Adaptive Evolution of Gene Expression in Antarctic Fishes: Divergent Transcription of the 5'-to-5' Linked Adult {alpha}1- and {beta}-Globin Genes of the Antarctic Teleost Notothenia coriiceps is Controlled by Dual Promoters and Intergenic Enhancers
Unlike temperate fishes, Antarctic fishes of the notothenioid suborder, whose body temperatures (−2 to +1°C) conform to the Southern Ocean, must express their genomes in an extremely cold thermal regime. To determine whether these fishes have evolved compensatory adjustments that maintain efficient...
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2001
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fthighwire:oai:open-archive.highwire.org:icbiol:41/1/113 2023-05-15T14:03:07+02:00 Adaptive Evolution of Gene Expression in Antarctic Fishes: Divergent Transcription of the 5'-to-5' Linked Adult {alpha}1- and {beta}-Globin Genes of the Antarctic Teleost Notothenia coriiceps is Controlled by Dual Promoters and Intergenic Enhancers Lau, David T. Saeed-Kothe, Amna Parker, Sandra K. William Detrich, H. 2001-02-01 00:00:00.0 text/html http://icb.oxfordjournals.org/cgi/content/short/41/1/113 https://doi.org/10.1093/icb/41.1.113 en eng Oxford University Press http://icb.oxfordjournals.org/cgi/content/short/41/1/113 http://dx.doi.org/10.1093/icb/41.1.113 Copyright (C) 2001, The Society for Integrative and Comparative Biology Regular Article TEXT 2001 fthighwire https://doi.org/10.1093/icb/41.1.113 2007-06-24T22:08:17Z Unlike temperate fishes, Antarctic fishes of the notothenioid suborder, whose body temperatures (−2 to +1°C) conform to the Southern Ocean, must express their genomes in an extremely cold thermal regime. To determine whether these fishes have evolved compensatory adjustments that maintain efficient gene transcription at low temperatures, we have initiated studies of the cis -acting regulatory elements that control globin gene expression in the Antarctic rockcod Notothenia coriiceps and in its close relative, the temperate New Zealand black cod N. angustata (habitat temperature = +6 to +15°C). The genes encoding the major α1 and β globins of these fishes are tightly linked in head-to-head (5′ to 5′) orientation. The intergenic regions separating the globin genes in the two fishes, ∼4.3 kb in N. coriiceps and ∼3.2 kb in N. angustata , are highly similar in sequence, the major difference being the absence of a 1.1-kb, repeat-containing segment in the latter. To assess the promoter and enhancer activities of the intergenic regions, each was cloned into the luciferase-reporter vector pGL3-Basic, and the constructs were transfected into MEL cells. Upon DMSO induction of MEL cell differentiation, each of the α/β-intergenic regions functioned in both orientations as erythroid-responsive transcriptional regulators. However, expression of luciferase mediated by the N. coriiceps intergene was 6-fold greater in the α orientation than that for the N. angustata intergene and 2-fold greater for the β. The greater transcription-stimulating activity of the N. coriiceps intergene can be attributed to two enhancers composed of combinations of CAC/Sp1 and GATA motifs and located in direct repeat elements. N. angustata , which lacked repetitive structure in its intergene, contained a single copy of the enhancer. We propose that cold adaptation of globin gene expression in N. coriiceps evolved in part through duplication and refinement of critical cis -acting regulatory elements as the Southern Ocean cooled during the past 25 million ... Text Antarc* Antarctic Southern Ocean HighWire Press (Stanford University) Antarctic Gata ENVELOPE(-19.702,-19.702,63.540,63.540) New Zealand Southern Ocean The Antarctic American Zoologist 41 1 113 132 |
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HighWire Press (Stanford University) |
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English |
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Regular Article |
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Regular Article Lau, David T. Saeed-Kothe, Amna Parker, Sandra K. William Detrich, H. Adaptive Evolution of Gene Expression in Antarctic Fishes: Divergent Transcription of the 5'-to-5' Linked Adult {alpha}1- and {beta}-Globin Genes of the Antarctic Teleost Notothenia coriiceps is Controlled by Dual Promoters and Intergenic Enhancers |
topic_facet |
Regular Article |
description |
