A novel mechanism of urease regulation in Yersinia enterocolitica
Yersinia enterocolitica produces the enzyme urease which hydrolyses urea, resulting in the production of carbonic acid and ammonia and a net increase in pH. In the presence of urea, urease enhances survival of Y. enterocolitica in the stomach and presumably in other acidic environments the bacteria...
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1997
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fthighwire:oai:open-archive.highwire.org:femsle:147/2/221 2023-05-15T15:52:46+02:00 A novel mechanism of urease regulation in Yersinia enterocolitica De Koning-Ward, Tania F Robins-Browne, Roy M 1997-02-01 00:00:00.0 text/html http://femsle.oxfordjournals.org/cgi/content/short/147/2/221 https://doi.org/10.1111/j.1574-6968.1997.tb10245.x en eng Oxford University Press http://femsle.oxfordjournals.org/cgi/content/short/147/2/221 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10245.x Copyright (C) 1997, Oxford University Press Articles TEXT 1997 fthighwire https://doi.org/10.1111/j.1574-6968.1997.tb10245.x 2015-02-28T22:26:09Z Yersinia enterocolitica produces the enzyme urease which hydrolyses urea, resulting in the production of carbonic acid and ammonia and a net increase in pH. In the presence of urea, urease enhances survival of Y. enterocolitica in the stomach and presumably in other acidic environments the bacteria encounter during the course of infection. In this study we show that Y. enterocolitica urease is a cytosolic enzyme which has a low K m value (0.15±0.01 mM urea), suggesting that it functions at close to maximum velocity even at the low concentrations of urea available to Y. enterocolitica in gastric fluid and other tissues. Y. enterocolitica urease was active over a wide pH range, but unlike most other bacterial ureases, displayed an optimal activity at pH 3.5–4.5, suggesting a physiological role in protecting the bacteria from acid. Higher levels of urease activity were attained at 28°C than at 37°C, and investigation of the regulation of urease production revealed that the enzyme was not induced by urea, or by nitrogen limitation. Instead maximal activity was attained during the stationary phase of growth which coincides with the period of maximum acid tolerance of the bacteria. This type of regulation has not been described for any other ureolytic bacteria and seems to be unique to Y. enterocolitica . Text Carbonic acid HighWire Press (Stanford University) FEMS Microbiology Letters 147 2 221 226 |
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Articles De Koning-Ward, Tania F Robins-Browne, Roy M A novel mechanism of urease regulation in Yersinia enterocolitica |
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Yersinia enterocolitica produces the enzyme urease which hydrolyses urea, resulting in the production of carbonic acid and ammonia and a net increase in pH. In the presence of urea, urease enhances survival of Y. enterocolitica in the stomach and presumably in other acidic environments the bacteria encounter during the course of infection. In this study we show that Y. enterocolitica urease is a cytosolic enzyme which has a low K m value (0.15±0.01 mM urea), suggesting that it functions at close to maximum velocity even at the low concentrations of urea available to Y. enterocolitica in gastric fluid and other tissues. Y. enterocolitica urease was active over a wide pH range, but unlike most other bacterial ureases, displayed an optimal activity at pH 3.5–4.5, suggesting a physiological role in protecting the bacteria from acid. Higher levels of urease activity were attained at 28°C than at 37°C, and investigation of the regulation of urease production revealed that the enzyme was not induced by urea, or by nitrogen limitation. Instead maximal activity was attained during the stationary phase of growth which coincides with the period of maximum acid tolerance of the bacteria. This type of regulation has not been described for any other ureolytic bacteria and seems to be unique to Y. enterocolitica . |
format |
Text |
author |
De Koning-Ward, Tania F Robins-Browne, Roy M |
author_facet |
De Koning-Ward, Tania F Robins-Browne, Roy M |
author_sort |
De Koning-Ward, Tania F |
title |
A novel mechanism of urease regulation in Yersinia enterocolitica |
title_short |
A novel mechanism of urease regulation in Yersinia enterocolitica |
title_full |
A novel mechanism of urease regulation in Yersinia enterocolitica |
title_fullStr |
A novel mechanism of urease regulation in Yersinia enterocolitica |
title_full_unstemmed |
A novel mechanism of urease regulation in Yersinia enterocolitica |
title_sort |
novel mechanism of urease regulation in yersinia enterocolitica |
publisher |
Oxford University Press |
publishDate |
1997 |
url |
http://femsle.oxfordjournals.org/cgi/content/short/147/2/221 https://doi.org/10.1111/j.1574-6968.1997.tb10245.x |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
http://femsle.oxfordjournals.org/cgi/content/short/147/2/221 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10245.x |
op_rights |
Copyright (C) 1997, Oxford University Press |
op_doi |
https://doi.org/10.1111/j.1574-6968.1997.tb10245.x |
container_title |
FEMS Microbiology Letters |
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147 |
container_issue |
2 |
container_start_page |
221 |
op_container_end_page |
226 |
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1766387866723680256 |