Genetic analysis of turbot pathogenic Streptococcus parauberis strains by ribotyping and random amplified polymorphic DNA

Ribotyping and RAPD profiling of a collection of 18 Streptococcus parauberis strains isolated from diseased turbot in Galicia (NW Spain) was performed in order to analyze the possible genetic variability within this bacterial fish pathogen. In addition, the value of this technique for intraspecific...

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Bibliographic Details
Published in:FEMS Microbiology Letters
Main Authors: Romalde, J.L., Magariños, B., Villar, C., Barja, J.L., Toranzo, A.E.
Format: Text
Language:English
Published: Oxford University Press 1999
Subjects:
Articles
Turbot
Online Access:http://femsle.oxfordjournals.org/cgi/content/short/179/2/297
https://doi.org/10.1111/j.1574-6968.1999.tb08741.x
Description
Summary:Ribotyping and RAPD profiling of a collection of 18 Streptococcus parauberis strains isolated from diseased turbot in Galicia (NW Spain) was performed in order to analyze the possible genetic variability within this bacterial fish pathogen. In addition, the value of this technique for intraspecific classification and epidemiological studies was evaluated. Ribopatterns of DNA digested with three endonucleases and hybridized with a cDNA probe complementary to highly conserved sequences in the 16S and 23S rRNA genes showed a great homogeneity among the turbot isolates. Compared with ribotyping, RAPD appeared to be a reliable and fast technique for discriminating between isolates of S. parauberis on the basis of their farm of isolation and, therefore, represents a powerful tool for epidemiological studies of this fish pathogen.

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