Adherence and invasive capacities of the fish pathogen Pasteurella piscicida
Pasteurella piscicida strains were weakly or moderately adherent to cell lines, the levels of attachment being variable depending on the cells employed. All the isolates exhibited the highest binding capacity to CHSE-214 cells. Adhesive capacities were affected by heat and sugars but not by proteina...
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1996
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fthighwire:oai:open-archive.highwire.org:femsle:138/1/29 2023-05-15T18:41:12+02:00 Adherence and invasive capacities of the fish pathogen Pasteurella piscicida Magariños, Beatriz Romalde, Jesus L. Noya, Manuel Barja, Juan L. Toranzo, Alicia E. 1996-04-01 00:00:00.0 text/html http://femsle.oxfordjournals.org/cgi/content/short/138/1/29 https://doi.org/10.1111/j.1574-6968.1996.tb08130.x en eng Oxford University Press http://femsle.oxfordjournals.org/cgi/content/short/138/1/29 http://dx.doi.org/10.1111/j.1574-6968.1996.tb08130.x Copyright (C) 1996, Oxford University Press Research Letter TEXT 1996 fthighwire https://doi.org/10.1111/j.1574-6968.1996.tb08130.x 2015-02-28T23:55:06Z Pasteurella piscicida strains were weakly or moderately adherent to cell lines, the levels of attachment being variable depending on the cells employed. All the isolates exhibited the highest binding capacity to CHSE-214 cells. Adhesive capacities were affected by heat and sugars but not by proteinase K or by treatment with antisera raised against the lipopolysaccharides of P. piscicida , implicating components of glycoprotein(s) as ligands in the adhesion process. The isolates showed a great binding capacity to intestines from the marine fish hosts gilthead sea bream, sea bass and turbot, with values ranging from 104 to 105 bacteria/g. Although the P. piscicida strains showed a weak invasiveness in the poikilothermic cell lines employed as in vitro model, the bacteria remained viable inside the infected cells at least for 2 days. The invasion process was inhibited by cytochalasin D indicating the active participation of the host cell cytoskeleton in the internalization of P. piscicida . Text Turbot HighWire Press (Stanford University) FEMS Microbiology Letters 138 1 29 34 |
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HighWire Press (Stanford University) |
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English |
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Research Letter |
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Research Letter Magariños, Beatriz Romalde, Jesus L. Noya, Manuel Barja, Juan L. Toranzo, Alicia E. Adherence and invasive capacities of the fish pathogen Pasteurella piscicida |
topic_facet |
Research Letter |
description |
Pasteurella piscicida strains were weakly or moderately adherent to cell lines, the levels of attachment being variable depending on the cells employed. All the isolates exhibited the highest binding capacity to CHSE-214 cells. Adhesive capacities were affected by heat and sugars but not by proteinase K or by treatment with antisera raised against the lipopolysaccharides of P. piscicida , implicating components of glycoprotein(s) as ligands in the adhesion process. The isolates showed a great binding capacity to intestines from the marine fish hosts gilthead sea bream, sea bass and turbot, with values ranging from 104 to 105 bacteria/g. Although the P. piscicida strains showed a weak invasiveness in the poikilothermic cell lines employed as in vitro model, the bacteria remained viable inside the infected cells at least for 2 days. The invasion process was inhibited by cytochalasin D indicating the active participation of the host cell cytoskeleton in the internalization of P. piscicida . |
format |
Text |
author |
Magariños, Beatriz Romalde, Jesus L. Noya, Manuel Barja, Juan L. Toranzo, Alicia E. |
author_facet |
Magariños, Beatriz Romalde, Jesus L. Noya, Manuel Barja, Juan L. Toranzo, Alicia E. |
author_sort |
Magariños, Beatriz |
title |
Adherence and invasive capacities of the fish pathogen Pasteurella piscicida |
title_short |
Adherence and invasive capacities of the fish pathogen Pasteurella piscicida |
title_full |
Adherence and invasive capacities of the fish pathogen Pasteurella piscicida |
title_fullStr |
Adherence and invasive capacities of the fish pathogen Pasteurella piscicida |
title_full_unstemmed |
Adherence and invasive capacities of the fish pathogen Pasteurella piscicida |
title_sort |
adherence and invasive capacities of the fish pathogen pasteurella piscicida |
publisher |
Oxford University Press |
publishDate |
1996 |
url |
http://femsle.oxfordjournals.org/cgi/content/short/138/1/29 https://doi.org/10.1111/j.1574-6968.1996.tb08130.x |
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Turbot |
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Turbot |
op_relation |
http://femsle.oxfordjournals.org/cgi/content/short/138/1/29 http://dx.doi.org/10.1111/j.1574-6968.1996.tb08130.x |
op_rights |
Copyright (C) 1996, Oxford University Press |
op_doi |
https://doi.org/10.1111/j.1574-6968.1996.tb08130.x |
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FEMS Microbiology Letters |
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138 |
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1 |
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29 |
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34 |
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1766230701575766016 |