Crude oil-induced structural shift of coastal bacterial communities of rod bay (Terra Nova Bay, Ross Sea, Antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria
For preliminary screening of human impact on Antarctic coasts, the compositions of microbial communities were analyzed in seawater at two sites located in the Terra Nova Bay of Antarctica (Ross Sea) by a combination of 16S rRNA gene sequencing and culture techniques. The bacterial community in the s...
Published in: | FEMS Microbiology Ecology |
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fthighwire:oai:open-archive.highwire.org:femsec:49/3/419 2023-05-15T13:58:04+02:00 Crude oil-induced structural shift of coastal bacterial communities of rod bay (Terra Nova Bay, Ross Sea, Antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria Yakimov, Michail M. Gentile, Gabriella Bruni, Vivia Cappello, Simone D'Auria, Giuseppe Golyshin, Peter N. Giuliano, Laura 2004-09-01 00:00:00.0 text/html http://femsec.oxfordjournals.org/cgi/content/short/49/3/419 https://doi.org/10.1016/j.femsec.2004.04.018 en eng Oxford University Press http://femsec.oxfordjournals.org/cgi/content/short/49/3/419 http://dx.doi.org/10.1016/j.femsec.2004.04.018 Copyright (C) 2004, Oxford University Press Articles TEXT 2004 fthighwire https://doi.org/10.1016/j.femsec.2004.04.018 2015-03-01T00:52:08Z For preliminary screening of human impact on Antarctic coasts, the compositions of microbial communities were analyzed in seawater at two sites located in the Terra Nova Bay of Antarctica (Ross Sea) by a combination of 16S rRNA gene sequencing and culture techniques. The bacterial community in the sample from the Rod Bay site, located at the proximity to the Italian Station, was characterized by a high abundance of 16S rRNA gene sequences belonging to the microflora typically found in soil and freshwater environments. In contrast, the seawater sample from the Adelie Cove station, a pristine reference site, contained 16S rRNA gene sequences typically found in marine areas affected by algal blooms and sea ice decay. The addition of crude oil to the Rod Bay seawater sample rapidly induced a shift in the composition of the bacterial community with appearance of novel taxonomic groups and a dramatic increase in the relative abundance of γ- Proteobacteria sequences, whereas no significant changes were detected in the bacterial community of the Adelie Cove sample under the same conditions. Bacteria-exhibiting features with potential interest for industrial and environmental applications were isolated from the Rod Bay oil-enriched sample. In particular, hydrocarbon-degrading, cold-adapted bacteria were selectively enriched, isolated and screened for their ability to synthesize polyunsaturated fatty acids. Twenty two bacterial strains were isolated from the oil enrichment culture and identified. Eighteen isolates were found to be members of γ- Proteobacteria , while the remainder were representatives of α- Proteobacteria , CFB and high G + C divisions. Text Antarc* Antarctic Antarctica Ross Sea Sea ice HighWire Press (Stanford University) Antarctic Ross Sea Terra Nova Bay FEMS Microbiology Ecology 49 3 419 432 |
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English |
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Articles Yakimov, Michail M. Gentile, Gabriella Bruni, Vivia Cappello, Simone D'Auria, Giuseppe Golyshin, Peter N. Giuliano, Laura Crude oil-induced structural shift of coastal bacterial communities of rod bay (Terra Nova Bay, Ross Sea, Antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria |
topic_facet |
Articles |
description |
For preliminary screening of human impact on Antarctic coasts, the compositions of microbial communities were analyzed in seawater at two sites located in the Terra Nova Bay of Antarctica (Ross Sea) by a combination of 16S rRNA gene sequencing and culture techniques. The bacterial community in the sample from the Rod Bay site, located at the proximity to the Italian Station, was characterized by a high abundance of 16S rRNA gene sequences belonging to the microflora typically found in soil and freshwater environments. In contrast, the seawater sample from the Adelie Cove station, a pristine reference site, contained 16S rRNA gene sequences typically found in marine areas affected by algal blooms and sea ice decay. The addition of crude oil to the Rod Bay seawater sample rapidly induced a shift in the composition of the bacterial community with appearance of novel taxonomic groups and a dramatic increase in the relative abundance of γ- Proteobacteria sequences, whereas no significant changes were detected in the bacterial community of the Adelie Cove sample under the same conditions. Bacteria-exhibiting features with potential interest for industrial and environmental applications were isolated from the Rod Bay oil-enriched sample. In particular, hydrocarbon-degrading, cold-adapted bacteria were selectively enriched, isolated and screened for their ability to synthesize polyunsaturated fatty acids. Twenty two bacterial strains were isolated from the oil enrichment culture and identified. Eighteen isolates were found to be members of γ- Proteobacteria , while the remainder were representatives of α- Proteobacteria , CFB and high G + C divisions. |
format |
Text |
author |
Yakimov, Michail M. Gentile, Gabriella Bruni, Vivia Cappello, Simone D'Auria, Giuseppe Golyshin, Peter N. Giuliano, Laura |
author_facet |
Yakimov, Michail M. Gentile, Gabriella Bruni, Vivia Cappello, Simone D'Auria, Giuseppe Golyshin, Peter N. Giuliano, Laura |
author_sort |
Yakimov, Michail M. |
title |
Crude oil-induced structural shift of coastal bacterial communities of rod bay (Terra Nova Bay, Ross Sea, Antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria |
title_short |
Crude oil-induced structural shift of coastal bacterial communities of rod bay (Terra Nova Bay, Ross Sea, Antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria |
title_full |
Crude oil-induced structural shift of coastal bacterial communities of rod bay (Terra Nova Bay, Ross Sea, Antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria |
title_fullStr |
Crude oil-induced structural shift of coastal bacterial communities of rod bay (Terra Nova Bay, Ross Sea, Antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria |
title_full_unstemmed |
Crude oil-induced structural shift of coastal bacterial communities of rod bay (Terra Nova Bay, Ross Sea, Antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria |
title_sort |
crude oil-induced structural shift of coastal bacterial communities of rod bay (terra nova bay, ross sea, antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria |
publisher |
Oxford University Press |
publishDate |
2004 |
url |
http://femsec.oxfordjournals.org/cgi/content/short/49/3/419 https://doi.org/10.1016/j.femsec.2004.04.018 |
geographic |
Antarctic Ross Sea Terra Nova Bay |
geographic_facet |
Antarctic Ross Sea Terra Nova Bay |
genre |
Antarc* Antarctic Antarctica Ross Sea Sea ice |
genre_facet |
Antarc* Antarctic Antarctica Ross Sea Sea ice |
op_relation |
http://femsec.oxfordjournals.org/cgi/content/short/49/3/419 http://dx.doi.org/10.1016/j.femsec.2004.04.018 |
op_rights |
Copyright (C) 2004, Oxford University Press |
op_doi |
https://doi.org/10.1016/j.femsec.2004.04.018 |
container_title |
FEMS Microbiology Ecology |
container_volume |
49 |
container_issue |
3 |
container_start_page |
419 |
op_container_end_page |
432 |
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1766266090270228480 |