic induction over a range of different isotope concentrations and immersion times on relative mark success and strength for 26Mg:24Mg, 86Sr:88Sr and 137Ba:138Ba on Atlantic salmon ( Salmo salar ) larvae. 71% and 100% mark success were achieved after 1 h of immersion for 86Sr (75 µg L−1) and 137Ba (30 µg L−1) isotopes, respectively. Compared with conventional immersion, osmotic induction improved overall mark strength for 86Sr and 137Ba isotopes by 26–116%, although this effect was only observed after 12 h of immersion and predominately for 86Sr. The results demonstrate that osmotic induction reduces immersion times and the concentrations of isotope required to achieve successful marks. Osmotically induced isotope labels via larval immersion may prove a rapid and cost-effective way of batch marking fish larvae across a range of potential applications. Text Atlantic salmon Salmo salar HighWire Press (Stanford University) ICES Journal of Marine Science 71 9 2530 2538 |
institution |
Open Polar |
collection |
HighWire Press (Stanford University) |
op_collection_id |
fthighwire |
language |
English |
topic |
Original Articles |
spellingShingle |
Original Articles de Braux, Emmanuel Warren-Myers, Fletcher Dempster, Tim Fjelldal, Per Gunnar Hansen, Tom Swearer, Stephen E. Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
topic_facet |
Original Articles |
description |
Otolith marking with enriched stable isotopes via immersion is a recent method of batch marking larval fish for a range of research and industrial applications. However, current immersion times and isotope concentrations required to successfully mark an otolith limit the utility of this technique. Osmotic induction improves incorporation and reduces immersion time for some chemical markers, but its effects on isotope incorporation into otoliths are unknown. Here, we tested the effects of osmotic induction over a range of different isotope concentrations and immersion times on relative mark success and strength for 26Mg:24Mg, 86Sr:88Sr and 137Ba:138Ba on Atlantic salmon ( Salmo salar ) larvae. 71% and 100% mark success were achieved after 1 h of immersion for 86Sr (75 µg L−1) and 137Ba (30 µg L−1) isotopes, respectively. Compared with conventional immersion, osmotic induction improved overall mark strength for 86Sr and 137Ba isotopes by 26–116%, although this effect was only observed after 12 h of immersion and predominately for 86Sr. The results demonstrate that osmotic induction reduces immersion times and the concentrations of isotope required to achieve successful marks. Osmotically induced isotope labels via larval immersion may prove a rapid and cost-effective way of batch marking fish larvae across a range of potential applications. |
format |
Text |
author |
de Braux, Emmanuel Warren-Myers, Fletcher Dempster, Tim Fjelldal, Per Gunnar Hansen, Tom Swearer, Stephen E. |
author_facet |
de Braux, Emmanuel Warren-Myers, Fletcher Dempster, Tim Fjelldal, Per Gunnar Hansen, Tom Swearer, Stephen E. |
author_sort |
de Braux, Emmanuel |
title |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_short |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_full |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_fullStr |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_full_unstemmed |
Osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
title_sort |
osmotic induction improves batch marking of larval fish otoliths with enriched stable isotopes |
publisher |
Oxford University Press |
publishDate |
2014 |
url |
http://icesjms.oxfordjournals.org/cgi/content/short/71/9/2530 https://doi.org/10.1093/icesjms/fsu091 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://icesjms.oxfordjournals.org/cgi/content/short/71/9/2530 http://dx.doi.org/10.1093/icesjms/fsu091 |
op_rights |
Copyright (C) 2014, International Council for the Exploration of the Sea/Conseil International pour l'Exploration de la Mer |
op_doi |
https://doi.org/10.1093/icesjms/fsu091 |
container_title |
ICES Journal of Marine Science |
container_volume |
71 |
container_issue |
9 |
container_start_page |
2530 |
op_container_end_page |
2538 |
_version_ |
1766362951467401216 |