Ageing octopods from stylets: development of a technique for permanent preparations
<qd> Barratt, I. M., and Allcock, A. L. 2010. Ageing octopods from stylets: development of a technique for permanent preparations. – ICES Journal of Marine Science, 67: 1452–1457. </qd>Previous attempts at ageing octopods from stylets have relied on preparations that deteriorate with tim...
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fthighwire:oai:open-archive.highwire.org:icesjms:67/7/1452 2023-05-15T14:02:57+02:00 Ageing octopods from stylets: development of a technique for permanent preparations Barratt, Iain M. Allcock, A. Louise 2010-10-01 00:00:00.0 text/html http://icesjms.oxfordjournals.org/cgi/content/short/67/7/1452 https://doi.org/10.1093/icesjms/fsq047 en eng Oxford University Press http://icesjms.oxfordjournals.org/cgi/content/short/67/7/1452 http://dx.doi.org/10.1093/icesjms/fsq047 Copyright (C) 2010, International Council for the Exploration of the Sea/Conseil International pour l'Exploration de la Mer Articles TEXT 2010 fthighwire https://doi.org/10.1093/icesjms/fsq047 2010-09-18T15:45:24Z <qd> Barratt, I. M., and Allcock, A. L. 2010. Ageing octopods from stylets: development of a technique for permanent preparations. – ICES Journal of Marine Science, 67: 1452–1457. </qd>Previous attempts at ageing octopods from stylets have relied on preparations that deteriorate with time. Some techniques require an immediate photographic record, others allow real-time enumeration but do not provide a permanent archive. A technique is described that produces permanent and archivable preparations of octopod stylets. Stylets were dehydrated in ethanol and infiltrated with a low-viscosity resin. Subsequent polymerization of the resin allowed the embedded stylet to be ground and polished to reveal the stylet microstructure. This comprised increments that are probably suitable for age estimation. The technique was developed using stylets of Octopus vulgaris and Eledone cirrhosa . Increments were composed of light and dark bands and were clearly defined at ×400 and at ×625 magnifications. The number of increments ranged from 189 to 399. The stylets of a deep-sea species (Bathypolypus sponsalis ) and an Antarctic species ( Megaleledone setebos ) were also examined. Each appeared to have growth increments, despite the perception that the environments they inhabited may not provide daily cues. Using the technique developed, the pre-hatch nucleus was seldom well defined, as reported for O. pallidus , stylets of which were prepared using a non-permanent method. Reasons for this are discussed. The microstructure clarity revealed is probably associated with the ultra-low viscosity of the resin used. Text Antarc* Antarctic Megaleledone Setebos HighWire Press (Stanford University) Antarctic ICES Journal of Marine Science 67 7 1452 1457 |
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Articles Barratt, Iain M. Allcock, A. Louise Ageing octopods from stylets: development of a technique for permanent preparations |
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<qd> Barratt, I. M., and Allcock, A. L. 2010. Ageing octopods from stylets: development of a technique for permanent preparations. – ICES Journal of Marine Science, 67: 1452–1457. </qd>Previous attempts at ageing octopods from stylets have relied on preparations that deteriorate with time. Some techniques require an immediate photographic record, others allow real-time enumeration but do not provide a permanent archive. A technique is described that produces permanent and archivable preparations of octopod stylets. Stylets were dehydrated in ethanol and infiltrated with a low-viscosity resin. Subsequent polymerization of the resin allowed the embedded stylet to be ground and polished to reveal the stylet microstructure. This comprised increments that are probably suitable for age estimation. The technique was developed using stylets of Octopus vulgaris and Eledone cirrhosa . Increments were composed of light and dark bands and were clearly defined at ×400 and at ×625 magnifications. The number of increments ranged from 189 to 399. The stylets of a deep-sea species (Bathypolypus sponsalis ) and an Antarctic species ( Megaleledone setebos ) were also examined. Each appeared to have growth increments, despite the perception that the environments they inhabited may not provide daily cues. Using the technique developed, the pre-hatch nucleus was seldom well defined, as reported for O. pallidus , stylets of which were prepared using a non-permanent method. Reasons for this are discussed. The microstructure clarity revealed is probably associated with the ultra-low viscosity of the resin used. |
format |
Text |
author |
Barratt, Iain M. Allcock, A. Louise |
author_facet |
Barratt, Iain M. Allcock, A. Louise |
author_sort |
Barratt, Iain M. |
title |
Ageing octopods from stylets: development of a technique for permanent preparations |
title_short |
Ageing octopods from stylets: development of a technique for permanent preparations |
title_full |
Ageing octopods from stylets: development of a technique for permanent preparations |
title_fullStr |
Ageing octopods from stylets: development of a technique for permanent preparations |
title_full_unstemmed |
Ageing octopods from stylets: development of a technique for permanent preparations |
title_sort |
ageing octopods from stylets: development of a technique for permanent preparations |
publisher |
Oxford University Press |
publishDate |
2010 |
url |
http://icesjms.oxfordjournals.org/cgi/content/short/67/7/1452 https://doi.org/10.1093/icesjms/fsq047 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Megaleledone Setebos |
genre_facet |
Antarc* Antarctic Megaleledone Setebos |
op_relation |
http://icesjms.oxfordjournals.org/cgi/content/short/67/7/1452 http://dx.doi.org/10.1093/icesjms/fsq047 |
op_rights |
Copyright (C) 2010, International Council for the Exploration of the Sea/Conseil International pour l'Exploration de la Mer |
op_doi |
https://doi.org/10.1093/icesjms/fsq047 |
container_title |
ICES Journal of Marine Science |
container_volume |
67 |
container_issue |
7 |
container_start_page |
1452 |
op_container_end_page |
1457 |
_version_ |
1766273408148963328 |