Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos.
qmf1 is a quail myogenic regulatory gene that is transcribed in skeletal myoblasts and differentiated muscle and shows sequence homology to MyoD1 and Myf5. We used the qmf1 transcript as an in situ hybridization marker for determined myogenic cells to study myogenic lineages in developing embryos. W...
Published in: | Genes & Development |
---|---|
Main Authors: | , |
Format: | Text |
Language: | English |
Published: |
Cold Spring Harbor Laboratory Press
1990
|
Subjects: | |
Online Access: | http://genesdev.cshlp.org/cgi/content/short/4/4/567 https://doi.org/10.1101/gad.4.4.567 |
id |
fthighwire:oai:open-archive.highwire.org:genesdev:4/4/567 |
---|---|
record_format |
openpolar |
spelling |
fthighwire:oai:open-archive.highwire.org:genesdev:4/4/567 2023-05-15T16:01:33+02:00 Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos. de la Brousse, F C Emerson, C P 1990-04-01 00:00:00.0 text/html http://genesdev.cshlp.org/cgi/content/short/4/4/567 https://doi.org/10.1101/gad.4.4.567 en eng Cold Spring Harbor Laboratory Press http://genesdev.cshlp.org/cgi/content/short/4/4/567 http://dx.doi.org/10.1101/gad.4.4.567 Copyright (C) 1990, Cold Spring Harbor Laboratory Press Research Papers TEXT 1990 fthighwire https://doi.org/10.1101/gad.4.4.567 2013-05-27T17:38:03Z qmf1 is a quail myogenic regulatory gene that is transcribed in skeletal myoblasts and differentiated muscle and shows sequence homology to MyoD1 and Myf5. We used the qmf1 transcript as an in situ hybridization marker for determined myogenic cells to study myogenic lineages in developing embryos. We present evidence for the temporal and spatial regulation of qmf1 mRNA expression and slow cardiac troponin C (TnC), fast skeletal troponin T (TnT), and alpha-cardiac actin contractile protein mRNA expression in the somite myotome and limb buds. Our results show that qmf1 is a marker for myogenic lineages during both somite formation and limb development and that qmf1 mRNAs, but not contractile protein mRNAs, localize in dorsal medial lip (DML) cells of the somite dermatome. We propose that the DML is a site of myogenic lineage determination. Text DML HighWire Press (Stanford University) Genes & Development 4 4 567 581 |
institution |
Open Polar |
collection |
HighWire Press (Stanford University) |
op_collection_id |
fthighwire |
language |
English |
topic |
Research Papers |
spellingShingle |
Research Papers de la Brousse, F C Emerson, C P Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos. |
topic_facet |
Research Papers |
description |
qmf1 is a quail myogenic regulatory gene that is transcribed in skeletal myoblasts and differentiated muscle and shows sequence homology to MyoD1 and Myf5. We used the qmf1 transcript as an in situ hybridization marker for determined myogenic cells to study myogenic lineages in developing embryos. We present evidence for the temporal and spatial regulation of qmf1 mRNA expression and slow cardiac troponin C (TnC), fast skeletal troponin T (TnT), and alpha-cardiac actin contractile protein mRNA expression in the somite myotome and limb buds. Our results show that qmf1 is a marker for myogenic lineages during both somite formation and limb development and that qmf1 mRNAs, but not contractile protein mRNAs, localize in dorsal medial lip (DML) cells of the somite dermatome. We propose that the DML is a site of myogenic lineage determination. |
format |
Text |
author |
de la Brousse, F C Emerson, C P |
author_facet |
de la Brousse, F C Emerson, C P |
author_sort |
de la Brousse, F C |
title |
Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos. |
title_short |
Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos. |
title_full |
Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos. |
title_fullStr |
Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos. |
title_full_unstemmed |
Localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos. |
title_sort |
localized expression of a myogenic regulatory gene, qmf1, in the somite dermatome of avian embryos. |
publisher |
Cold Spring Harbor Laboratory Press |
publishDate |
1990 |
url |
http://genesdev.cshlp.org/cgi/content/short/4/4/567 https://doi.org/10.1101/gad.4.4.567 |
genre |
DML |
genre_facet |
DML |
op_relation |
http://genesdev.cshlp.org/cgi/content/short/4/4/567 http://dx.doi.org/10.1101/gad.4.4.567 |
op_rights |
Copyright (C) 1990, Cold Spring Harbor Laboratory Press |
op_doi |
https://doi.org/10.1101/gad.4.4.567 |
container_title |
Genes & Development |
container_volume |
4 |
container_issue |
4 |
container_start_page |
567 |
op_container_end_page |
581 |
_version_ |
1766397354511958016 |