The role of PR-Set7 in replication licensing depends on Suv4-20h
PR-Set7 is the sole monomethyltransferase responsible for H4K20 monomethylation (H4K20me1) that is the substrate for further methylation by Suv4-20h1/h2. PR-Set7 is required for proper cell cycle progression and is subject to degradation by the CRL4Cdt2 ubiquitin ligase complex as a function of the...
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2012
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fthighwire:oai:open-archive.highwire.org:genesdev:gad.195636.112v1 2023-05-15T17:53:50+02:00 The role of PR-Set7 in replication licensing depends on Suv4-20h Beck, David B. Burton, Adam Oda, Hisanobu Ziegler-Birling, Céline Torres-Padilla, Maria-Elena Reinberg, Danny 2012-11-14 07:00:43.0 text/html http://genesdev.cshlp.org/cgi/content/short/gad.195636.112v1 https://doi.org/10.1101/gad.195636.112 en eng Cold Spring Harbor Laboratory Press http://genesdev.cshlp.org/cgi/content/short/gad.195636.112v1 http://dx.doi.org/10.1101/gad.195636.112 Copyright (C) 2012, Cold Spring Harbor Laboratory Press Research Papers TEXT 2012 fthighwire https://doi.org/10.1101/gad.195636.112 2013-05-27T21:04:19Z PR-Set7 is the sole monomethyltransferase responsible for H4K20 monomethylation (H4K20me1) that is the substrate for further methylation by Suv4-20h1/h2. PR-Set7 is required for proper cell cycle progression and is subject to degradation by the CRL4Cdt2 ubiquitin ligase complex as a function of the cell cycle and DNA damage. This report demonstrates that PR-Set7 is an important downstream effector of CRL4Cdt2 function during origin of DNA replication licensing, dependent on Suv4-20h1/2 activity. Aberrant rereplication correlates with decreased levels of H4K20me1 and increased levels of H4K20 trimethylation (H4K20me3). Expression of a degradation-resistant PR-Set7 mutant in the mouse embryo that is normally devoid of Suv4-20 does not compromise development or cell cycle progression unless Suv4-20h is coexpressed. PR-Set7 targeting to an artificial locus results in recruitment of the origin recognition complex (ORC) in a manner dependent on Suv4-20h and H4K20me3. Consistent with this, H4K20 methylation status plays a direct role in recruiting ORC through the binding properties of ORC1 and ORCA/LRWD1. Thus, coordinating the status of H4K20 methylation is pivotal for the proper selection of DNA replication origins in higher eukaryotes. Text Orca HighWire Press (Stanford University) Genes & Development 26 23 2580 2589 |
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Research Papers Beck, David B. Burton, Adam Oda, Hisanobu Ziegler-Birling, Céline Torres-Padilla, Maria-Elena Reinberg, Danny The role of PR-Set7 in replication licensing depends on Suv4-20h |
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Research Papers |
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PR-Set7 is the sole monomethyltransferase responsible for H4K20 monomethylation (H4K20me1) that is the substrate for further methylation by Suv4-20h1/h2. PR-Set7 is required for proper cell cycle progression and is subject to degradation by the CRL4Cdt2 ubiquitin ligase complex as a function of the cell cycle and DNA damage. This report demonstrates that PR-Set7 is an important downstream effector of CRL4Cdt2 function during origin of DNA replication licensing, dependent on Suv4-20h1/2 activity. Aberrant rereplication correlates with decreased levels of H4K20me1 and increased levels of H4K20 trimethylation (H4K20me3). Expression of a degradation-resistant PR-Set7 mutant in the mouse embryo that is normally devoid of Suv4-20 does not compromise development or cell cycle progression unless Suv4-20h is coexpressed. PR-Set7 targeting to an artificial locus results in recruitment of the origin recognition complex (ORC) in a manner dependent on Suv4-20h and H4K20me3. Consistent with this, H4K20 methylation status plays a direct role in recruiting ORC through the binding properties of ORC1 and ORCA/LRWD1. Thus, coordinating the status of H4K20 methylation is pivotal for the proper selection of DNA replication origins in higher eukaryotes. |
format |
Text |
author |
Beck, David B. Burton, Adam Oda, Hisanobu Ziegler-Birling, Céline Torres-Padilla, Maria-Elena Reinberg, Danny |
author_facet |
Beck, David B. Burton, Adam Oda, Hisanobu Ziegler-Birling, Céline Torres-Padilla, Maria-Elena Reinberg, Danny |
author_sort |
Beck, David B. |
title |
The role of PR-Set7 in replication licensing depends on Suv4-20h |
title_short |
The role of PR-Set7 in replication licensing depends on Suv4-20h |
title_full |
The role of PR-Set7 in replication licensing depends on Suv4-20h |
title_fullStr |
The role of PR-Set7 in replication licensing depends on Suv4-20h |
title_full_unstemmed |
The role of PR-Set7 in replication licensing depends on Suv4-20h |
title_sort |
role of pr-set7 in replication licensing depends on suv4-20h |
publisher |
Cold Spring Harbor Laboratory Press |
publishDate |
2012 |
url |
http://genesdev.cshlp.org/cgi/content/short/gad.195636.112v1 https://doi.org/10.1101/gad.195636.112 |
genre |
Orca |
genre_facet |
Orca |
op_relation |
http://genesdev.cshlp.org/cgi/content/short/gad.195636.112v1 http://dx.doi.org/10.1101/gad.195636.112 |
op_rights |
Copyright (C) 2012, Cold Spring Harbor Laboratory Press |
op_doi |
https://doi.org/10.1101/gad.195636.112 |
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Genes & Development |
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26 |
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23 |
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2580 |
op_container_end_page |
2589 |
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1766161534845714432 |