RAPD typing of north-east Atlantic minke whale (Balaenoptera acutorostrata)
This work describes the conditions for applying the arbitrary amplification of polymorphic DNA for stock determination in the minke whale. Out of 192 primers screened, only 36 were useful to detect genomic DNA-polymorphisms. Seven of these primers were used to type 31 minke whales caught in the wate...
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1997
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fthighwire:oai:open-archive.highwire.org:icesjms:54/3/478 2023-05-15T15:36:08+02:00 RAPD typing of north-east Atlantic minke whale (Balaenoptera acutorostrata) Martinez, I. Elvevoll, E. O. Haug, T. 1997-06-01 00:00:00.0 text/html http://icesjms.oxfordjournals.org/cgi/content/short/54/3/478 https://doi.org/10.1006/jmsc.1996.0168 en eng Oxford University Press http://icesjms.oxfordjournals.org/cgi/content/short/54/3/478 http://dx.doi.org/10.1006/jmsc.1996.0168 Copyright (C) 1997, International Council for the Exploration of the Sea/Conseil International pour l'Exploration de la Mer Articles TEXT 1997 fthighwire https://doi.org/10.1006/jmsc.1996.0168 2013-05-27T06:34:03Z This work describes the conditions for applying the arbitrary amplification of polymorphic DNA for stock determination in the minke whale. Out of 192 primers screened, only 36 were useful to detect genomic DNA-polymorphisms. Seven of these primers were used to type 31 minke whales caught in the waters around Spitsbergen (n=7), Bear Island (n=8), Finnmark (n=8) and Lofoten-Vesterålen (n=8). Our results showed first a low percentage of primers (19%) revealing polymorphisms, secondly a high number of polymorphic bands with high frequencies (6 out of 14 had a frequency higher than 0.77 and 3 of those had a frequency higher than 0.90) and thirdly an average fraction of matching bands that was sometimes greater between than within areas. None of these findings support the view that the 31 typed animals belonged to distinct breeding stocks, thus emphasizing the importance of carrying out a broader study including more animals, locations, years and techniques of analysis, in order to clarify the number and borderlines of breeding stocks in the north-east Atlantic. Text Balaenoptera acutorostrata Bear Island Finnmark Lofoten minke whale North East Atlantic Vesterålen Finnmark Spitsbergen HighWire Press (Stanford University) Bear Island ENVELOPE(-67.250,-67.250,-68.151,-68.151) Lofoten Vesterålen ENVELOPE(14.939,14.939,68.754,68.754) ICES Journal of Marine Science 54 3 478 484 |
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English |
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Articles |
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Articles Martinez, I. Elvevoll, E. O. Haug, T. RAPD typing of north-east Atlantic minke whale (Balaenoptera acutorostrata) |
topic_facet |
Articles |
description |
This work describes the conditions for applying the arbitrary amplification of polymorphic DNA for stock determination in the minke whale. Out of 192 primers screened, only 36 were useful to detect genomic DNA-polymorphisms. Seven of these primers were used to type 31 minke whales caught in the waters around Spitsbergen (n=7), Bear Island (n=8), Finnmark (n=8) and Lofoten-Vesterålen (n=8). Our results showed first a low percentage of primers (19%) revealing polymorphisms, secondly a high number of polymorphic bands with high frequencies (6 out of 14 had a frequency higher than 0.77 and 3 of those had a frequency higher than 0.90) and thirdly an average fraction of matching bands that was sometimes greater between than within areas. None of these findings support the view that the 31 typed animals belonged to distinct breeding stocks, thus emphasizing the importance of carrying out a broader study including more animals, locations, years and techniques of analysis, in order to clarify the number and borderlines of breeding stocks in the north-east Atlantic. |
format |
Text |
author |
Martinez, I. Elvevoll, E. O. Haug, T. |
author_facet |
Martinez, I. Elvevoll, E. O. Haug, T. |
author_sort |
Martinez, I. |
title |
RAPD typing of north-east Atlantic minke whale (Balaenoptera acutorostrata) |
title_short |
RAPD typing of north-east Atlantic minke whale (Balaenoptera acutorostrata) |
title_full |
RAPD typing of north-east Atlantic minke whale (Balaenoptera acutorostrata) |
title_fullStr |
RAPD typing of north-east Atlantic minke whale (Balaenoptera acutorostrata) |
title_full_unstemmed |
RAPD typing of north-east Atlantic minke whale (Balaenoptera acutorostrata) |
title_sort |
rapd typing of north-east atlantic minke whale (balaenoptera acutorostrata) |
publisher |
Oxford University Press |
publishDate |
1997 |
url |
http://icesjms.oxfordjournals.org/cgi/content/short/54/3/478 https://doi.org/10.1006/jmsc.1996.0168 |
long_lat |
ENVELOPE(-67.250,-67.250,-68.151,-68.151) ENVELOPE(14.939,14.939,68.754,68.754) |
geographic |
Bear Island Lofoten Vesterålen |
geographic_facet |
Bear Island Lofoten Vesterålen |
genre |
Balaenoptera acutorostrata Bear Island Finnmark Lofoten minke whale North East Atlantic Vesterålen Finnmark Spitsbergen |
genre_facet |
Balaenoptera acutorostrata Bear Island Finnmark Lofoten minke whale North East Atlantic Vesterålen Finnmark Spitsbergen |
op_relation |
http://icesjms.oxfordjournals.org/cgi/content/short/54/3/478 http://dx.doi.org/10.1006/jmsc.1996.0168 |
op_rights |
Copyright (C) 1997, International Council for the Exploration of the Sea/Conseil International pour l'Exploration de la Mer |
op_doi |
https://doi.org/10.1006/jmsc.1996.0168 |
container_title |
ICES Journal of Marine Science |
container_volume |
54 |
container_issue |
3 |
container_start_page |
478 |
op_container_end_page |
484 |
_version_ |
1766366468504551424 |