Unlike temperate fishes, Antarctic fishes of the notothenioid suborder, whose body temperatures (−2 to +1°C) conform to the Southern Ocean, must express their genomes in an extremely cold thermal regime. To determine whether these fishes have evolved compensatory adjustments that maintain efficient gene transcription at low temperatures, we have initiated studies of the cis -acting regulatory elements that control globin gene expression in the Antarctic rockcod Notothenia coriiceps and in its close relative, the temperate New Zealand black cod N. angustata (habitat temperature = +6 to +15°C). The genes encoding the major α1 and β globins of these fishes are tightly linked in head-to-head (5′ to 5′) orientation. The intergenic regions separating the globin genes in the two fishes, ∼4.3 kb in N. coriiceps and ∼3.2 kb in N. angustata , are highly similar in sequence, the major difference being the absence of a 1.1-kb, repeat-containing segment in the latter. To assess the promoter and enhancer activities of the intergenic regions, each was cloned into the luciferase-reporter vector pGL3-Basic, and the constructs were transfected into MEL cells. Upon DMSO induction of MEL cell differentiation, each of the α/β-intergenic regions functioned in both orientations as erythroid-responsive transcriptional regulators. However, expression of luciferase mediated by the N. coriiceps intergene was 6-fold greater in the α orientation than that for the N. angustata intergene and 2-fold greater for the β. The greater transcription-stimulating activity of the N. coriiceps intergene can be attributed to two enhancers composed of combinations of CAC/Sp1 and GATA motifs and located in direct repeat elements. N. angustata , which lacked repetitive structure in its intergene, contained a single copy of the enhancer. We propose that cold adaptation of globin gene expression in N. coriiceps evolved in part through duplication and refinement of critical cis -acting regulatory elements as the Southern Ocean cooled during the past 25 million ... |
format |
Text |
author |
Lau, David T. Saeed-Kothe, Amna Parker, Sandra K. William Detrich, H. |
author_facet |
Lau, David T. Saeed-Kothe, Amna Parker, Sandra K. William Detrich, H. |
author_sort |
Lau, David T. |
title |
Adaptive Evolution of Gene Expression in Antarctic Fishes: Divergent Transcription of the 5'-to-5' Linked Adult {alpha}1- and {beta}-Globin Genes of the Antarctic Teleost Notothenia coriiceps is Controlled by Dual Promoters and Intergenic Enhancers |
title_short |
Adaptive Evolution of Gene Expression in Antarctic Fishes: Divergent Transcription of the 5'-to-5' Linked Adult {alpha}1- and {beta}-Globin Genes of the Antarctic Teleost Notothenia coriiceps is Controlled by Dual Promoters and Intergenic Enhancers |
title_full |
Adaptive Evolution of Gene Expression in Antarctic Fishes: Divergent Transcription of the 5'-to-5' Linked Adult {alpha}1- and {beta}-Globin Genes of the Antarctic Teleost Notothenia coriiceps is Controlled by Dual Promoters and Intergenic Enhancers |
title_fullStr |
Adaptive Evolution of Gene Expression in Antarctic Fishes: Divergent Transcription of the 5'-to-5' Linked Adult {alpha}1- and {beta}-Globin Genes of the Antarctic Teleost Notothenia coriiceps is Controlled by Dual Promoters and Intergenic Enhancers |
title_full_unstemmed |
Adaptive Evolution of Gene Expression in Antarctic Fishes: Divergent Transcription of the 5'-to-5' Linked Adult {alpha}1- and {beta}-Globin Genes of the Antarctic Teleost Notothenia coriiceps is Controlled by Dual Promoters and Intergenic Enhancers |
title_sort |
adaptive evolution of gene expression in antarctic fishes: divergent transcription of the 5'-to-5' linked adult {alpha}1- and {beta}-globin genes of the antarctic teleost notothenia coriiceps is controlled by dual promoters and intergenic enhancers |
publisher |
Oxford University Press |
publishDate |
2001 |
url |
http://icb.oxfordjournals.org/cgi/content/short/41/1/113 https://doi.org/10.1093/icb/41.1.113 |
long_lat |
ENVELOPE(-19.702,-19.702,63.540,63.540) |
geographic |
Antarctic Gata New Zealand Southern Ocean The Antarctic |
geographic_facet |
Antarctic Gata New Zealand Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
http://icb.oxfordjournals.org/cgi/content/short/41/1/113 http://dx.doi.org/10.1093/icb/41.1.113 |
op_rights |
Copyright (C) 2001, The Society for Integrative and Comparative Biology |
op_doi |
https://doi.org/10.1093/icb/41.1.113 |
container_title |
American Zoologist |
container_volume |
41 |
container_issue |
1 |
container_start_page |
113 |
op_container_end_page |
132 |
_version_ |
1766273646170472448 |